Mcoln1 (NM_053177) Mouse Untagged Clone

CAT#: MC219324

Mcoln1 (untagged) - Mouse mucolipin 1 (Mcoln1), (10ug)



  "NM_053177" in other vectors (4)

CNY 6,488.00


货期*
现货

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (3)
Rabbit Polyclonal Anti-TRPML1
    • 50 ul

CNY 11,000.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 2210015I05Rik; mucolipidin; TRPML1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC219324 representing NM_053177
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCACCCCGGCGGGCCGGCGCGCCTCAGAGACTGAGCGGCTGCTGACCCCCAATCCTGGGTATGGGA
CCCAGGTGGGGACCTCACCAGCCCCAACAACCCCCACAGAAGAGGAAGACCTCCGCCGCCGCCTCAAGTA
CTTCTTTATGAGCCCATGTGACAAGTTCCGGGCCAAAGGCCGCAAGCCCTGCAAGCTGATGCTGCAGGTG
GTCAAGATCTTGGTGGTCACTGTGCAGCTCATTCTCTTTGGGCTCAGCAACCAGCTGGTGGTGACATTCC
GGGAAGAGAACACCATTGCCTTCCGACATCTCTTCCTGCTGGGTTACTCTGATGGGTCTGATGACACCTT
TGCAGCCTACACACAGGAGCAGCTCTACCAAGCCATCTTCTATGCTGTGGACCAGTACCTGATACTACCT
GAGATATCCCTGGGCCGGTATGCCTATGTCCGTGGTGGGGGTGGGCCTTGGGCCAATGGATCAGCTTTGG
CTCTCTGCCAGCGGTACTACCACCGTGGCCATGTGGACCCAGCCAATGATACCTTTGACATTGATCCAAG
GGTAGTCACTGACTGTATCCAGGTGGATCCTCCTGACAGACCCCCTGACATCCCCAGTGAGGACTTGGAC
TTCTTGGATGGCAGCGCCAGTTACAAGAACCTCACACTGAAATTCCACAAGCTGATCAACGTCACCATCC
ACTTCCAGCTGAAGACAATTAACCTGCAGAGCCTCATCAACAATGAGATCCCTGATTGTTACACCTTCAG
TATCCTGATCACATTTGACAATAAAGCGCACAGTGGGCGAATCCCCATCCGCCTGGAGACCAAGACCCAC
ATCCAGGAGTGCAAACACCCCAGTGTCTCCAGACATGGAGACAACAGCTTCCGGCTTCTGTTTGATGTGG
TGGTTATCCTCACCTGCTCCCTGTCCTTCCTGCTGTGCGCCCGCTCACTGCTCCGTGGCTTCCTGCTGCA
GAACGAGTTTGTTGTATTCATGTGGCGGCGGCGGGGTCGGGAAATCAGCCTCTGGGAACGGCTGGAGTTT
GTCAATGGCTGGTACATCCTGCTGGTCACCAGTGACGTGCTCACCATCTCGGGGACTGTCATGAAGATTG
GCATTGAGGCAAAGAACCTAGCCAGCTATGATGTCTGCAGTATTCTCTTGGGTACCTCCACTCTGCTAGT
CTGGGTCGGTGTCATTCGCTACCTGACATTTTTCCACAAGTACAACATCTTGATTGCCACGTTGCGAGTG
GCACTGCCCAGTGTCATGCGTTTCTGCTGCTGTGTGGCTGTCATCTACCTGGGCTATTGCTTCTGTGGCT
GGATCGTTCTAGGGCCCTACCATGTGAAGTTCCGCTCGCTGTCCATGGTTTCTGAGTGTCTGTTCTCACT
CATCAACGGAGACGACATGTTTGTGACGTTCGCGGCCATGCAGGCCCAGCAGGGTCACAGCAGCCTGGTG
TGGCTCTTCTCCCAGCTGTACCTCTACTCCTTCATCAGCCTCTTCATCTACATGGTGCTGAGCCTCTTCA
TTGCACTCATCACCGGCGCCTATGACACCATCAAGCACCCAGGAGGTACTGGCACAGAGAAGAGTGAGCT
CCAGGCCTACATCGAGCAGTGCCAGGATAGCCCCACATCTGGCAAGTTCCGTCGTGGCAGTGGCTCAGCT
TGTAGCCTTTTCTGCTGCTGTGGAAGGGACTCCCCGGAGGACCATTCGCTGCTGGTGAACTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_053177
Insert Size 1743 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_053177.1, NP_444407.1
RefSeq Size 2039 bp
RefSeq ORF 1743 bp
Locus ID 94178
UniProt ID Q99J21
Gene Summary Nonselective cation channel probably playing a role in the regulation of membrane trafficking events and of metal homeostasis (PubMed:29019981). Proposed to play a major role in Ca(2+) release from late endosome and lysosome vesicles to the cytoplasm, which is important for many lysosome-dependent cellular events, including the fusion and trafficking of these organelles, exocytosis and autophagy. Required for efficient uptake of large particles in macrophages in which Ca(2+) release from the lysosomes triggers lysosomal exocytosis. May also play a role in phagosome-lysosome fusion (PubMed:23993788). Involved in lactosylceramide trafficking indicative for a role in the regulation of late endocytic membrane fusion/fission events. By mediating lysosomal Ca(2+) release is involved in regulation of mTORC1 signaling and in mTOR/TFEB-dependent lysosomal adaptation to environmental cues such as nutrient levels (PubMed:25733853). Seems to act as lysosomal active oxygen species (ROS) sensor involved in ROS-induced TFEB activation and autophagy (By similarity). Functions as a Fe(2+) permeable channel in late endosomes and lysosomes. Proposed to play a role in zinc homeostasis probably implicating its association with TMEM163 (By similarity). In adaptive immunity, TRPML2 and TRPML1 may play redundant roles in the function of the specialized lysosomes of B cells (PubMed:17050035).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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