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Nars (NM_027350) Mouse Untagged Clone
CAT＃: MC218924
Nars (untagged) - Mouse asparaginyl-tRNA synthetase (Nars), transcript variant 2, (10ug)
CNY 7,130.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 3010001M15Rik; AA960128; ASNRS; C78150 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC218924 representing NM_027350
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCTCGGAGGTGATTAGGGGGACTGCAGAGATGGTTCTAGAGCTGTATGTATCTGACCGAGAAGGAA ATGACGCAACCGGGGATGGAACCAAGGAGAAACCATTTAAAACAGGCCTAAAGGCTTTGATGACAGTTGG AAAAGAGCCATTTCCCACCATTTATGTGGACTCACAAAAGGAAAATGAGAGATGGGATGTTATTTCTAAG TCACAGATGAAGAACATTAAAAAGATGTGGCACAGAGAACAGATGAAGAACGACTCTAGGGAGAAGAAAG AGGCAGAAGATAACTTACGAAGAGAAAAGAACCTGGAGGAAGCAAAAAAAATTATTATTAAAAATGACCC GAGCCTGCCAGAGCCGGCCTGTGTAAAGATTAGTGCATTAGAAGGATACAGAGGCCAGAGAGTGAAGGTG TTTGGCTGGGTCCACAGGTTGCGCAGGCAAGGAAAGAATTTGATGTTTTTGGTGTTGCGAGATGGTACGG GTTATCTTCAGTGTGTCTTGTCAGATGACTTGTGTCAGTGTTACAATGGAGTAGTCCTGTCCACCGAGAG TAGTGTGGCGGTGTACGGAACACTAAACCTAACTCCAAAGGGCAAACAGGCTCCAGGAGGCCATGAGCTG AGCTGTGACTTCTGGGAACTGGTGGGGCTGGCCCCGGCTGGAGGAGCTGATAACCTGATCAACGAGGAGT CTGATGTGGATGTCCAGCTCAACAACCGGCACATGATGATCCGGGGAGAGAACATGTCCAAAATCCTGAA AGCGCGCTCCATGATCACCAGGTGTTTCCGGGACCACTTCTTCGACAGGGGCTACTGTGAAGTTACCACT CCAACACTGGTGCAGACGCAGGTGGAAGGTGGGGCCACGCTCTTCAAGCTTGACTATTTCGGGGAAGAAG CGTTTTTGACCCAGTCCTCACAGTTGTACCTGGAGACCTGCCTTCCAGCCCTGGGAGATGTTTTTTGTAT AGCCCAGTCATACAGGGCCGAACAGTCCAGGACACGAAGGCACCTGGCTGAGTTCACTCACGTGGAAGCT GAGTGTCCTTTCCTGACCTTCGAGGACCTCCTGAACCGTTTAGAGGACCTAGTGTGTGATGTGGTGGACA GAGTCCTGAAGTCACCAGTGGCAAGCATAGTGTATGAGCTCAACCCGAACTTTAAACCCCCCAAGCGGCC TTTCCGCAGGATGAACTACTCGGATGCTATTGAGTGGCTGAAGGAGCATGATGTCAAGAAAGAAGACGGG ACTTTCTACGAGTTTGGAGATGATATTCCCGAAGCGCCTGAGAGACTGATGACAGACACCATTAATGAAC CAATCCTGCTGTGTCGCTTTCCTGTGGAGATCAAGTCTTTCTATATGCAGCGCTGTCCTGAGGATCCTCG ACTTACTGAATCTGTGGACGTGTTGATGCCCAACGTTGGTGAGATTGTAGGTGGCTCGATGCGCTCCTGG GACAGTGAGGAGATTCTCGAAGGCTATAAGAGGGAAGGGATTGACCCCGCTCCTTACTACTGGTATACAG ATCAGAGAAAATATGGCACCTGTCCTCATGGAGGGTATGGCTTGGGCTTGGAACGATTTCTTAGCTGGAT TCTGAACAGGTATCACATCCGAGATGTGTGCCTGTACCCTCGATTTCTCCAGCGCTGCAGGCCATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_027350 |
| Insert Size | 1677 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_027350.3, NP_081626.2 |
| RefSeq Size | 2660 bp |
| RefSeq ORF | 1677 bp |
| Locus ID | 70223 |
| UniProt ID | Q8BP47 |
| Gene Summary | Catalyzes the attachment of asparagine to tRNA(Asn) in a two-step reaction: asparagine is first activated by ATP to form Asn-AMP and then transferred to the acceptor end of tRNA(Asn). In addition to its essential role in protein synthesis, acts as a signaling molecule that induces immune response in a CCR3-dependent manner.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. The resulting isoform (2) is 1 aa shorter than isoform 1. |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG208707 | Nars (tGFP-tagged) - Mouse asparaginyl-tRNA synthetase (Nars) |
CNY 6,180.00 |
|
| MR208707 | Nars (Myc-DDK-tagged) - Mouse asparaginyl-tRNA synthetase (Nars), transcript variant 2 |
CNY 5,610.00 |
|
| MR208707L3 | Lenti ORF clone of Nars (Myc-DDK-tagged) - Mouse asparaginyl-tRNA synthetase (Nars), transcript variant 2 |
CNY 7,510.00 |
|
| MR208707L4 | Lenti ORF clone of Nars (mGFP-tagged) - Mouse asparaginyl-tRNA synthetase (Nars), transcript variant 2 |
CNY 7,510.00 |
