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Sprtn (NM_001111141) Mouse Untagged Clone
CAT＃: MC217072
Sprtn (untagged) - Mouse predicted gene 505 (Gm505), (10ug)
CNY 6,460.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | Gm505 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC217072 representing NM_001111141
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGAGGATCTGGTGGTCGCGTTGCGGCTGCAGGAGGAGTGGGACGTGCAGATGGCGCGGCGCGCGG CGGCGGCGCGGGAGCCCGTGTCGTTGGTGGACGCGTCCTGGGAGCTGGTGGACCCCACCCCAGACCTGCA GGCGCTCTTCCTGCAGTTTAACGATCGCTTCTTCTGGGGTCAGCTGGAGGCGGTGGAGGTGAAGTGGAGT GTGCGCATGACCCTGTGTGCTGGGATCTGCACCTATGAAGGGAGAGGAGGCATGTGCTCCATCCGTCTCA GTGAGCCCCTGTTAAAACTGAGACCAAGAAAGGACCTTGTAGAGACTCTTTTGCATGAAATGATACATGC CTACTTATTTGTCACTAATAATGATAAAGACCGGGAAGGACACGGCCCAGAGTTCTGTAAACATATGCAT CGAATCAACCAACTGACAGGAGCCAACATAACGGTCTACCACACTTTCCATGATGAGGTGGATGAGTATC GCCGGCACTGGTGGCGCTGCAATGGACCCTGCCAGCACAGACAGCCCTACTACGGCTATGTGAAGCGTGC CACCAACAGGGCCCCCTCTGTTCATGACTACTGGTGGGCTGACCACCAGAAAACTTGTGGAGGCACTTAC ATAAAAATCAAGGAGCCAGAGAACTATTCGAAGAAAGGCAGAGGGAAGACAAAAGCAGACAAGCAGCCAG CATCGGCAGTGGAGAATAAAGACAAGCTGTGCAGAGGGGAGGCCCAGCTGCTCATCCCATTCAGTGGAAA AGGCTATGTTCTTGGAGACGCAAGCACTTGCCCTTCAGCTGGGAAGCTGAACACCTCATACATGGTTAAT GAAGCCAAAGGTCTCTCAAGTCAAGACCATTCAGTGAGTGGCCTGAGGCTCAATTCCAATGCTGAGGTGA AATGTGAACAGAACTGTCTCCCAAAGAAACCTCATCTTGTCAGCCCTCTTCCCACTGCTAGTCACCAAAG TGTCCTGAGCTCCTACTTCCCCAGAGTCTCTGTTGCCAACCAAAAGGCTTTCCGAAATGTGAATGGATCC CCAGTAAAGAACGGGACCACTGGGGATGGCACCAAGCGTCCTGCCTCAGGAGGCTCTCAGAGGAAGGTCC CACCTTCCAGGGCATCCCTGAGAAATACCTCCAAAGTCACAGCACCAGCGTCAGCAACCGTGACATCTGC AGCTGGGACATCCGCAACCATATCCCGAGAGGAGAGTGGGTCTGAAGACCAATTCCTGAACAAGCGACCC AGGTTGGAGGACAGGACTGCTTTGGATACTATCAAGGAACAAACACAAAGTGGTGGTGACCTTCGGAGTA GCTCACAGCCCACAGCCGCCAGCGCTCCTCAGAGCTTGAGCAGTCAACGGCGGCTGGTCAACTGTCCAGT GTGTCAGGGCGTGGTGGTGGAGTCACAGATTAACGAGCACTTGGACCGATGCCTGGAAGGTAACAAAACC AACCTGCGACCTCGAAGAGTGTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-RsrII |
| ACCN | NM_001111141 |
| Insert Size | 1494 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001111141.1, NP_001104611.1 |
| RefSeq Size | 2116 bp |
| RefSeq ORF | 1494 bp |
| Locus ID | 244666 |
| UniProt ID | G3X912 |
| Gene Summary | Regulator of UV-induced DNA damage response: acts as a 'reader' of ubiquitinated PCNA that enhances RAD18-mediated PCNA ubiquitination and translesion DNA synthesis (TLS). Recruited to sites of UV damage and interacts with ubiquitinated PCNA and RAD18, the E3 ubiquitin ligase that monoubiquitinates PCNA. Facilitates chromatin association of RAD18 and is required for efficient PCNA monoubiquitination, promoting a feed-forward loop to enhance PCNA ubiquitination and translesion DNA synthesis. Acts as a regulator of TLS by recruiting VCP/p97 to sites of DNA damage, possibly leading to extraction of DNA polymerase eta (POLH) by VCP/p97 to prevent excessive translesion DNA synthesis and limit the incidence of mutations induced by DNA damage (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG213725 | Sprtn (tGFP-tagged) - Mouse predicted gene 505 (Gm505), (10ug) |
CNY 3,710.00 |
|
| MR213725 | Sprtn (Myc-DDK-tagged) - Mouse predicted gene 505 (Gm505) |
CNY 3,656.00 |
|
| MR213725L3 | Lenti ORF clone of Sprtn (Myc-DDK-tagged) - Mouse predicted gene 505 (Gm505) |
CNY 5,230.00 |
|
| MR213725L4 | Lenti ORF clone of Sprtn (mGFP-tagged) - Mouse predicted gene 505 (Gm505) |
CNY 5,230.00 |
