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Il10ra (NM_008348) Mouse Untagged Clone
CAT＃: MC219161
Il10ra (untagged) - Mouse interleukin 10 receptor, alpha (Il10ra), (10ug)
CNY 4,288.00
CNY 7,320.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AW553859; CDw210; CDw210a; IL-10R1; IL-10RA; Il10r; mIL-10R |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC219161 representing NM_008348
Red=Cloning site Blue=ORF TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGTCGCGTTTGCTCCCATTCCTCGTCACGATCTCCAGCCTGAGCCTAGAATTCATTGCATACGGGA CAGAACTGCCAAGCCCTTCCTATGTGTGGTTTGAAGCCAGATTTTTCCAGCACATCCTCCACTGGAAACC TATCCCAAACCAGTCTGAGAGCACCTACTATGAAGTGGCCCTCAAACAGTACGGAAACTCAACCTGGAAT GACATCCATATCTGTAGAAAGGCTCAGGCATTGTCCTGTGATCTCACAACGTTCACCCTGGATCTGTATC ACCGAAGCTATGGCTACCGGGCCAGAGTCCGGGCAGTGGACAACAGTCAGTACTCCAACTGGACCACCAC TGAGACTCGCTTCACAGTGGATGAAGTGATTCTGACAGTGGATAGCGTGACTCTGAAAGCAATGGACGGC ATCATCTATGGGACAATCCATCCCCCCAGGCCCACGATAACCCCTGCAGGGGATGAGTACGAACAAGTCT TCAAGGATCTCCGAGTTTACAAGATTTCCATCCGGAAGTTCTCAGAACTAAAGAATGCAACCAAGAGAGT GAAACAGGAAACCTTCACCCTCACGGTCCCCATAGGGGTGAGAAAGTTTTGTGTCAAGGTGCTGCCCCGC TTGGAATCCCGAATTAACAAGGCAGAGTGGTCGGAGGAGCAGTGTTTACTTATCACGACGGAGCAGTATT TCACTGTGACCAACCTGAGCATCTTAGTCATATCTATGCTGCTATTCTGTGGAATCCTGGTCTGTCTGGT TCTCCAGTGGTACATCCGGCACCCGGGGAAGTTGCCTACAGTCCTGGTCTTCAAGAAGCCTCACGACTTC TTCCCAGCCAACCCTCTCTGCCCAGAAACTCCCGATGCCATTCACATCGTGGACCTGGAGGTTTTCCCAA AGGTGTCACTAGAGCTGAGAGACTCAGTCCTGCATGGCAGCACCGACAGTGGCTTTGGCAGTGGTAAACC ATCACTTCAGACTGAAGAGTCCCAATTCCTCCTCCCTGGCTCCCACCCCCAGATACAGGGGACTCTGGGA AAAGAAGAGTCTCCAGGGCTACAGGCCACCTGTGGGGACAACACGGACAGTGGGATCTGCCTGCAGGAGC CCGGCTTACACTCCAGCATGGGGCCCGCCTGGAAGCAGCAGCTTGGATATACCCATCAGGACCAGGATGA CAGTGACGTTAACCTAGTCCAGAACTCTCCAGGGCAGCCTAAGTACACACAGGATGCATCTGCCTTGGGC CATGTCTGTCTCCTAGAACCTAAAGCCCCTGAGGAGAAAGACCAAGTCATGGTGACATTCCAGGGCTACC AGAAACAGACCAGATGGAAGGCAGAGGCAGCAGGCCCAGCAGAATGCTTGGACGAAGAGATTCCCTTGAC AGATGCCTTTGATCCTGAACTTGGGGTACACCTGCAGGATGATTTGGCTTGGCCTCCACCAGCTCTGGCC GCAGGTTATTTGAAACAGGAGTCTCAAGGGATGGCTTCTGCTCCACCAGGGACACCAAGTAGACAGTGGA ATCAACTGACCGAAGAGTGGTCACTCCTGGGTGTGGTTAGCTGTGAAGATCTAAGCATAGAAAGTTGGAG GTTTGCCCATAAACTTGACCCTCTGGACTGTGGGGCAGCCCCTGGTGGCCTCCTGGATAGCCTTGGCTCT AACCTGGTCACCCTGCCGTTGATCTCCAGCCTGCAGGTAGAAGAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_008348 |
| Insert Size | 1728 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_008348.2, NP_032374.1 |
| RefSeq Size | 3493 bp |
| RefSeq ORF | 1728 bp |
| Locus ID | 16154 |
| UniProt ID | Q61727 |
| Gene Summary | Cell surface receptor for the cytokine IL10 that participates in IL10-mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Upon binding to IL10, induces a conformational change in IL10RB, allowing IL10RB to bind IL10 as well. In turn, the heterotetrameric assembly complex, composed of two subunits of IL10RA and IL10RB, activates the kinases JAK1 and TYK2 that are constitutively associated with IL10RA and IL10RB respectively. These kinases then phosphorylate specific tyrosine residues in the intracellular domain in IL10RA leading to the recruitment and subsequent phosphorylation of STAT3 (PubMed:8910398). Once phosphorylated, STAT3 homodimerizes, translocates to the nucleus and activates the expression of anti-inflammatory genes. In addition, IL10RA-mediated activation of STAT3 inhibits starvation-induced autophagy (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longer isoform (1). |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG219426 | Il10ra (tGFP-tagged) - Mouse interleukin 10 receptor alpha (Il10ra), (10ug) |
CNY 5,510.00 |
|
| MR219426 | Il10ra (Myc-DDK-tagged) - Mouse interleukin 10 receptor, alpha (Il10ra) |
CNY 4,280.00 |
|
| MR219426L3 | Lenti ORF clone of Il10ra (Myc-DDK-tagged) - Mouse interleukin 10 receptor, alpha (Il10ra) |
CNY 6,940.00 |
|
| MR219426L4 | Lenti ORF clone of Il10ra (mGFP-tagged) - Mouse interleukin 10 receptor, alpha (Il10ra) |
CNY 6,940.00 |
