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Gabrb3 (NM_008071) Mouse Untagged Clone
CAT＃: MC216455
Gabrb3 (untagged) - Mouse gamma-aminobutyric acid (GABA) A receptor, subunit beta 3 (Gabrb3), transcript variant 1, (10ug)
CNY 5,488.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | A230092K12Rik; AW049585; beta3; Cp1; Gabrb-3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC216455 representing NM_008071
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGGGCTTTGCGGGAGGAAGGCTTTTCGGCATCTTCTCGGCCCCGGTGCTGGTGGCGGTGGTTTGCT GCGCTCAGAGCGTAAACGACCCCGGGAACATGTCCTTTGTGAAGGAGACGGTCGACAAGCTGTTGAAAGG CTACGACATTCGCCTGAGACCCGACTTCGGGGGTCCCCCAGTCTGCGTGGGGATGAACATCGACATCGCC AGCATCGACATGGTTTCTGAAGTCAACATGGATTATACCTTAACTATGTATTTCCAACAATACTGGAGAG ATAAAAGGCTCGCCTATTCTGGGATCCCTCTCAACCTCACGCTTGACAATCGAGTGGCTGACCAGCTCTG GGTGCCCGACACATATTTCTTAAATGACAAAAAGTCATTTGTCCACGGAGTGACAGTGAAAAACCGCATG ATCCGCCTCCACCCTGATGGGACAGTGCTGTATGGGCTCAGGATCACTACGACAGCAGCGTGCATGATGG ACCTCAGAAGATACCCACTGGATGAGCAAAACTGCACTTTGGAAATTGAAAGCTATGGCTACACTACGGA TGACATTGAATTTTACTGGCGTGGCGGGGACAAGGCTGTCACTGGCGTGGAAAGGATCGAGCTCCCACAG TTCTCCATTGTAGAGCACCGTCTGGTCTCCAGGAATGTTGTCTTCGCCACAGGTGCCTATCCTCGACTTT CATTGAGTTTTCGGTTGAAGAGAAATATCGGGTACTTCATTCTTCAGACGTATATGCCCTCAATCCTGAT CACAATCCTCTCGTGGGTGTCCTTCTGGATCAATTACGATGCATCTGCTGCTCGAGTTGCCCTTGGGATT ACCACCGTGCTCACCATGACAACCATCAACACTCACCTTCGGGAGACTCTACCCAAAATTCCCTATGTCA AAGCCATCGACATGTACCTGATGGGCTGCTTTGTCTTTGTATTCCTGGCACTTCTGGAGTACGCCTTTGT CAACTACATTTTCTTTGGAAGAGGTCCCCAAAGGCAGAAGAAGCTTGCGGAGAAGACAGCCAAGGCCAAG AATGATCGTTCTAAGAGTGAAATAAACCGGGTGGATGCTCACGGGAATATCCTATTAGCACCGATGGATG TTCACAATGAAATGAATGAGGTTGCAGGCAGCGTTGGTGACACCAGGAATTCAGCAATATCCTTTGACAA CTCAGGAATCCAGTATAGGAAACAGAGCATGCCCAAGGAAGGGCATGGGCGGTACATGGGAGACAGAAGC ATCCCGCACAAGAAGACCCACCTACGGAGGAGGTCTTCACAGCTCAAAATCAAAATCCCTGATCTAACCG ATGTGAATGCCATAGACAGATGGTCCCGCATCGTGTTTCCATTCACCTTTTCTCTCTTCAATTTAGTTTA CTGGCTGTACTATGTTAACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_008071 |
| Insert Size | 1422 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_008071.3, NP_032097.1 |
| RefSeq Size | 5568 bp |
| RefSeq ORF | 1422 bp |
| Locus ID | 14402 |
| UniProt ID | P63080 |
| Gene Summary | Ligand-gated chloride channel which is a component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the brain (PubMed:9108119). Plays an important role in the formation of functional inhibitory GABAergic synapses in addition to mediating synaptic inhibition as a GABA- gated ion channel (PubMed:27129275). The gamma2 subunit is necessary but not sufficient for a rapid formation of active synaptic contacts and the synaptogenic effect of this subunit is influenced by the type of alpha and beta subunits present in the receptor pentamer (PubMed:27129275). The alpha1/beta3/gamma2 receptor exhibits synatogenic activity whereas the alpha2/beta3/gamma2 receptor shows very little or no synaptogenic activity (PubMed:27129275). Functions also as histamine receptor and mediates cellular responses to histamine (By similarity). Plays an important role in somatosensation and in the production of antinociception (PubMed:10670447).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG222856 | Gabrb3 (tGFP-tagged) - Mouse gamma-aminobutyric acid (GABA) A receptor subunit beta 3 (Gabrb3) transcript variant 1, (10ug) |
CNY 3,710.00 |
|
| MR222856 | Gabrb3 (Myc-DDK-tagged) - Mouse gamma-aminobutyric acid (GABA) A receptor, subunit beta 3 (Gabrb3), transcript variant 1 |
CNY 5,488.00 |
|
| MR222856L3 | Lenti ORF clone of Gabrb3 (Myc-DDK-tagged) - Mouse gamma-aminobutyric acid (GABA) A receptor, subunit beta 3 (Gabrb3), transcript variant 1 |
CNY 5,230.00 |
|
| MR222856L4 | Lenti ORF clone of Gabrb3 (mGFP-tagged) - Mouse gamma-aminobutyric acid (GABA) A receptor, subunit beta 3 (Gabrb3), transcript variant 1 |
CNY 5,230.00 |
