Aifm1 (NM_012019) Mouse Untagged Clone

CAT#: MC219720

Aifm1 (untagged) - Mouse apoptosis-inducing factor, mitochondrion-associated 1 (Aifm1), nuclear gene encoding mitochondrial protein, (10ug)



  "NM_012019" in other vectors (4)

CNY 7,890.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AIF; AIFsh2; Hq; Pdcd8
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC219720 representing NM_012019
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTCCGGTGTGGAGGCCTGGCGGGTGCTTTCAAGCAGAAACTGGTGCCCTTGGTGCGGACGGTGTACG
TCCAGAGGCCGAAACAGAGGAACCGGCTTCCAGGCAACTTGTTCCAGCAATGGCGTGTTCCTCTAGAACT
CCAGATGGCAAGACAAATGGCTAGCTCTGGTTCATCAGGGGGCAAAATGGATAATTCTGTGTTAGTCCTT
ATTGTGGGCTTATCAACAATAGGAGCTGGTGCATATGCCTACAAAACTATAAAAGAAGACCAAAAAAGAT
ACAATGAAAGAGTGATGGGATTAGGACTGTCCCCAGAAGAGAAACAGAGAAGAGCCATTGCCTCCGCTAC
AGAGGGAGGCTCAGTTCCTCAGATCAGGGCACCAAGTCACGTCCCTTTCCTGCTGATTGGTGGAGGGACT
GCTGCTTTTGCAGCAGCCAGATCCATCCGGGCTCGGGATCCTGGGGCCAGGGTCCTGATTGTATCTGAAG
ACCCTGAACTGCCATACATGCGACCTCCTCTTTCAAAAGAATTGTGGTTTTCAGATGATCCAAATGTCAC
AAAGACACTGCAATTCAGACAGTGGAATGGAAAAGAGAGAAGCATATATTTCCAGCCACCTTCTTTCTAT
GTCTCTGCTCAGGACCTGCCTAATATTGAGAACGGTGGTGTGGCTGTCCTCACTGGGAAAAAGGTAGTAC
ATCTGGATGTAAGAGGCAACATGGTGAAACTTAATGATGGCTCTCAGATTACCTTTGAAAAGTGCTTGAT
TGCAACGGGAGGCACTCCAAGAAGTCTGTCTGCCATCGATAGGGCTGGAGCAGAGGTGAAGAGTAGAACA
ACACTTTTCAGGAAGATTGGAGATTTTAGAGCCTTGGAGAAGATCTCTCGGGAGGTCAAGTCAATTACAG
TTATCGGCGGGGGCTTCCTTGGGAGTGAGCTGGCCTGTGCTCTTGGCAGAAAGTCTCAAGCCTCGGGCAT
AGAAGTGATCCAGCTGTTCCCTGAGAAAGGAAATATGGGGAAGATCCTTCCTCAATACCTCAGCAACTGG
ACCATGGAAAAAGTCAAACGAGAGGGAGTGAAAGTGATGCCCAATGCAATTGTACAATCAGTTGGAGTCA
GCGGTGGCAGGTTACTCATTAAGCTGAAAGATGGAAGGAAGGTAGAAACTGACCACATAGTGACAGCTGT
GGGCCTAGAGCCCAATGTTGAGTTGGCCAAGACTGGCGGACTGGAAATAGATTCCGATTTTGGTGGCTTC
CGGGTAAATGCAGAACTCCAAGCACGTTCTAACATCTGGGTGGCAGGGGATGCTGCATGCTTCTATGATA
TAAAGTTGGGTCGAAGGCGAGTAGAGCATCATGATCATGCTGTTGTGAGTGGAAGACTGGCTGGAGAAAA
CATGACTGGAGCCGCTAAGCCATACTGGCATCAGTCAATGTTCTGGAGTGATTTGGGTCCTGATGTCGGC
TATGAAGCTATTGGTCTGGTGGATAGTAGTTTGCCCACAGTTGGTGTTTTTGCAAAAGCAACTGCACAAG
ACAACCCAAAATCTGCCACAGAGCAGTCAGGAACTGGTATCCGTTCGGAGAGTGAGACAGAGTCAGAAGC
TTCGGAAATCACAATTCCTCCCAGCGCCCCTGCAGTCCCACAGGTCCCTGTTGAAGGGGAGGACTACGGC
AAAGGTGTCATCTTCTACCTCAGGGACAAAGTTGTGGTGGGGATTGTGCTATGGAACGTCTTTAACCGAA
TGCCAATTGCAAGGAAGATCATTAAGGACGGTGAGCAACATGAAGATCTCAATGAAGTAGCTAAACTCTT
CAACATTCATGAAGATTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_012019
Insert Size 1839 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_012019.3, NP_036149.1
RefSeq Size 2249 bp
RefSeq ORF 1839 bp
Locus ID 26926
UniProt ID Q9Z0X1
Gene Summary Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G, and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner (By similarity).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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