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Grn (NM_008175) Mouse Untagged Clone
CAT＃: MC219499
Grn (untagged) - Mouse granulin (Grn), (10ug)
CNY 7,700.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | epithelin; Pgrn |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC219499 representing NM_008175
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTCCCAGGGAGCCCGGACCCCGACGCAGGCAGACCATGTGGGTCCTGATGAGCTGGCTGGCCTTCG CGGCAGGGCTGGTAGCCGGAACACAGTGTCCAGATGGGCAGTTCTGCCCTGTTGCCTGCTGCCTTGACCA GGGAGGAGCCAACTACAGCTGCTGTAACCCTCTTCTGGACACATGGCCTAGAATAACGAGCCATCATCTA GATGGCTCCTGCCAGACCCATGGCCACTGTCCTGCTGGCTATTCTTGTCTTCTCACTGTGTCTGGGACTT CCAGCTGCTGCCCGTTCTCTAAGGGTGTGTCTTGTGGTGATGGCTACCACTGCTGCCCCCAGGGCTTCCA CTGTAGTGCAGATGGGAAATCCTGCTTCCAGATGTCAGATAACCCCTTGGGTGCTGTCCAGTGTCCTGGG AGCCAGTTTGAATGTCCTGACTCTGCCACCTGCTGCATTATGGTTGATGGTTCGTGGGGATGTTGTCCCA TGCCCCAGGCCTCTTGCTGTGAAGACAGAGTGCATTGCTGTCCCCATGGGGCCTCCTGTGACCTGGTTCA CACACGATGCGTTTCACCCACGGGCACCCACACCCTACTAAAGAAGTTCCCTGCACAAAAGACCAACAGG GCAGTGTCTTTGCCTTTTTCTGTCGTGTGCCCTGATGCTAAGACCCAGTGTCCCGATGATTCTACCTGCT GTGAGCTACCCACTGGGAAGTATGGCTGCTGTCCAATGCCCAATGCCATCTGCTGTTCCGACCACCTGCA CTGCTGCCCCCAGGACACTGTATGTGACCTGATCCAGAGTAAGTGCCTATCCAAGAACTACACCACGGAT CTCCTGACCAAGCTGCCTGGATACCCAGTGAAGGAGGTGAAGTGCGACATGGAGGTGAGCTGCCCTGAAG GATATACCTGCTGCCGCCTCAACACTGGGGCCTGGGGCTGCTGTCCATTTGCCAAGGCCGTGTGTTGTGA GGATCACATTCATTGCTGCCCGGCAGGGTTTCAGTGTCACACAGAGAAAGGAACCTGCGAAATGGGTATC CTCCAAGTACCCTGGATGAAGAAGGTCATAGCCCCCCTCCGCCTGCCAGACCCACAGATCTTGAAGAGTG ATACACCTTGTGATGACTTCACTAGGTGTCCTACAAACAATACCTGCTGCAAACTCAATTCTGGGGACTG GGGCTGCTGTCCCATCCCAGAGGCTGTCTGCTGCTCAGACAACCAGCATTGCTGCCCTCAGGGCTTCACA TGTCTGGCTCAGGGGTACTGTCAGAAGGGAGACACAATGGTGGCTGGCCTGGAGAAGATACCTGCCCGCC AGACAACCCCGCTCCAAATTGGAGATATCGGTTGTGACCAGCATACCAGCTGCCCAGTAGGGCAAACCTG CTGCCCAAGCCTCAAGGGAAGTTGGGCCTGCTGCCAGCTGCCCCATGCTGTGTGCTGTGAGGACCGGCAG CACTGTTGCCCGGCCGGGTACACCTGCAATGTGAAGGCGAGGACCTGTGAGAAGGATGTCGATTTTATCC AGCCTCCCGTGCTCCTGACCCTCGGCCCTAAGGTTGGGAATGTGGAGTGTGGAGAAGGGCATTTCTGCCA TGATAACCAGACCTGTTGTAAAGACAGTGCAGGAGTCTGGGCCTGCTGTCCCTACCTAAAGGGTGTCTGC TGTAGAGATGGACGTCACTGTTGCCCCGGTGGCTTCCACTGTTCAGCCAGGGGAACCAAGTGTTTGCGAA AGAAGATTCCTCGCTGGGACATGTTTTTGAGGGATCCGGTCCCAAGACCGCTACTGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_008175 |
| Insert Size | 1809 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_008175.4, NP_032201.2 |
| RefSeq Size | 2348 bp |
| RefSeq ORF | 1809 bp |
| Locus ID | 14824 |
| UniProt ID | P28798 |
| Gene Summary | Secreted proteins that act as key regulator of lysosomal function and as a growth factor involved in inflammation, wound healing and cell proliferation (PubMed:28073925, PubMed:8496151, PubMed:28541286, PubMed:28453791, PubMed:20026663, PubMed:23041626, PubMed:27789271, PubMed:12524533). Functions as regulator of proteins trafficking to lysosome and activity of lysosomal enzymes (PubMed:28453791, PubMed:28541286, PubMed:27789271). Facilitates also the acidification of lysosomes, causing degradation of mature CTSD by CTSB (PubMed:28073925). In addition, functions as wound-related growth factor that acts directly on dermal fibroblasts and endothelial cells to promote division, migration and the formation of capillary-like tubule structure (PubMed:12524533). Also promotes epithelial cell proliferation by blocking TNF-mediated neutrophil activation preventing release of oxidants and proteases (PubMed:8496151). Moreover, modulates inflammation in neuron by preserving neuron survival, axonal outgrowth and neuronal integrity (PubMed:23041626, PubMed:20026663).[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG225358 | Grn (tGFP-tagged) - Mouse granulin (Grn), (10ug) |
CNY 8,320.00 |
|
| MR225358 | Grn (Myc-DDK-tagged) - Mouse granulin (Grn) |
CNY 6,720.00 |
|
| MR225358L3 | Lenti ORF clone of Grn (Myc-DDK-tagged) - Mouse granulin (Grn) |
CNY 7,890.00 |
|
| MR225358L4 | Lenti ORF clone of Grn (mGFP-tagged) - Mouse granulin (Grn) |
CNY 7,890.00 |
