Rpa1 (NM_026653) Mouse Untagged Clone

CAT#: MC219833

Rpa1 (untagged) - Mouse replication protein A1 (Rpa1), transcript variant 2, (10ug)



  "NM_026653" in other vectors (4)

CNY 7,980.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 70kDa; 5031405K23Rik; AA589576; AW557552; RF-A; RP-A; Rpa
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC219833 representing NM_026653
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGGGACACCTGAGCGAGGGGGCCATCGAGGTCATGATACAGCAGGAAAATACATCCATAAAGCCCA
TCCTGCAGGTCATTAACATCCGTCCCATTTCTACAGGGAATAGGTCACCCCGGTACCGACTGCTCATGAG
TGATGGGTTGAACACGCTTTCCTCGTTCATGCTGGCCACGCAGCTGAACACCCTGGTCGAGGGAGGGCAG
CTGGCCAGCAACTGTGTCTGCCAGGTCCACAAGTTCATCGTCAACACCCTGAAAGACGGCAGGAAAGTAG
TTGTTTTGATGGACTTGGAAGTTATGAAATCTGCTGAAGACGTTGGATTAAAGATTGGGAATCCAGTGCC
CTATAATGAAGGATATGGGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAAGCAGTCCCTTCCCCA
GCCTCTGCAGCGACACCACCAGCCAGCAAGCCCCAGCCACAGAATGGGAGTCTGGGAATGGGTTCCACTG
CAGCTAAGGCTTATGGTGCCTCAAAGCCATTTGGAAAACCTGCCGGAACTGGCCTGCTGCAGCCTTCAGG
TGGGACACAGTCCAAAGTGGTGCCCATCGCCAGCCTCACCCCGTACCAGTCCAAGTGGACTATTTGTGCC
CGTGTCACCAACAAAAGTCAGATCCGCACCTGGAGTAATTCCCGGGGGGAAGGAAAGCTTTTCTCTCTAG
AACTTGTGGATGAAAGCGGTGAAATCAGAGCTACTGCTTTCAATGAGCAAGTGGACAAGTTCTTTCCCCT
TATTGAAGTGAACAAGGTGTATTACTTCTCAAAGGGCGCCCTGAAGATCGCTAACAAACAGTTCTCCGCT
GTTAAAAATGACTATGAGATGACCTTCAATAATGAGACTTCTGTCCTTCCCTGTGAAGATGGCCATCACT
TACCCACAGTTCAGTTTGATTTCACAGGGATCGGTGACCTAGAGAGCAAGGCTAAAGACGCACTAGTAGA
CATCATTGGAATCTGCAAGAGCTATGAAGATTCGATTAAAATCACAGTGAAGTCGAACAACAGAGAAGTT
GCTAAGAGAAATATCTATTTGATGGACATGTCAGGGAAGGTGGTGACCACAACTCTGTGGGGAGAAGATG
CTGACAAGTTTGACGGCTCTCGGCAGCCCGTGATGGCCATCAAAGGAGCCCGAGTCTCTGACTTCGGTGG
ACGGAGCCTCTCGGTCCTCTCATCCAGCACTGTCATTGTGAATCCTGACATCCCAGAGGCCTATAAGCTT
CGTGGCTGGTTTGACTCAGAAGGACAAGCCTTAGATGGTGTTTCCATCTCTGACCACAGGAGTGGAGGGG
CCGGAGGGGGCAACACCAACTGGAAAACTTTGCATGAGGCTAAATCTGAGAACCTGGGCCAGGGAGACAA
GGCGGACTATTTCAGCACTGTGGCAGCGGTGGTGTTTCTCCGCAAAGAGAACTGTATGTACCAGGCCTGC
CCAACCCAGGACTGCAATAAGAAAGTGATTGACCAGCAGAATGGGTTGTACCGCTGTGAGAAGTGTGACC
GGGAATTTCCCAATTTCAAATACCGCATGATCTTATCGGCAAATATTGCAGATTTTCAAGAGAACCAGTG
GGTGACGTGTTTCCAGGAGTCTGCAGAGGCCATCCTTGGACAGAACACTATGTACCTTGGGGAACTCAAA
GAGAAGAACGAGCAGGCGTTTGAAGAGGTTTTCCAGAATGCCAACTTCCGGTCATTTACGTTCAGAATCA
GGGTCAAGCTGGAGACCTACAATGATGAATCTCGAATTAAGGCCACTGTGATGGACGTGAAGCCCGTGGA
CTTCAGAGACTATGGCAGACGGCTGATCGCGAACATCAGGAAGAACATGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_026653
Insert Size 1872 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_026653.2, NP_080929.1
RefSeq Size 3058 bp
RefSeq ORF 1872 bp
Locus ID 68275
UniProt ID Q8VEE4
Gene Summary As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. The resulting isoform (2) lacks an alternate segment, compared to isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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