Top1mt (NM_028404) Mouse Untagged Clone
CAT#: MC219428
Top1mt (untagged) - Mouse DNA topoisomerase 1, mitochondrial (Top1mt), nuclear gene encoding mitochondrial protein, (10ug)
CNY 7,510.00
Product images
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | 2900052H09Rik |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC219428 representing NM_028404
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCTGCTGTGGCTCAGAGCCCTGTGCCGGCGTTTCCAGCATGTCCCCTGCCGCGTCCCTTCCTGTC AGGTGTCCCGGGTCGCGAAGGCGAACAGAGCCGGGTGGGAAGAAACGAGCAAGAGCAGTGTGAAGTGGAG GCAGCTGGAGCACAAGGGCCCGTACTTCACCCCAGCCTACGAGCCCCTTCCTGACGGAGTGCACTTCCTC TACGATGGCAAACCGCTGAAGCTGAGCTTGGCAGCGGAGGAGGTCGCTACCTTCTATGGGAAAATGCTGC ATCTTGAGTGTACGAGCAAGGAAGTCTTTCGGAGGAACTTCTTCAGTGACTGGCGGAAGGAGATGACACC TGAGGAGAGGAGGCTCATCACACACCTTGACAAATGTGACTTCACGGAGATCCATAAGCACTTCCTGGAA AGAGCTGAGGCCCGCAGGACCCTGCCCAGGGAGCAGAAGCAGAAGCTAAAGGAAGAGGCCGAGAAACTGC AGCAGGAGTTTGGCTATTGCGTTTTAGATGGCCACAGAGAAAAAATAGGCAATTTCAAGACGGAACCACC CGGCTTGTTCCGTGGCCGAGGTGACCATCCCAAGATGGGGATGTTGAAGAGGAGGGTCATGCCAGAGGAT GTGGTCATTAACTGCAGCAGGGATTCCAAGATCCCTGAGCCCCCAGCCGGTCACCAGTGGAAAGAGGTAC GCTCAGATAACACAGTCATGTGGCTGGCTGCCTGGGTGGAGCACATCCAGAACTCCTTCAAGTATGTCAT ACTGAACCCCAGCTCCAAGCCGAAGGGGGAGATGGATTGGCAGAAGTATGAGGTAGCGCGACGCTTGAAG GGGGTTGTGGACAAGATCCGTGCCCAGTATCAGGCTGACTGGAAGTCACCAGAAATGAAGAAGAGACAGC TGGCTGTCGCCCTTTATTTTATTGATAAGCTGGCACTGCGGACAGGCAATGAGAAGGAAGAGGGCGAGAC GGCCGACACCGTGGGCTGCTGCTCGCTCCGTGTGGAGCACATCCGACTGCACGCGCCCGCAGGTGGCCAG GAGCACGTTGTAGAGCTGGACTTCTTGGGAAAGGATTCCATCCGCTATAAGAACCACGTGACGGTGGAGA AGCTCGTGTTCCAGAACCTTCAGCACTTCATGGAAGACAAGGACCCTAGGGATGACCTCTTTGATGCACT GACCACTTCCAGCCTGAACAAGCACTTGCAGGACTTGATGGAAGGGCTGACAGCCAAGGTGTTCCGGACC TACAATGCCTCTGTCACTCTGCAGGAGCAGCTGCGGGTGCTGACCCGTGCGGAAGACAGCGTAACCTGCA AAGTCTTAGCTTACAACCGGGCGAACCGGGCTGTGGCAGTCCTCTGTAACCATCAGAGAGCAGTTCCCAA GACCTTCGAGAAGTCGATGCAGACACTTCAAAAAAAGATTGAGACAAAGAAGGCACAGGTGGCTGAGGCA CAGGTAGAGCTGCAGAAAGCAGAGACTGACCTGAGAATGAGAGGGGACAGCAAACCCAAAAGTTTCCTTC AGAAGCAGCGACGATTGTTAAAGCTGGAAGAACAGCTGGCCCGATTGTGCGCGAAGGCCACAGACAAGGA GGAGAATAAGCAGGTGGCCCTGGGCACCGCCAAGCTCAACTATCTGGACCCCAGGATCAGCATCGCCTGG TGTAAGAGATTTGGAGTGCCTGTGGAGAAGATCTACAACAAGACCCAGAGAGAGAGGTTTGCCTGGGCCT TCAACCAGGCAGGTGAAGACTTTGAATTCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_028404 |
Insert Size | 1782 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_028404.2, NP_082680.1 |
RefSeq Size | 2011 bp |
RefSeq ORF | 1782 bp |
Locus ID | 72960 |
UniProt ID | Q8R4U6 |
Gene Summary | Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).[UniProtKB/Swiss-Prot Function] |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG220397 | Top1mt (tGFP-tagged) - Mouse DNA topoisomerase 1 mitochondrial (Top1mt) nuclear gene encoding mitochondrial protein, (10ug) |
CNY 5,800.00 |
|
MR220397 | Top1mt (Myc-DDK-tagged) - Mouse DNA topoisomerase 1, mitochondrial (Top1mt), nuclear gene encoding mitochondrial protein |
CNY 4,424.00 |
|
MR220397L3 | Lenti ORF clone of Top1mt (Myc-DDK-tagged) - Mouse DNA topoisomerase 1, mitochondrial (Top1mt), nuclear gene encoding mitochondrial protein |
CNY 7,130.00 |
|
MR220397L4 | Lenti ORF clone of Top1mt (mGFP-tagged) - Mouse DNA topoisomerase 1, mitochondrial (Top1mt), nuclear gene encoding mitochondrial protein |
CNY 7,130.00 |