Has2 (NM_008216) Mouse Untagged Clone

CAT#: MC218773

Has2 (untagged) - Mouse hyaluronan synthase 2 (Has2), (10ug)



  "NM_008216" in other vectors (6)

CNY 7,030.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC218773 representing NM_008216
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCATTGTGAGAGGTTTCTATGTGTCCTGAGAATAATTGGAACTACACTTTTTGGAGTGTCTCTCCTCC
TCGGAATCACAGCTGCTTATATTGTTGGCTACCAGTTTATCCAAACAGATAATTACTACTTCTCATTTGG
ACTGTACGGTGCCTTTTTAGCCTCGCATCTCATCATCCAAAGCCTCTTTGCCTTTTTGGAACACCGGAAA
ATGAAGAAGTCCCTTGAAACCCCGATTAAATTGAACAAAACGGTAGCACTCTGCATCGCTGCGTACCAAG
AGGACCCTGACTACTTACGGAAATGTTTGCAATCTGTGAAAAGGCTGACCTACCCTGGGATTAAAGTCGT
GATGGTCATCGATGGGAACTCAGACGACGACCTTTACATGATGGACATATTCAGCGAAGTTATGGGCAGG
GACAAATCGGCCACGTACATCTGGAAGAACAACTTTCATGAAAAGGGACCTGGTGAGACAGAAGAGTCCC
ATAAAGAAAGTTCACAACATGTCACCCAATTGGTCTTGTCTAACAAAAGTATTTGCATCATGCAAAAATG
GGGTGGAAAGAGAGAAGTCATGTACACAGCCTTCAGAGCACTGGGGCGAAGCGTGGATTATGTACAGGTG
TGTGACTCAGATACTATGCTTGACCCTGCCTCATCTGTGGAGATGGTGAAGGTCTTAGAGGAAGACCCTA
TGGTTGGAGGTGTTGGAGGAGATGTCCAGATTTTAAACAAGTATGATTCCTGGATCTCCTTCCTCAGCAG
CGTGAGATACTGGATGGCTTTTAATATAGAAAGGGCCTGCCAGTCTTATTTTGGCTGTGTCCAGTGCATA
AGCGGTCCTCTGGGAATGTACAGAAACTCCTTGCTGCATGAATTTGTGGAAGACTGGTACAATCAGGAAT
TCATGGGTAACCAATGCAGTTTTGGTGACGACAGGCACCTTACCAACAGGGTGTTGAGTCTGGGCTATGC
AACTAAATACACGGCTCGGTCCAAGTGCCTTACTGAAACTCCCATAGAATATCTGAGATGGCTGAACCAG
CAGACCCGTTGGAGCAAGTCCTACTTCCGAGAGTGGCTGTACAATGCCATGTGGTTTCACAAGCATCACT
TGTGGATGACCTATGAAGCTGTTATCACTGGATTCTTTCCTTTCTTTCTCATTGCCACAGTCATCCAGCT
CTTCTACAGGGGTAAAATCTGGAACATCCTCCTCTTCCTGTTAACTGTCCAGCTAGTGGGTCTCATCAAG
TCATCTTTTGCCAGCTGCCTTAGAGGAAATATCGTCATGGTATTCATGTCTCTGTATTCAGTGTTATACA
TGTCAAGTCTACTTCCTGCCAAGATGTTTGCAATTGCAACCATAAACAAAGCTGGGTGGGGCACATCTGG
AAGGAAGACCATTGTTGTTAATTTCATAGGACTTATTCCAGTGTCCGTGTGGTTTACAATCCTTCTAGGT
GGTGTAATTTTCACCATTTATAAGGAATCTAAAAAGCCATTTTCCGAATCCAAACAGACTGTTCTCATCG
TGGGAACTTTGATCTATGCATGCTACTGGGTCATGCTTTTGACTCTCTATGTGGTTCTCATCAATAAGTG
TGGCAGGCGGAAGAAGGGACAACAGTATGACATGGTGCTTGATGTATGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_008216
Insert Size 1659 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_008216.3, NP_032242.3
RefSeq Size 4262 bp
RefSeq ORF 1659 bp
Locus ID 15117
UniProt ID P70312
Gene Summary Catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer. Therefore, it is essential to hyaluronan synthesis a major component of most extracellular matrices that has a structural role in tissues architectures and regulates cell adhesion, migration and differentiation. This is one of the isozymes catalyzing that reaction and it is particularly responsible for the synthesis of high molecular mass hyaluronan. Required for the transition of endocardial cushion cells into mesenchymal cells, a process crucial for heart development. May also play a role in vasculogenesis. High molecular mass hyaluronan also play a role in early contact inhibition a process which stops cell growth when cells come into contact with each other or the extracellular matrix.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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