Polr1e (BC034783) Mouse Untagged Clone
CAT#: MC217814
Polr1e (untagged) - Mouse polymerase (RNA) I polypeptide E (cDNA clone MGC:35831 IMAGE:4022372), (10ug)
CNY 6,940.00
Product images
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | 53kDa; AU042259; D030019D19Rik; Paf53; Praf1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>BC034783
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCCCTGGAGTCTCCTGGGATGGATGACCAGGCTGGAGACACGGAGACAGAGGCGCTACAGAGTG CAAGGTGGCTGTACTGTGGAGAGCCCGACGACAGGCAGAAAGCTGTGCTGGTCCAGTTCTCCAATGGGAA GCTGCAGAATCCGGGCGACATGCGCTTCACCTTGTACAACAGCACCGACTTAGTAAACCCCAGGCAGAGG AGTCACCGGATCGTGGCAGCTGAAACAGACAGGCTCTCCTATGTCGGAAACAATTTTGGGACCGGTGCCC TCAAATGCAATGCTCTTTGCAGACACTTTGTGGGAATTCTGAATAAGACCTCTGGCCAGATGGAAGTCTA TGATGCCGAACTGTTCAACATGCAGCCGCTGTTTGCTGGAATGGGAACTGAAGTCATCAAACTTGGTGGC CAACACCTTTACCTGCTGGCCTTCTGCCAACCCTCCAAGAACCTGGCTGAGGCAGGTGACCTGCTGCTGA GCCGACACCGGCAAGGCCATTGCATTGCAGTCCTGCTGGATGACGATGCTATTGAGCGAGAGCCTCCTCT GGAGAATCAGAACAAGACTTTCAGGGACAAGTTGGATTCTTGCATTGAAGCCTTTGGAAGCACCAAGCAG AAGCGGTCCCTAAACTCCAGGCGGATGAACAAAGTTGGCAGCGAGTCTCTGAATCTCAGTGTGGCGAAAG CCGCGGAGAGTATCATTGATACAAAGGGTGTGAATGCGCTGGTCAGTGATGCCATGCAGGATGACTTACA ATATGACGCCCTCTACCTTCCTCCCTGCTACGCTGACGCAGCCAAGCCGGAAGACGTGTATAGATTTGAA GACATTCTGTCCCCGGCCGAGTATGATGCTCTTGAGAGCCCATCCGAAGCCTTCAGAAAGGTCACATCGG AAGACATACTGAAGATGATTGAAGAGAACAGCCACTGCTCCTACGTCATAGAAATGCTGAAGTCTCTGCC CATAGATGAGGTCCACCGGAACCGGCAAGCCCGGAGCATCTGGTTCCTAGACGCCCTCATCAGATTCCGA GCTCAGAAAGTGATAAAGGGGAAAAGAGCCTTAGGGCCTGGAATCCCGCACATTATCAACACCAAGCTGC TGAAACAGTTCACCTGCTTGACCTACAACAACGGCAGGTTACAGAACCTAATTTCAAGTTCTATGAGGGC CAAGATCACTTCGTATGCGATCATACTTGCCCTGCACATAAATAACTTCCAGGTTGACCTGACAGCGTTG CAGAAGGACTTGAAGCTGAGTGAGAAAAGGATTATTGAGATAGCGAAAGCCATGAGGCTGAAGATCTCCA AACAAAAGGTGTCCCTGGCAGATGGCAGGGAAGAGAGTCACAGACTGGGCACTCTGTCTGTCCCACTGCC TCCAGCCCAGAACTCTGATCGACAGTCAAAGCGGAGGAAAATGAACTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | BC034783 |
Insert Size | 1449 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | BC034783, AAH34783 |
RefSeq Size | 1757 bp |
RefSeq ORF | 1448 bp |
Locus ID | 64424 |
Gene Summary | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG207725 | Polr1e (tGFP-tagged) - Mouse polymerase (RNA) I polypeptide E (cDNA clone MGC:35831 IMAGE:4022372) |
CNY 3,040.00 |
|
MR207725 | Polr1e (Myc-DDK-tagged) - Mouse polymerase (RNA) I polypeptide E (cDNA clone MGC:35831 IMAGE:4022372) |
CNY 3,656.00 |
|
MR207725L3 | Lenti ORF clone of Polr1e (Myc-DDK-tagged) - Mouse polymerase (RNA) I polypeptide E (cDNA clone MGC:35831 IMAGE:4022372) |
CNY 4,660.00 |
|
MR207725L4 | Lenti ORF clone of Polr1e (mGFP-tagged) - Mouse polymerase (RNA) I polypeptide E (cDNA clone MGC:35831 IMAGE:4022372) |
CNY 4,660.00 |