C8a (NM_146148) Mouse Untagged Clone
CAT#: MC219464
C8a (untagged) - Mouse complement component 8, alpha polypeptide (C8a), (10ug)
CNY 7,510.00
Product images
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC219464 representing NM_146148
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTGTGGTTGCTTTCTTTGGCTTGTCTTTGGTGGCATGGCACCCTGGGGTAACTGCACAAGAGAAGG TGAACCAGCGGGTCACCCGAGCTGTGACCCCGCAAGCAGTGTCCTGTCAGTTGAGTGACTGGTACAAATG GACAGATTGTTTTCCATGTCAGGATAAAAAGTACCGATACCGGAGCCTCTTGCAGCCAAGCAAGTTTGGA GGAACCATCTGCAGTGGTGATATCTGGGATGAGGCCAGCTGTGACAGCCCCACACCTTGTCTAAGGCAAG CACAGTGTGGACAGGATTTCCAGTGTAGAGAGACAGGTCGCTGCCTGAAACGCCACCTGGTGTGTAACGG AGATAACGACTGCCTGGATGGCTCTGATGAGTCCGACTGTGAAGATGTCAGGGTCACTGAGGATGATTGC CATCAGTATGAACCAATCCCAGGATCAGAAAGGGCAGCCTTGGGGTACAACATCCTGACTCAAGAGGAAG CTCAGAGTGTTTACGATGCTAAGTATTATGGGGGCCAGTGTGAAACTGTGTACAATGGAGACTGGAGAAA GCTGCGGTACGACCCCACCTGTGAGCGCCTCTACTATGGGGAGGATGAGAAATACTTTCGGAAGCCCTAC AACTTCCTTAAGTACCACTTTGAAGCCCTGGCGGATACTTCAATCTCCTCAGAGTTTTATGATGATGCGA ATGATCTTTTTTTTCACATAAAGAATGGCAAGTCCCACTCAGCTGGTGTAACCGTTGGCGTAGCCCCAGT AAAAAGTCCTGTCTCAATAGAGGTGACTGGATCCGGGTCCAAAGCATCTTCATTCCTGAACAAGTTAAAC AAGTATAATGAGAAGAGATATGGCTTCATGAGAGTGTCTACAAAGATACAGACTGCACAGTTTAAGATGA GAAGGAACAATATTGTGCTGGATGAAGGAATGCTGCAGTCATTAATGGAGCTCCCAGAGCAGTTCAACTA TGGCATGTACGCCAAGTTCATCAATGACTATGGCACCCACTACATCACCTCTGGAACCATGGGTGGCATT TATGAATATGTTATGGTGCTTGACAAAGAGAAAATGAAAACTGAAGGTACTACTGTGGATGAAGTCCAAA AATGCATTGGCGGTGGCATTGGCATAGGAATTAAGGATAGTACAATTGAAGGAGTAGGTATATCAGGAGA GTTTTGTGAAAACTCTGGAGATGGTGACCGTGACATAAGGAAGAAAATCACAGGAGTGGAGGACATCATT TCTCGGGTGCAAGGTGGAAGTTCTGTCTGGGGCAGTGTCTTGACACACAACAGCAGTGCCATTACATATC AGTCCTGGGGGAGATCGTTAAAGTATAACCCTGTTGTTATCGATTTTGAGATGCAGCCTATCTACCAGCT ACTGCGGCACACAAACTTGGGACCCCTGGAGACGAAACGTCAGAACCTGCGACGGGCTTTGGACCAGTAC CTGATGGAATTTAATGCCTGTCGTTGTGGGCCCTGCTTCAACAATGGGGAACCCATACTTGACGGCACCA ACTGCCGGTGTCAGTGTAGTATGGGCCGGCAGGGCCTGGCCTGTGAACGCACGGTGATAGAGGGGCTCAA AGACTTCAAAGCCGCTGGCCACTGGAGCTGCTGGAGTTCCTGGTCAGAGTGCAGAGGAGGTTCCCAGGAG AGGAGGAGACAGTGCAACAACCCACCACCCAAGAATGGAGGTACCCCATGCCTAGGGAGGAACCTGCAGA CCCAGGCCTGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_146148 |
Insert Size | 1764 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_146148.2, NP_666260.1 |
RefSeq Size | 3636 bp |
RefSeq ORF | 1764 bp |
Locus ID | 230558 |
UniProt ID | Q8K182 |
Gene Summary | This gene encodes the alpha subunit of complement component C8 that participates in the assembly of the complement membrane attack complex. The encoded preproprotein undergoes proteolytic processing to generate the alpha subunit, which associates with the beta and gamma subunits to form a trimeric complement component, C8. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing. This gene is located adjacent to the gene encoding the beta subunit. [provided by RefSeq, Oct 2015] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). |
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SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG209163 | C8a (tGFP-tagged) - Mouse complement component 8, alpha polypeptide (C8a) |
CNY 5,040.00 |
|
MR209163 | C8a (Myc-DDK-tagged) - Mouse complement component 8, alpha polypeptide (C8a) |
CNY 4,376.00 |
|
MR209163L3 | Lenti ORF clone of C8a (Myc-DDK-tagged) - Mouse complement component 8, alpha polypeptide (C8a) |
CNY 6,460.00 |
|
MR209163L4 | Lenti ORF clone of C8a (mGFP-tagged) - Mouse complement component 8, alpha polypeptide (C8a) |
CNY 6,460.00 |