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Grhl1 (NM_145890) Mouse Untagged Clone
CAT＃: MC217636
Grhl1 (untagged) - Mouse grainyhead-like 1 (Drosophila) (Grhl1), transcript variant 2, (10ug)
CNY 6,840.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | LBP-32; MGR; Tcfcp2l2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC217636 representing NM_145890
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTCTCCTTGATGATGAACTGTGTGACCTAAACAGCATACCAAATGTGACGGAGCAGCCCCTCATTT CTGCTGGAGAAAACAGGGTGCAAGTGCTGAAAAACGTGCCCTTCAACATCGTCCTCCCCCATAGCAACCA GCTGGGCATTGATAAGAGAGGCCATCTGACAGCTCCCGATACAACAGTCACTGTCTCCATAGCAACCATG CCTACCCACTCCATCAAGACAGAAATCCAGCCGCACGGCTTTGCTGTGGGAATCCCACCAGCCGTGTACC ACTCTGAGCCCACCGAACGTGTGGTGGTTTTTGACCGGAGCCTCAGCACTGATCAGTTCAGCTCTGGCAC TCAGCCCCCCAATGCTCAGCGGAGGACTCCAGACTCCACCTTCTCCGAGACCTTCAAGGAGGGCGTTCAG GAGGTTTTCTTCCCCTCGGAACTCAGCCTTCGGATGCCGGGCATGAATTCAGAGGACTATGTCTTTGACA ATGTTTCTGGGAACAACTTTGAGTATACCCTGGAAGCCTCCAAGTCACTGCGGCAGAAGCAAGGGGACAG CACTATGACATACCTGAATAAAGGCCAGTTCTATCCTGTCACCTTAAAGGAAGGAAGCAGCAATGAAGGG ATTCACCACCCTATCAGCAAAGTTCGAAGTGTGATCATGGTGGTTTTTGCTGAAGACAAAAGCAGAGAAG ACCAGCTGAGACACTGGAAGTACTGGCACTCCCGTCAGCACACGGCCAAACAGAGGTGCATTGACATTGC TGACTACAAAGAAAGCTTCAACACTATCAGCAACATTGAGGAGATAGCTTATAACGCCATTTCCTTCACG TGGGACATCAATGATGAGGCAAAGGTCTTCATCTCTGTGAACTGCTTGAGCACAGATTTCTCTTCTCAGA AGGGTGTGAAGGGCTTGCCACTCAACATTCAAATCGACACATACAGCTATAACAACCGCAGCAACAAGCC GGTTCACCGGGCCTACTGCCAGATAAAGGTCTTCTGCGACAAGGGAGCTGAAAGGAAAATTCGGGATGAA GAACGAAAACAGAGCAAGAGAAAAGTGTCTGACGTTAAAGTGCAGCTGCTTCCCTCGCACAAACGGACAG ACATCACAGTGTTCAAGCCCTTCCTGGACCTCGACACTCAGCCTGTCCTCTTCATTCCGGACGTGCATTT TACCAACCTGCAGCGGGGCAGTCATGTTCTTTCCCTCCCCTCTGAAGAACTGGAAGGTGAAGGCTCTGTC TTGAAAAGAGGGCCATTCGGAACCGAAGATGACTTTGGAGTTCCTCCTCCTGCTAAGCTGACTCGGACAG AAGAACCCAAGAGAGTGCTGCTCTATGTCCGAAAGGAATCAGAAGAAGTCTTCGACGCCCTGATGCTCAA GACGCCGTCTTTGAAGGGCCTGATGGAGGCAATTTCAGACAAGTATGATGTCCCCCATGACAAGATTGGG AAAATATTTAAGAAGTGCAAAAAAGGGATCCTCGTGAACATGGACGACAACATTGTGAAGCACTACTCCA ATGAGGACACCTTCCAGCTGCAGATAGAGGAAGCCGGCGGCTCGTACAAGCTCACCCTGACAGAGATTTA A ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_145890 |
| Insert Size | 1611 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_145890.2, NP_665897.2 |
| RefSeq Size | 3100 bp |
| RefSeq ORF | 1611 bp |
| Locus ID | 195733 |
| Gene Summary | Transcription factor involved in epithelial development. Binds directly to the consensus DNA sequence 5'-AACCGGTT-3' (PubMed:18288204, PubMed:21081122). Important regulator of DSG1 in the context of hair anchorage and epidermal differentiation, participates in the maintenance of the skin barrier (PubMed:18288204, PubMed:24586629). There is no genetic interaction with GRHL3 no genetic interaction with GRHL3, no functional cooperativity due to diverse target gene selectivity during epithelia development (PubMed:21081122).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR and coding region, and initiates translation at an alternate start codon compared to variant 1. The encoded isoform 92) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG223939 | Grhl1 (tGFP-tagged) - Mouse grainyhead-like 1 (Drosophila) (Grhl1) transcript variant 2, (10ug) |
CNY 5,890.00 |
|
| MR223939 | Grhl1 (Myc-DDK-tagged) - Mouse grainyhead-like 1 (Drosophila) (Grhl1), transcript variant 2 |
CNY 5,320.00 |
|
| MR223939L3 | Lenti ORF clone of Grhl1 (Myc-DDK-tagged) - Mouse grainyhead-like 1 (Drosophila) (Grhl1), transcript variant 2 |
CNY 7,220.00 |
|
| MR223939L4 | Lenti ORF clone of Grhl1 (mGFP-tagged) - Mouse grainyhead-like 1 (Drosophila) (Grhl1), transcript variant 2 |
CNY 7,220.00 |
