Krt10 (NM_010660) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	D130054E02Rik; K1C1; K10; Krt-1.10; Krt1-10
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC218948 representing NM_010660 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGTTCTATACAGCTCCAGCAGCAAGCAGTTCTCTTCCTCCCGCAGTGGAGGAGGAGGCGGTGGTG GATCCGTCAGGGTTTCCAGCACCAGGGGCTCTCTTGGTGGAGGGTATAGCTCAGGAGGCTTCAGCGGTGG CTCTTTTAGCCGTGGGAGCTCTGGGGGAGGTTGCTTTGGGGGCTCATCAGGTGGTTACGGAGGTTTTGGA GGAGGAGGCAGCTTTGGGGGAGGCTATGGAGGAAGCAGCTTTGGGGGAGGCTATGGAGGTAGCAGTTTTG GGGGTGGCAGCTTCGGTGGAGGTGGCAGCTTCGGTGGAGGCAGCTTTGGTGGCGGTAGCTATGGAGGAGG CTTTGGCGGTGGTGGATTCGGAGGAGATGGTGGCAGCCTTCTCTCCGGAAACGAGAAGGTGACCATGCAG AACCTGAACGACCGCCTGGCCTCCTACATGGACAAAGTCCGGGCTCTGGAAGAGTCAAACTACGAGCTGG AGGGTAAAATCAAGGAGTGGTACGAGAAGCATGGCAACTCAAGCCAGCGAGAGCCCCGGGACTACAGCAA ATACTACAAAACCATCGAGGACCTTAAGGGGCAGATCCTCACCCTGACAACTGACAATGCCAACGTGCTG CTGCAGATTGACAATGCCCGCCTGGCAGCTGATGACTTCAGGCTGAAATACGAGAATGAGGTGACCCTGC GCCAGAGCGTGGAGGCCGACATCAATGGCCTGCGCAGGGTACTGGATGAGCTGACCCTTAGCAAGTCTGA CCTGGAAATGCAGATCGAAAGTCTGAACGAAGAGCTGGCCTACCTAAAGAAGAACCACGAAGAGGAAATG AGAGACCTTCAAAATGTGTCCACTGGTGATGTGAATGTGGAAATGAACGCTGCCCCAGGGGTTGACCTGA CTCAGCTGCTGAACAACATGAGAAACCAATACGAACAACTTGCAGAAAAGAATCGCAAGGATGCTGAAGA GTGGTTCAATCAGAAGAGCAAGGAACTCACCACAGAAATCGACAGCAACATTGAACAAATGTCCAGCCAT AAGTCTGAAATCACTGAATTGAGACGTACTGTTCAGGGTCTGGAGATTGAACTACAGTCCCAGCTGGCCC TGAAACAATCGCTGGAAGCCTCCTTGGCAGAAACAGAAGGTCGCTACTGCGTGCAGCTCTCCCAGATTCA AAGCCAGATCTCCGCCCTGGAGGAACAGCTGCAACAGATTCGGGCTGAGACCGAGTGCCAGAACGCCGAG TACCAACAACTCCTAGACATCAAGACCCGACTGGAGAACGAGATTCAGACCTACCGCAGCCTGCTAGAAG GAGAGGGAAGCTCCAGCGGTGGCGGCGGCGGACGCGGCGGCGGCAGCCACGGCGGTAGCTACGGCGGAAG CTCCGGTGGCGGAAGCTACGGCGGAAGTTCCGGCGGCGGCGGCAGCTACGGCGGAAGTTCCGGCGGCGGC GGCAGCTACGGCGGCGGAAGCTCCGGGGGCGGTAGCCACGGAGGCAGTTCCGGTGGCGGCTACGGCGGCG GAAGTTCCAGCGGTGGTGCTGGTGGTCACGGCGGCAGTTCCGGTGGCGGCTACGGCGGCGGAAGTTCCAG CGGAGGCCAGGGCGGAAGCGGAGGTTTCAAGTCTTCCGGTGGCGGCGACCAATCATCTAAAGGACCAAGA TACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_010660
Insert Size	1686 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_010660.2, NP_034790.2
RefSeq Size	2124 bp
RefSeq ORF	1686 bp
Locus ID	16661
UniProt ID	P02535
Gene Summary	Plays a role in the establishment of the epidermal barrier on plantar skin.[UniProtKB/Swiss-Prot Function]

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