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Wt1 (NM_144783) Mouse Untagged Clone
CAT＃: MC217294
Wt1 (untagged) - Mouse Wilms tumor 1 homolog (Wt1), (10ug)
CNY 3,880.00
CNY 6,650.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | D630046I19Rik; Wt-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC217294 representing NM_144783
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC GCGATCGCCCTGGACTTCCTCCTGTCGCAGGAGCCGGCTTCCACGTGTGTCCCGGAGCCGGCGTCTCAGC ACACGCTCCGCCGGGAGCCCGGGTGCGTCCAGCAGCCGGAGCAACCTGGGGACCGAGGCCCCCGGAGCGC CTGGGCCAAGTCCAGCGCCGAGAATCCGCAGGATCGCAGGAGCGGAGAACCGTCCGCATCCGAGCCGCAC CTCATGGGTTCCGACGTGCGGGACCTGAACGCGCTGCTGCCCGCTGTGTCTTCGCTGGGCGGCGGCGGCG GCGGCTGCGGGCTCCCTGTGAGCGGCGCAGCGCAGTGGGCGCCCGTGTTGGACTTCGCGCCTCCGGGCGC CTCGGCTTACGGGTCGCTGGGCGGTCCCGCGCCTCCTCCCGCTCCGCCGCCGCCTCCGCCGCCACCCCAC TCCTTCATCAAACAGGAGCCCAGCTGGGGCGGCGCCGAGCCACACGAGGAGCAGTGCCTGAGCGCCTTCA CCTTGCACTTCTCGGGCCAGTTCACCGGTACAGCCGGGGCCTGTCGCTACGGACCCTTCGGTCCTCCCCC GCCCAGCCAGGCGTCCTCGGGCCAGGCCAGGATGTTCCCCAATGCGCCCTACCTGCCCAGCTGCCTGGAG AGCCAGCCTACCATCCGCAACCAAGGATACAGCACGGTCACTTTCGACGGGGCGCCCAGCTATGGCCACA CGCCCTCGCATCACGCGGCGCAGTTCCCCAACCATTCCTTCAAACACGAGGACCCCATGGGCCAGCAGGG CTCGCTGGGCGAGCAGCAGTACTCCGTGCCACCTCCGGTGTATGGCTGCCACACCCCTACTGACAGTTGC ACAGGCAGCCAGGCCCTGCTCCTGAGGACGCCCTACAGCAGTGACAATTTATACCAAATGACCTCCCAGC TTGAATGCATGACCTGGAATCAGATGAACCTAGGAGCTACCTTAAAGGGAATGGCTGCTGGGAGCTCCAG CTCAGTGAAATGGACAGAAGGGCAGAGCAACCACGGCACAGGGTATGAGAGTGAGAACCACACGGCCCCC ATCCTCTGTGGTGCCCAGTACAGAATACACACCCACGGGGTCTTCCGAGGCATTCAGGATGTGCGGCGTG TATCTGGAGTGGCCCCAACTCTTGTCCGGTCAGCATCTGAAACCAGTGAGAAACGTCCTTTCATGTGTGC ATACCCAGGCTGCAATAAGAGATATTTTAAGCTGTCCCACTTACAGATGCATAGCCGGAAGCACACTGGT GAGAAACCATACCAGTGTGACTTCAAGGACTGCGAGAGAAGGTTTTCTCGCTCAGACCAGCTCAAAAGAC ACCAAAGGAGACACACAGGTGTGAAACCATTCCAGTGTAAAACTTGTCAGCGAAAGTTTTCCCGGTCCGA CCATCTGAAGACCCACACCAGGACTCATACAGGTAAAACAAGTGAAAAGCCCTTCAGCTGTCGGTGGCAC AGTTGTCAGAAAAAGTTTGCGCGCTCAGACGAATTGGTCCGCCATCACAACATGCATCAGAGAAACATGA CCAAACTCCAGCTGGCGCTTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_144783 |
| Insert Size | 1563 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_144783.2, NP_659032.3 |
| RefSeq Size | 3092 bp |
| RefSeq ORF | 1554 bp |
| Locus ID | 22431 |
| Gene Summary | This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It plays an essential role in the normal development of the urogenital system, and the orthologous human gene is mutated in a small subset of patients with Wilm's tumors. Alternative splicing has been noted for this gene, however, the full-length nature of these variants is not known. The mRNA for this gene has been shown to initiate translation from non-AUG (CUG) and AUG translation start sites, resulting in different isoforms. [provided by RefSeq, Apr 2013] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG225851 | Wt1 (tGFP-tagged) - Mouse Wilms tumor 1 homolog (Wt1), (10ug) |
CNY 7,728.00 |
|
| MR225851 | Wt1 (Myc-DDK-tagged) - Mouse Wilms tumor 1 homolog (Wt1) |
CNY 6,128.00 |
|
| MR225851L3 | Lenti ORF clone of Wt1 (Myc-DDK-tagged) - Mouse Wilms tumor 1 homolog (Wt1) |
CNY 7,410.00 |
|
| MR225851L4 | Lenti ORF clone of Wt1 (mGFP-tagged) - Mouse Wilms tumor 1 homolog (Wt1) |
CNY 8,528.00 |
