Naf1 (NM_001163564) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Gm174
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC219579 representing NM_001163564 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGTGGTGGAGGCCGCCGCGCAGCTGCAGACGCTCAAGTTCGGCGGCTCCGGCCAGGGGTCGGCAG CACCGCAGCCACCTGAGGATCGCCGGGAGGCCCCGCCGCCGGGGGTGCAGCCGCCGCCGCCAGCCCCGAG CTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAGCTCGGATCCCGGCGGAAGACCACCGCCG CTGCCAGCCCCGAACTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAGCTCGGATCCCGGCG GAAGACCACCGCCGCTGCCAGCCCCGAACTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAG CTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAACTCGGATCCCGGCGGAAGACCACCGCCG CCGCCAGCCCCGAGCTCGGATCCCGGCGGAAGACCACCGCCGCCGCCAGCCCCGAACTGGGATGCGGGCG GAAGACCACCACCGCCGCCAGCCCCGAACTCGGATCCCGGCGGAAGACCGACTCCGCCGCTGCCAGCCCT GAACTGGGATGCGGGCGGAAGACCACCGCCGCCGGGCGGGGCCTGGGTCACCGGCCGGAACGCCGCGGAG CCGCCGCCAGTGCTGCAGGCCTCGGACTCCTCGGACTCGGATTCGGACAGTGAAACAGATTCAGATAGCT CCAGCTCCTCCTCTTCGTCGTCCTCTTCATCTTCATCCTGTGTATCATTTCCTCCTGTGTTGTCAGATGG AGATGAGGATTTTCAACTTGAGAAAGAAAATAAGAATTTTCCTCTTAAAACAAAAGATGAATTGCTTCTT AATGAACTACCTTCTGTTGAAGAACTCACTGTAATTCTGCCTGAAGACATTGCACTAAAGCCTCTTGGGA AGGTTTCAAGCATTATTGAACAATTAGTAATAATTGAGTCTGTGACTAACATACCTCCAGTTAATGAGGA TACTGTCATTTTCAAAAGTGATCGACAAGCAGCAGGGAAGATATTTGAGATATTTGGACCTGTTGCCCAT CCATTTTATGTGTTACGGTTTAATTCATCAGATCACATTGAGAGTAAAGGTATCAAAATAAATGACACGA TGTATTTTGCTCCATCAATGAAAGACTTCACTCAATATATATTCACAGAAAAGCTCAAACAGGACAGAGG ATCGGACGCTTCTTGGAAGAATGATCAGGAACCCCCTCCAGAAGTCTTGGACTTCAGTGACGATGAGAAA GAAAAAGAAGCCAAACAGAGAAAAAAATCTCAGATTCAAGGCCGCAAAAAACTCAAATCTGAATTGAATG AGTCAGGTGAAGATTTTGGTGAAGTGCATGAGAATTGGAATGCTTATAGCTCTTCAGAACATTCAAAAGG ATATCACCACAGGGAATTCTCAAGAGGCTTTGCGAGGGGTAGATACTCTCGAAGAAGCCATGGCAGGCCT CCACCTCAGCAGTACTATAACTCAGACCATATGGCATCTCAAGAGAGTTTGGGATTTACACCTCAGAGAC AAGATAATCCTGTCATGCCCCACTATCCTTTTCCCCCGCCCATGTTTGATATGCATAACTTTCCACTTCC GCCCCCACCCCCACCCCCGCCTCCGCCCCCACCAAGCATGGGGTGGGCTGCACCTAGCATGGCCTCTCAT CCTGTGCTTAACTTACCATACTCCATGCCCCCACCACCTCTTCCTCCACCACCTCCACCTCCCCCTCCCT CTCCTGGGGAAAATAATTCTTCTCATTTTGGGCCTTATTTTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001163564
Insert Size	1794 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001163564.1, NP_001157036.1
RefSeq Size	2990 bp
RefSeq ORF	1794 bp
Locus ID	234344
UniProt ID	Q3UMQ8
Gene Summary	RNA-binding protein required for the maturation of box H/ACA snoRNPs complex and ribosome biogenesis. During assembly of the H/ACA snoRNPs complex, it associates with the complex and disappears during maturation of the complex and is replaced by NOLA1/GAR1 to yield mature H/ACA snoRNPs complex. Probably competes with NOLA1/GAR1 for binding with DKC1/NOLA4 (By similarity).[UniProtKB/Swiss-Prot Function]

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