Ctcfl (NM_001081387) Mouse Untagged Clone

CAT#: MC219960

Ctcfl (untagged) - Mouse CCCTC-binding factor (zinc finger protein)-like (Ctcfl), (10ug)



  "NM_001081387" in other vectors (4)

CNY 8,080.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Boris; OTTMUSG00000016680
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC219960 representing NM_001081387
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCCGCTGAGGTCCCTGTCCCTTCTGGGTACTTCACCCAGATCAAAGAGCAGAAGTTGAAGCCTG
GAGACCTAGAGGAGGAGAAAGAGGAGGACGGGGTACAAAGAGTGGAAGCCCAGGAGGGAGTTGTCAAGGA
GGTGGAGGCCGAGAACAGTTGCCTGCTTCTGGAGGCCAGGGCCCCGGTGGAGAGCGACAGGCGGATCCTG
ACCCTGCAAACGGTGCACCTGGAGTCCCAGGATGTGCACCTACAGGGGCTGGGATGGCTGAGCGTGCCAC
ACTCTGAGGAGCTTTCAGGGACGGTACCAGAGGCGGAAGGCATACTGCAGTTGCCATCCGTGCTGTGGCT
CGACCCAGAGCCCCAGCTCAGCCTTCAGCATTGCGTGACGGTCAGCATCCCGGAAGAGCTGTACCCACCA
GAGGAGCTGCAGCGGATACATTTTCACCTGCTGAGAGAGAATGTGCTAATGGCCGAGGAGAACCCAGAGT
TAACACCAGACTTGGACGAAAGCACAGCCCTGAAAAAGCCCGAAGAAGATGAAAAGGACCAGCTCCCGCC
CCAGGGAGAGACAGACAAGAGAGAAGAGAGGTTGCTCCTTCTGGAAATGAAACCAAAAGAGGGAAAAGAC
GACGAAATTGTCCTGACCATTTCCCATCTAAGCCTCGAAGAACAGCAAGATCCACCAGCGGCCAATCAGA
CAAGTGTGCCGGGAGCCAAAGCCGCAAAACCAAAACGGCGGAGGCAGACCAAGGGAAAGCCTCAGAGCTT
TCAGTGTGACACCTGCCCGTTCACTTCCTCCAAGCTCTCAACTTTCAATCGTCACATCAAAATTCACAGC
AATGAGAGGCCACACCTGTGTCACCTGTGCCTGAAGGCCTTCCGGACTGTCACTCTTCTTAGGAACCATG
TGAACACCCACACAGGAACCAGGCCCCACAAGTGCAGGGACTGCGACATGGCGTTTGTCACCAGTGGAGA
ACTCGTCCGGCACAGGCGTTACAAACACACTTATGAGAAGCCCTTCAAGTGCTCCCTGTGCAAGTACGCC
AGCGTCGAGGCAAGCAAGATGAAGCGTCACATCCGCTCACACACGGGTGAGCGTCCCTTCCAGTGTTGCC
AGTGTGCTTACGCCAGCAGGGACTCCTACAAGCTGAAGCGCCACATGAGGACACACTCAGGTGAGAAGCC
GTATGAATGTCCCACCTGTCACGTCCGGTTCACCCAGAGCGGGACCATGAAAATCCATATAGCACAGAAG
CACGGAGAGAATGTGCCCAAATACGAGTGTCCCCACTGTGCCACCATCATCGCGAGGAAGAGCGACCTGC
GTGTCCATCTGCGTAACCTGCACAGCCAGAGCCCGGAGGAGATGAAGTGCCGATACTGTCCCGCTGGCTT
CCATGAGCGCTATGCCCTCATTCAGCACCAGAGGACCCACAAGAACGAGAAGAAGTTCAAGTGCAAGCAG
TGCGATTACGCGTGCAAGCAGGAGCGATGCTTGAAGGCGCACATGCGCATGCACACAGGAGAGAAGCCCT
TCTCCTGCCTGGCCTGCAACAAGCACTTCCGACAGAAGCAGCTACTGACCGTGCACCTGAGGAAGTACCA
TGACCCGAACTTCGTCCCCAATCTGCACCTGTGCCTCAAGTGTGATAAACGTTTCTCCCGCTGGAGTAAC
CTGCAGAGACACAGAAAGAAGTGTGACCCGGAGCATGAGACGTTAGCCCCCAACAAGGACAGGAGACCAG
TGACAAGGACACAGGCCTCGGAGGGAGAAGCAGGACACAAGGAAGGGGAGCCTCAGTGCCCTGGGGAGCA
GGCTCTGGGCCACCAAGGAGAAGCAGCGGGGAGCCAGAGCCCAGACCACGGCCTTACCTGCGAGATAATC
TTTAACATGATGGATAAGTGA


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-RsrII     
ACCN NM_001081387
Insert Size 1911 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001081387.2, NP_001074856.1
RefSeq Size 3217 bp
RefSeq ORF 1911 bp
Locus ID 664799
UniProt ID A2APF3
Gene Summary Testis-specific DNA binding protein responsible for insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. Plays a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2. This probably leads to recruit de novo DNA methyltransferases at these sites. Seems to act as tumor suppressor. In association with DNMT1 and DNMT3B, involved in activation of BAG1 gene expression by binding to its promoter. Required for dimethylation of H3 lysine 4 (H3K4me2) of MYC and BRCA1 promoters.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) uses an alternate in-frame 3' splice junction compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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