United States
Germany
Japan
United Kingdom
China
Lmnb2 (NM_010722) Mouse Untagged Clone
CAT＃: MC219504
Lmnb2 (untagged) - Mouse lamin B2 (Lmnb2), (10ug)
CNY 7,700.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC219504 representing NM_010722
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTCTCTGCCGCCCCACGCGGGGCCCGCCACGCCGCTGTCGCCCACGCGTCTGTCTCGGCTGCAGG AGAAGGAGGAGCTCCGTGAGCTCAACGACCGCCTGGCACACTACATCGACCGTGTCCGCGCGCTGGAGCT GGAGAATGATAGGTTGCTGCTCCGGATCTCCGAGAAGGAGGAGGTGACCACTCGCGAGGTGAGTGGCATC AAGACCCTGTACGAGTCAGAGCTGGCTGACGCCCGACGGGTACTGGATGAGACGGCCCGTGAACGTGCCC GGCTGCAGATTGAAATTGGAAAGGTGCAGGCTGAGCTAGAGGAGGCCAGGAAGAGTGCCAAGAAGCGGGA AGGTGAGCTCACAGTGGCCCAGGGCCGAGTGAAGGATCTGGAATCACTGTTTCACCGGAGTGAGGCTGAG CTGGCCACAGCCCTCAGTGACAAGCAGGGCCTGGAGACAGAGGTGGCAGAGCTTCGAGCACAGCTGGCCA AGGCAGAAGATGGTCATGCTGTGGCCAAGAAGCAGTTGGAGAAGGAGACGCTGATGCGTGTGGACCTGGA GAACCGATGCCAGAGCCTGCAGGAGGAGCTGGCTTTCAGCAAGAGTGTGTTTGAGGAGGAGGTACGGGAG ACCCGACGGAGGCACGAGCGGCGCTTGGTGGAGGTGGACAGCAGCCGGCAACAGGAATATGACTTCAAGA TGGCTCAGGCCCTGGAGGACCTGCGCAGTCAGCACGATGAGCAAGTGCGCCTGTACCGGGTAGAACTGGA GCAGACCTACCAGGCCAAGCTGGACAACGCCAAGCTGCTCTCGGACCAGAATGACAAGGCAGCCCATGCA GCCCGCGAGGAGCTCAAGGAGGCCCGCATGCGCGTGGAGTCCCTCAGCTACCAGCTTTTAGGCCTCCAAA AGCAGGCCAGTGCTGCAGAGAACCACATCCATGAGCTGGAGGAGGCCTTGGCTGGGGAGCGTGACAAGTT CCGCAAGATGCTGGACGCTAAGGAACAGGAGATGACGGAGGTGCGGGACGCCATGCAGCAGCAGCTGGCA GAGTACCAAGAGCTGCTGGACATTAAGCTGGCCCTGGACATGGAGATAAGCGCCTACCGCAAGCTGCTGG AGGGCGAGGAGGAGAGGCTGAAGCTGTCTCCCAGCCCTTCATCACGGATCACCATCTCTCGGGCCACATC GAGCAGCAGCAGCAGCAGCGGGGTTGGCATGTCTGTGGGCCAGGGCCGGGGCAAGCGCCGGCGGCTGGAG ACTGAGGACACCTCAGGCTCACCCAGCAGGGCTTCCAGAGTGAGCAGCGGCTCCCGCCTGGCTCAGCAGA CTGTGGCCACGGGTGTTGTGAACATCGACGAGGTGGACCCAGAGGGCAGGTTCGTGCGCCTTAAGAACTC TTCAGACAAGGACCAGTCTTTGGGGAACTGGAGGATCAAGAGACAGGTTCTGGAGGGTGAGGACATTGCC TACAAGTTCACACCCAAGTATGTCCTGCGGGCCGGCCAGACTGTCACGGTGTGGGCAGCTGGCGCAGGGG CTACCCACAGTCCCCCATCAACCCTTGTGTGGAAAAGCCAGACCAACTGGGGCCCTGGGGAGAGCTTCCG CACTGCCCTGGTCAGTGCCGACGGTGAGGAGGTGGCCGTGAAGGCTGCAAAGCACTCATCTGTCCAGGGG AGGGAGAACGGGGAAGAGGAGGAAGAGGAAGAGGCAGAATTCGGTGAAGAGGACCTCTTCCACCAGCAGG GGGACCCAAGGACTACCTCAAGGGGCTGCCGACTGATGTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-RsrII |
| ACCN | NM_010722 |
| Insert Size | 1791 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_010722.5, NP_034852.3 |
| RefSeq Size | 3389 bp |
| RefSeq ORF | 1848 bp |
| Locus ID | 16907 |
| UniProt ID | P21619 |
| Gene Summary | This gene encodes a protein component of the nuclear lamina, which provides a structural framework for the nuclear envelope. Defects in this gene were found to cause abnormalities in the shape of neurons. This locus represents one of two B-type lamin genes that may be partially, but not entirely, functionally redundant in neuronal development. Loss of both B-type lamin genes in keratinocytes results in ichthyosis and a skin barrier defect leading to dehydration. Alternative transcriptional initiation and splicing results in multiple transcript variants and protein isoforms, including an isoform with a shorter N-terminal rod domain that may function in nuclear envelope remodeling during spermatogenesis. A related pseudogene is found on chromosome 5. [provided by RefSeq, Sep 2017] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: An in-frame AUG is located 19 codons upstream of the annotated translation start site but is not being annotated as a start site since it is in a weak Kozak sequence context and is probably too close to the 5' end of the transcript to be used as a translation start site. CCDS Note: The coding region has been updated to start at an alternate in-frame start codon that is more supported by available transcript data. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR209436 | Lmnb2 (Myc-DDK-tagged) - Mouse lamin B2 (Lmnb2) |
CNY 4,440.00 |
|
| MR209436L3 | Lenti ORF clone of Lmnb2 (Myc-DDK-tagged) - Mouse lamin B2 (Lmnb2) |
CNY 6,460.00 |
|
| MR209436L4 | Lenti ORF clone of Lmnb2 (mGFP-tagged) - Mouse lamin B2 (Lmnb2) |
CNY 6,460.00 |
