Polr2c (BC002023) Mouse Untagged Clone

CAT#: MC218102

Polr2c (untagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide C (cDNA clone MGC:5895 IMAGE:3492868), (10ug)



  "BC002023" in other vectors (4)

CNY 6,940.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms mRBP31, 33kDa, RPB3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC002023
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGTACGCCAACCAGCCAACAGTGCGGATCACGGAACTCACCGAAGAGAACGTCAAGTTCATTATCG
AGAACACGGACCTGGCGGTGGCCAATTCCATTCGGAGGGTCTTCATCGCAGAGGTGCCCATAATAGCCAT
CGACTGGGTTCAGATTGACGCCAACTCCTCGGTCCTTCATGATGAATTCATCGCGCACAGGCTTGGTTTA
ATCCCCCTCACTAGTGATGACATTGTGGACAAGCTGCAGTACTCCCGGGACTGCACATGTGAAGAGTTCT
GCCCTGAGTGTTCTGTGGAGTTCACTCTCGATGTGAGGTGCAATGAAGACCAGACGCGCCATGTCACCTC
CCGAGATCTCATCTCCAACAGCCCCCGGGTTATTCCGGTGACATCTCGGAACCGGGATAACGACCCTAAT
GACTATGTGGAGCAGGATGACATCCTTATTGTCAAGCTGAGGAAGGGGCAGGAGCTGAGACTTCGAGCCT
ATGCCAAGAAGGGCTTTGGCAAGGAACATGCCAAGTGGAACCCTACTGCGGGGGTAGCTTTTGAATACGA
TCCAGACAACGCACTTCGGCATACAGTGTATCCCAAGCCTGAGGAATGGCCCAAGAGTGAGTATTCCGAG
CTGGATGAGGATGAGTCGCAGGCTCCCTACGACCCCAACGGCAAGCCAGAGAGGTTTTACTACAATGTGG
AGTCGTGTGGCTCACTGCGCCCTGAAACCATTGTCCTCTCAGCCCTGTCGGGGCTAAAGAAGAAGCTGAG
TGATTTACAGACTCAGTTAAGCCATGAGATCCAGAGTGACGTGCTCACCATAAACTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Restriction Sites SgfI-MluI     
ACCN BC002023
Insert Size 828 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC002023, AAH02023
RefSeq Size 1207 bp
RefSeq ORF 827 bp
Locus ID 20021
Gene Summary DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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