Fzd10 (NM_175284) Mouse Untagged Clone

CAT#: MC219323

Fzd10 (untagged) - Mouse frizzled homolog 10 (Drosophila) (Fzd10), (10ug)



  "NM_175284" in other vectors (4)

CNY 7,410.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Fz-10
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC219323 representing NM_175284
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAACACCCGGGCCCGCGCCTGTGGCTGGTGCTGCAGGTGATGATAGGCTCGTGCACGGCCATCAGCT
CCATGGACTTAGAGCGCCCTGGAGACGGCAAGTGCCAGCCGGTGGAGATTCCCATGTGCAAGGACATCGG
CTACAACACCACCCGCATGCCCAACCTGATGGGTCACGAGAACCAGCGCGAGGCGGCCATCCAACTGCAC
GAGTTCGCGCCGCTCGTGGAGTACGGCTGCCACAGCCACCTTCGCTTCTTCCTGTGTTCGCTGTACGCGC
CCATGTGCACCGAGCAGGTCTCCACACCCATCCCTGCTTGCCGGGTCATGTGCGAGCAGGCCCGGCTCAA
GTGCTCGCCGATCATGGAGCAGTTCAAATTCAGGTGGCCGGACTCCCTGGATTGCAGCAAGCTCCCCAAC
AAGAACGACCCCAACTACCTGTGCATGGAGGCACCCAACAACGGCTCGGATGAGCCCAGCCGGGGCTCTG
GCATGTTTCCTCCGCTCTTCAGGCCCCAGAGGCCCCACAGCGCGCAGGAGCACCCACTAAAGGACGGGGG
TCCGGGGCGCGCAGGTTGTGACAACCCAGGCAAGTTCCACCATGTGGAGAAGAGCGAATCTTGCGCACCG
CTTTGCACTCCGGGGGTGGATGTGTATTGGAGCCGCGACGACAAGCGCTTCGCTGTGGTCTGGCTGGCCA
TCTGGTCCGTGCTGTGCTTCTTCTCCAGCGCCTTCACCGTGCTCACCTTCCTCATCGACCCATCGCGCTT
CAGGTACCCCGAACGTCCTATCATCTTCCTCTCCATGTGCTACTGCGTTTATTCGGTGGGCTATATCATC
CGCCTCTTCGCGGGCGCGGAGAGCATCGCTTGTGACCGGGACAGTGGGCAGCTGTATGTTATCCAGGAAG
GACTGGAGAGCACGGGCTGTACCTTAGTCTTCTTGGTACTTTACTACTTCGGCATGGCCAGCTCTTTATG
GTGGGTGGTCCTCACCCTCACTTGGTTCCTGGCTGCTGGAAAGAAGTGGGGCCATGAGGCCATTGAAGCC
AACAGCAGCTACTTTCACCTGGCAGCTTGGGCCATCCCGGCTGTGAAGACTATCTTGATCTTGGTGATGC
GCAGGGTGGCAGGGGATGAGCTCACTGGTGTGTGTTATGTGGGCAGCATGGATGTCAATGCTCTGACCGG
CTTCGTGCTGGTCCCGCTGGCTTGCTACCTAGTCATCGGCACTTCCTTCATCCTGTCCGGCTTTGTGGCT
TTATTCCACATCCGGAGGGTGATGAAAACGGGTGGGGAGAACACGGACAAGCTGGAGAAGCTCATGGTAC
GCATAGGGGTCTTCTCCCTCCTCTACACTGTGCCGGCCACCTGTGTGATTGCCTGCTATTTTTATGAACG
CCTCAACATGGACTACTGGAAGATGCTGGCCACCCAGCACAAGTGTAAGATGAACAATCAGACCAAGACA
CCTGACTGCCTGATGACCACCTCCATCCCTGCCGTGGAGGTCTTCATGGTCAAAGTGTCCATGCTGCTGG
TGGTGGGCATCACCAGTGGGGTGTGGGTCTGGACTTCCAAGACCCTGCAGTCCTGGCAACACGTATGCAG
CCGGGGGCTAAAGAGAAAAAGCCGGAGGAAACCAGCCAGTGTGGTCACCAGTGCAGGGATCTACAAAAAA
GCCCAGCACCCCCAAAAACCTCACCTTGGGAAGTATGAACTGCCCGCCCAGCCTTCAGCCTGCGTGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_175284
Insert Size 1749 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_175284.3, NP_780493.1
RefSeq Size 3005 bp
RefSeq ORF 1749 bp
Locus ID 93897
UniProt ID Q8BKG4
Gene Summary Receptor for Wnt proteins (PubMed:15923619). Functions in the canonical Wnt/beta-catenin signaling pathway (PubMed:15923619). The canonical Wnt/beta-catenin signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues (Probable).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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