Psap (NM_001146122) Mouse Untagged Clone
CAT#: MC218818
Psap (untagged) - Mouse prosaposin (Psap), transcript variant 4, (10ug)
CNY 7,030.00
Product images
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Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | AI037048; SGP; SGP-1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC218818 representing NM_001146122
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACGCCCTCGCCCTCTTCGCCAGCCTTCTGGCCACCGCTCTGACCAGCCCTGTCCAAGACCCGAAGA CATGCTCTGGGGGCTCAGCAGTGCTGTGCAGAGATGTGAAGACGGCGGTGGACTGTGGGGCCGTGAAGCA CTGCCAGCAGATGGTCTGGAGCAAGCCCACAGCGAAATCCCTTCCTTGCGACATATGCAAAACTGTTGTC ACCGAAGCTGGGAACTTGCTGAAAGATAATGCTACGCAGGAGGAGATCCTTCATTACCTGGAGAAGACCT GTGAGTGGATTCATGACTCCAGCCTGTCGGCCTCGTGCAAGGAGGTGGTTGACTCTTACCTGCCTGTCAT CCTGGACATGATTAAGGGCGAGATGAGCAACCCTGGGGAAGTGTGCTCTGCGCTCAACCTCTGCCAGTCC CTTCAGGAGTACTTGGCCGAGCAAAACCAGAAACAGCTTGAGTCCAACAAGATCCCGGAGGTGGACATGG CCCGTGTGGTTGCCCCCTTCATGTCCAACATCCCTCTCCTGCTGTACCCTCAGGATCACCCCCGCAGCCA GCCCCAACCTAAGGCTAACGAGGACGTCTGCCAGGACTGTATGAAGCTGGTGTCTGATGTCCAGACTGCT GTGAAGACCAACTCCAGCTTTATCCAGGGCTTCGTGGACCACGTGAAGGAGGATTGTGACCGCTTGGGGC CAGGCGTGTCTGACATATGCAAGAACTACGTGGACCAGTATTCCGAGGTCTGTGTCCAGATGTTGATGCA CATGCAACCCAAGGAAATCTGTGTGCTGGCTGGCTTCTGTAATGAGGTCAAGAGAGTGCCAATGAAGACT CTGGTCCCTGCCACCGAGACCATTAAGAACATCCTCCCTGCCCTGGAGATGATGGACCCCTATGAGAATC TGGTCCAGGCCCACAATGTGATTTTATGCCAGACCTGTCAGTTTGTGATGAATAAGTTTTCTGAGCTGAT TGTCAATAATGCCACTGAGGAGCTCCTAGTTAAAGGTTTGAGCAACGCATGCGCACTGCTCCCCGATCCT GCCAGAACCAAGTGCCAGGAGGTGGTGGGAACATTTGGCCCCTCCCTGTTGGACATCTTTATCCATGAGG TAAACCCCAGCTCTCTGTGCGGTGTGATCGGCCTCTGTGCTGCCCGCCCGGAGTTGGTGGAGGCACTTGA GCAGCCTGCGCCAGCCATTGTATCTGCACTGCTCAAAGAGCCCACACCGCCAAAGCAGCCCGCACAGCCC AAGCAGTCGGCATTGCCCGCCCATGTGCCTCCTCAGAAGAATGGTGGGTTCTGTGAGGTGTGCAAGAAAC TGGTCCTCTATTTGGAACATAACCTGGAGAAAAACAGCACCAAGGAGGAAATCCTGGCCGCACTTGAGAA GGGCTGCAGCTTCCTGCCAGACCCTTACCAGAAGCAGTGCGATGACTTTGTGGCTGAGTATGAGCCCTTG CTATTGGAGATCCTCGTGGAAGTGATGGATCCTGGATTTGTGTGCTCGAAAATTGGAGTTTGCCCTTCTG CCTATAAGCTGCTGCTGGGAACCGAGAAGTGTGTCTGGGGCCCTAGCTACTGGTGTCAGAACATGGAGAC TGCCGCCCGATGCAATGCTGTCGATCATTGCAAACGCCATGTGTGGAACTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001146122 |
Insert Size | 1662 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001146122.1, NP_001139594.1 |
RefSeq Size | 2664 bp |
RefSeq ORF | 1662 bp |
Locus ID | 19156 |
UniProt ID | Q61207 |
Gene Summary | This gene encodes a multifunctional glycoprotein that plays a role in the intracellular metabolism of various sphingolipids or secreted into the plasma, milk or cerebrospinal fluid. The encoded protein undergoes proteolytic processing to generate four different polypeptides known as saposin A, B, C or D, that are required for the hydrolysis of certain sphingolipids by lysosomal hydrolases. Alternately, the encoded protein is secreted into body fluids where it exhibits neurotrophic and myelinotrophic activities. A complete lack of the encoded protein is fatal to mice either at the neonatal stage or within the first month due to severe leukodystrophy and sphingolipid accumulation. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate the mature saposins. [provided by RefSeq, Sep 2015] Transcript Variant: This variant (4) lacks an exon in the coding region and uses an alternate in-frame splice site, compared to variant 2, resulting in a shorter protein (isoform D), compared to isoform B. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG227325 | Psap (tGFP-tagged) - Mouse prosaposin (Psap) transcript variant 4, (10ug) |
CNY 6,080.00 |
|
MR227325 | Psap (Myc-DDK-tagged) - Mouse prosaposin (Psap), transcript variant 4 |
CNY 5,510.00 |
|
MR227325L3 | Lenti ORF clone of Psap (Myc-DDK-tagged) - Mouse prosaposin (Psap), transcript variant 4 |
CNY 7,410.00 |
|
MR227325L4 | Lenti ORF clone of Psap (mGFP-tagged) - Mouse prosaposin (Psap), transcript variant 4 |
CNY 7,410.00 |