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Ubb (BC100341) Mouse Untagged Clone
CAT＃: MC218265
Ubb (untagged) - Mouse ubiquitin B (cDNA clone MGC:117670 IMAGE:30787432), (10ug)
CNY 6,940.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AL033289; Rps27a; Uba52; Ubb2; Ubc |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC100341
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGATCTTCGTGAAGACCCTGACCGGCAAGACCATCACCCTAGAGGTGGAGCCCAGTGACACCATCG AGAACGTGAAGGCCAAGATCCAGGATAAAGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCCGG CAAGCAGCTGGAAGATGGCCGCACCCTCTCTGATTACAACATCCAGAAGGAGTCAACCCTGCACCTGGTC CTTCGCCTGAGAGGTGGCATGCAGATCTTCGTGAAGACCCTGACCGGCAAGACCATCACCCTGGAGGTGG AGCCCAGTGACACCATCGAGAACGTGAAGGCCAAGATCCAGGATAAAGAGGGCATCCCCCCTGACCAGCA GAGGCTGATCTTTGCCGGCAAGCAGCTGGAAGATGGCCGCACCCTCTCTGATTACAACATCCAGAAAGAG TCAACCCTGCACCTGGTCCTCCGTCTGAGGGGTGGCATGCAGATCTTCGTGAAGACCCTGACTGGCAAGA CCATCACCCTGGAGGTGGAGCCCAGTGACACCATCGAGAACGTGAAGGCCAAGATCCAGGATAAAGAGGG CATCCCCCCTGACCAGCAGAGGCTGATCTTTGCCGGCAAGCAGCTGGAAGATGGCCGCACCCTCTCTGAT TACAACATCCAGAAGGAGTCAACCCTGCACCTGGTCCTTCGCCTGAGAGGTGGCATGCAGATCTTCGTGA AGACCCTGACCGGCAAGACCATCACCTTGGAGGTGGAGCCCAGTGACACCATCGAGAATGTGAAGGCCAA GATCCAGGATAAAGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCCGGCAAGCAGCTAGAAGAT GGCCGCACTCTCTCTGATTACAACATCCAGAAAGAGTCGACCCTGCACCTGGTCCTCCGTCTGAGGGGTG GCTATTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | BC100341 |
| Insert Size | 918 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC100341, AAI00342 |
| RefSeq Size | 1145 bp |
| RefSeq ORF | 917 bp |
| Locus ID | 22187 |
| Gene Summary | This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene consists of four direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Pseudogenes of this gene are located on chromosomes 3 and 14. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG204272 | Ubb (tGFP-tagged) - Mouse ubiquitin B (cDNA clone MGC:117670 IMAGE:30787432) |
CNY 2,850.00 |
|
| MR204272 | Ubb (Myc-DDK-tagged) - Mouse ubiquitin B (cDNA clone MGC:117670 IMAGE:30787432) |
CNY 2,400.00 |
|
| MR204272L3 | Lenti ORF clone of Ubb (Myc-DDK-tagged) - Mouse ubiquitin B (cDNA clone MGC:117670 IMAGE:30787432) |
CNY 4,750.00 |
|
| MR204272L4 | Lenti ORF clone of Ubb (mGFP-tagged) - Mouse ubiquitin B (cDNA clone MGC:117670 IMAGE:30787432) |
CNY 4,750.00 |
