Ncbp3 (NM_025818) Mouse Untagged Clone

CAT#: MC219729

1200014J11Rik (untagged) - Mouse RIKEN cDNA 1200014J11 gene (1200014J11Rik), (10ug)



  "NM_025818" in other vectors (4)

CNY 7,890.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 1200014J11Rik; C78393; C130061O14Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC219729 representing NM_025818
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCCGTCCGCGGCCTGCGAGTGTCTGTGAAGGCGGAGGCCCCCGCTGGGCCGGCTTTGGGGCTTC
CGTCGCCCGAGGTGGAGTCCGGTTTGGAGCGTGGCGAGCCGGAGCCCATGGAAGTGGAAGAAGGCGAGCT
GGAAATCGTGCCGGTGCGGCGCTCCCTAAAGGAACTGCTCCCGGACACAAGCAGAAGATATGAAAACAAG
GCTGGCAGCTTCATCACTGGGATCGATGTCACCTCCAAGGAAGCAATTGAAAAGAAAGAACAGCGAGCCA
AGCGCTTCCACTTTCGAGCAGAAGTAAATCTTGCCCAGAGAAATGTAGCCTTGGACCGAGACATGATGAA
GAAAGCAATTCCCAAGGTGAGACTGGAGACAATCTACATTTGTGGAGTGGATGAAATGAGCACCCAGGAT
ATCTTCTCCTATTTTAAGGAATATCCTCCAGCTCACATTGAGTGGTTGGATGACACCTCCTGCAATGTGG
TTTGGCTGGACGAGATGACGGCCACACGAGCTCTCATCAATATGAGTTCTCTGCCAGCCCAGGATAAGAT
GAGAAGCAGAGATGCCAGTGAAGACAAGTCGTCGGAGAAGAACAAGAAAGACAAGCAGGAAGACAGTTCA
GATGACGACGAGACTGAAGAAGGAGAAGTGGAAGATGAGAATTCAAGTGATGTAGAGTTGGACACGTTGT
CTCAGGTAGAAGAAGAGTCTCTGTTACGAAATGATCTTCGTCCAGCTAACAAACTTGCTAAAGGAAATAG
ACTATTCATGAGATTTGCTACAAAAGATGACAAAAAGGAACTTGGAGCAGCCAGAAGAAGTCAATACTAC
ATGAAGTATGGAAATCCAAATTATGGAGGCATGAAAGGAATTCTTAGCAATTCATGGAAGCGAAGATATC
ATTCCCGTCGCATTCAGCGGGATGTGATCAAGAAGAGAGCCCTGATTGGGGATGACGTTGGCTTGACGTC
GTATAAACACCGACATTCCGGATTAGTGAATGTTCCTGAGGAACCCATTGAGGAAGAGGAGGAGGAGGAG
GAGGAAGAGGAGGACCAGGACATGGATGCCGATGACAGAGTCGTGGTGGAGTACCATGAGGAGCTCCCAG
GTCTCAAGCAGCCCCGTGAGCGGAGCCTGTCTAGGCGATCCAGTGCGAGCAGCTCAGACTCAGATGAGAT
GGACTATGATCTGGAACTGAAGATGATTTCTACTCCCTCCCCAAAGAAAAGCATGAAAATGACCATGTAT
GCAGATGAAGTAGAGTCCCAACTGAAAAGTATTAGGAATCCCATGAGGGCAGATAGTATATCCACAAGCA
ATATAAAAAACCGAATTGGTAACAAATTGCCTCCTGAGAAATTTGCAGATGTCCGTCATCTCTTAGATGA
AAAGAGGCAGCATTCCTGTCCACGGCCAGCAGTCAGCAGTACTAAACCAGATATTCGCCAGCGGTTAGGA
AAAAGACCGTATTCTCCAGAGAAGGCTTTTAGTAGTAACCAGGTGGTTCGGAGAGAGCCCTCTTCAGATG
TACATAGTAGGCTAGGTGTTCCCAGGCAAGATGTTAAAGGCCTCTACTCTGATACTCGGGAGAGGAAATC
AGGTGGTTTGTGGACTCGCTTAGGATCTACACCCAAGACCAAAGAAAAGAACACGAAGAAAGTGGATCAC
AGGGCATCTGGTGCTGAGGAAGATGACTCGGAGCTTCAGAGAGCATGGGGTGCTCTGATCAAGGAGAAAG
AAGAGTCTCGCCAGAAGAAGAGTCGGCTGGATAGCTTGCCTTCGCTCCAGATTGAAGTCAGCCGGGAAAG
TAGCTCTGGTTCAGAGGCAGAGTCCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_025818
Insert Size 1848 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_025818.3, NP_080094.3
RefSeq Size 6284 bp
RefSeq ORF 1848 bp
Locus ID 66874
UniProt ID Q8BZR9
Gene Summary Associates with NCBP1/CBP80 to form an alternative cap-binding complex (CBC) which plays a key role in mRNA export. NCBP3 serves as adapter protein linking the capped RNAs (m7GpppG-capped RNA) to NCBP1/CBP80. Unlike the conventional CBC with NCBP2 which binds both small nuclear RNA (snRNA) and messenger (mRNA) and is involved in their export from the nucleus, the alternative CBC with NCBP3 does not bind snRNA and associates only with mRNA thereby playing a role in only mRNA export. The alternative CBC is particularly important in cellular stress situations such as virus infections and the NCBP3 activity is critical to inhibit virus growth (PubMed:26382858).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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