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Rpa1 (NM_026653) Mouse Untagged Clone
CAT＃: MC219833
Rpa1 (untagged) - Mouse replication protein A1 (Rpa1), transcript variant 2, (10ug)
CNY 7,980.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 70kDa; 5031405K23Rik; AA589576; AW557552; RF-A; RP-A; Rpa |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC219833 representing NM_026653
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGGACACCTGAGCGAGGGGGCCATCGAGGTCATGATACAGCAGGAAAATACATCCATAAAGCCCA TCCTGCAGGTCATTAACATCCGTCCCATTTCTACAGGGAATAGGTCACCCCGGTACCGACTGCTCATGAG TGATGGGTTGAACACGCTTTCCTCGTTCATGCTGGCCACGCAGCTGAACACCCTGGTCGAGGGAGGGCAG CTGGCCAGCAACTGTGTCTGCCAGGTCCACAAGTTCATCGTCAACACCCTGAAAGACGGCAGGAAAGTAG TTGTTTTGATGGACTTGGAAGTTATGAAATCTGCTGAAGACGTTGGATTAAAGATTGGGAATCCAGTGCC CTATAATGAAGGATATGGGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAAGCAGTCCCTTCCCCA GCCTCTGCAGCGACACCACCAGCCAGCAAGCCCCAGCCACAGAATGGGAGTCTGGGAATGGGTTCCACTG CAGCTAAGGCTTATGGTGCCTCAAAGCCATTTGGAAAACCTGCCGGAACTGGCCTGCTGCAGCCTTCAGG TGGGACACAGTCCAAAGTGGTGCCCATCGCCAGCCTCACCCCGTACCAGTCCAAGTGGACTATTTGTGCC CGTGTCACCAACAAAAGTCAGATCCGCACCTGGAGTAATTCCCGGGGGGAAGGAAAGCTTTTCTCTCTAG AACTTGTGGATGAAAGCGGTGAAATCAGAGCTACTGCTTTCAATGAGCAAGTGGACAAGTTCTTTCCCCT TATTGAAGTGAACAAGGTGTATTACTTCTCAAAGGGCGCCCTGAAGATCGCTAACAAACAGTTCTCCGCT GTTAAAAATGACTATGAGATGACCTTCAATAATGAGACTTCTGTCCTTCCCTGTGAAGATGGCCATCACT TACCCACAGTTCAGTTTGATTTCACAGGGATCGGTGACCTAGAGAGCAAGGCTAAAGACGCACTAGTAGA CATCATTGGAATCTGCAAGAGCTATGAAGATTCGATTAAAATCACAGTGAAGTCGAACAACAGAGAAGTT GCTAAGAGAAATATCTATTTGATGGACATGTCAGGGAAGGTGGTGACCACAACTCTGTGGGGAGAAGATG CTGACAAGTTTGACGGCTCTCGGCAGCCCGTGATGGCCATCAAAGGAGCCCGAGTCTCTGACTTCGGTGG ACGGAGCCTCTCGGTCCTCTCATCCAGCACTGTCATTGTGAATCCTGACATCCCAGAGGCCTATAAGCTT CGTGGCTGGTTTGACTCAGAAGGACAAGCCTTAGATGGTGTTTCCATCTCTGACCACAGGAGTGGAGGGG CCGGAGGGGGCAACACCAACTGGAAAACTTTGCATGAGGCTAAATCTGAGAACCTGGGCCAGGGAGACAA GGCGGACTATTTCAGCACTGTGGCAGCGGTGGTGTTTCTCCGCAAAGAGAACTGTATGTACCAGGCCTGC CCAACCCAGGACTGCAATAAGAAAGTGATTGACCAGCAGAATGGGTTGTACCGCTGTGAGAAGTGTGACC GGGAATTTCCCAATTTCAAATACCGCATGATCTTATCGGCAAATATTGCAGATTTTCAAGAGAACCAGTG GGTGACGTGTTTCCAGGAGTCTGCAGAGGCCATCCTTGGACAGAACACTATGTACCTTGGGGAACTCAAA GAGAAGAACGAGCAGGCGTTTGAAGAGGTTTTCCAGAATGCCAACTTCCGGTCATTTACGTTCAGAATCA GGGTCAAGCTGGAGACCTACAATGATGAATCTCGAATTAAGGCCACTGTGATGGACGTGAAGCCCGTGGA CTTCAGAGACTATGGCAGACGGCTGATCGCGAACATCAGGAAGAACATGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_026653 |
| Insert Size | 1872 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_026653.2, NP_080929.1 |
| RefSeq Size | 3058 bp |
| RefSeq ORF | 1872 bp |
| Locus ID | 68275 |
| UniProt ID | Q8VEE4 |
| Gene Summary | As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. The resulting isoform (2) lacks an alternate segment, compared to isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG209516 | Rpa1 (tGFP-tagged) - Mouse replication protein A1 (Rpa1) |
CNY 5,040.00 |
|
| MR209516 | Rpa1 (Myc-DDK-tagged) - Mouse replication protein A1 (Rpa1), transcript variant 2 |
CNY 4,648.00 |
|
| MR209516L3 | Lenti ORF clone of Rpa1 (Myc-DDK-tagged) - Mouse replication protein A1 (Rpa1), transcript variant 2 |
CNY 6,460.00 |
|
| MR209516L4 | Lenti ORF clone of Rpa1 (mGFP-tagged) - Mouse replication protein A1 (Rpa1), transcript variant 2 |
CNY 7,048.00 |
