Lrp4 (NM_001145857) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	6430526J12Rik; D230026E03; mdig; Megf7
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC217359 representing NM_001145857 Red=Cloning site Blue=ORF TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGCGGTGGTGGGGCGCGCTGCTGCTTGGCGCCCTGCTCTGCGCACACGGAATAGCCAGCAGTCTGG AGTGTGCATGTGGTCGGAGCCACTTCACATGTGCGGTGAGCGCGCTTGGCGAATGTACCTGTATCCCCGC CCAGTGGCAGTGTGATGGAGACAATGACTGTGGAGACCACAGCGATGAGGATGGCTGTACGCTGCCTACC TGCTCCCCTCTGGACTTCCACTGTGACAACGGCAAGTGCATCCGACGCTCCTGGGTGTGTGACGGGGACA ATGACTGTGAAGATGACTCTGATGAGCAGGACTGTCCCCCCCGGGAGTGTGAGGAGGACGAATTCCCCTG CCAGAACGGCTACTGCATTCGGAGTCTGTGGCACTGTGATGGGGACAACGACTGTGGTGACAACAGTGAC GAGCAATGCGACATGCGCAAGTGCTCAGACAAGGAATTCCGCTGCAGCGACGGCAGCTGCATTGCTGAGC ATTGGTACTGCGATGGTGACACAGACTGCAAAGATGGCTCTGATGAGGAAAGCTGCCCTTCAGCAGTACC CTCTCCTCCTTGCAACCTGGAAGAGTTCCAGTGCGCCTATGGCCGCTGCATCCTCGACATCTACCACTGT GACGGAGATGACGACTGTGGAGACTGGTCAGACGAGTCTGACTGCTCCTCCCACCAGCCCTGCCGCTCTG GGGAATTCATGTGTGACAGTGGCCTGTGCATCAACTCGGGCTGGCGCTGTGATGGTGATGCAGACTGTGA TGACCAGTCTGATGAACGCAACTGCACCACCTCCATGTGCACAGCCGAACAGTTTCGCTGTCGGTCAGGC CGTTGCGTCCGTCTGTCCTGGCGCTGTGATGGGGAGGATGACTGTGCGGACAACAGCGATGAAGAAAACT GTGAGAACACAGGAAGCCCCCAGTGCGCCTCTGACCAGTTCCTGTGTTGGAACGGGCGCTGTATTGGGCA GAGGAAGCTGTGCAACGGGATCAACGACTGCGGTGACAGCAGTGACGAAAGTCCACAGCAGAACTGTCGG CCCCGGACGGGTGAGGAGAACTGCAATGTTAACAACGGTGGCTGTGCCCAGAAGTGTCAGATGGTGCGAG GGGCAGTGCAGTGTACCTGCCACACAGGCTACCGGCTCACAGAGGATGGGAGAACGTGCCAGGATGTAAA CGAATGTGCTGAGGAGGGGTACTGCAGCCAGGGCTGCACAAACACTGAAGGGGCTTTCCAGTGCTGGTGT GAAGCAGGGTATGAGCTACGGCCCGATCGGCGCAGCTGCAAAGCTCTGGGGCCAGAACCTGTGCTACTGT TTGCCAATCGTATCGACATCCGGCAGGTGCTCCCGCACCGCTCCGAGTACACGCTGCTACTGAACAACCT GGAGAACGCCATTGCCCTCGACTTTCATCACCGGCGGGAGCTGGTCTTCTGGTCGGATGTCACCCTGGAC CGCATCCTCCGTGCCAACCTTAATGGCAGCAATGTAGAGGAGGTGGTGTCTACTGGGCTAGAGAGCCCAG GTAGGAAAACAAGGCCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI
ACCN	NM_001145857
Insert Size	1560 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001145857.1, NP_001139329.1
RefSeq Size	3246 bp
RefSeq ORF	1560 bp
Locus ID	228357
UniProt ID	Q8VI56
Gene Summary	Mediates SOST-dependent inhibition of bone formation. Functions as a specific facilitator of SOST-mediated inhibition of Wnt signaling. Plays a key role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between motor neuron and skeletal muscle. Directly binds AGRIN and recruits it to the MUSK signaling complex. Mediates the AGRIN-induced phosphorylation of MUSK, the kinase of the complex. The activation of MUSK in myotubes induces the formation of NMJ by regulating different processes including the transcription of specific genes and the clustering of AChR in the postsynaptic membrane. Alternatively, may be involved in the negative regulation of the canonical Wnt signaling pathway, being able to antagonize the LRP6-mediated activation of this pathway. More generally, has been proposed to function as a cell surface endocytic receptor binding and internalizing extracellular ligands for degradation by lysosomes. Plays an essential role in the process of digit differentiation (PubMed:16517118).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1.

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