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Rpa1 (NM_001164223) Mouse Untagged Clone
CAT＃: MC220005
Rpa1 (untagged) - Mouse replication protein A1 (Rpa1), transcript variant 1, (10ug)
CNY 8,270.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 70kDa; 5031405K23Rik; AA589576; AW557552; RF-A; RP-A; Rpa |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC220005 representing NM_001164223
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGGACACCTGAGCGAGGGGGCCATCGAGGTCATGATACAGCAGGAAAATACATCCATAAAGCCCA TCCTGCAGGTCATTAACATCCGTCCCATTTCTACAGGGAATAGGTCACCCCGGTACCGACTGCTCATGAG TGATGGGTTGAACACGCTTTCCTCGTTCATGCTGGCCACGCAGCTGAACACCCTGGTCGAGGGAGGGCAG CTGGCCAGCAACTGTGTCTGCCAGGTCCACAAGTTCATCGTCAACACCCTGAAAGACGGCAGAATGCAAC CAAAACCTCATGGGAATCCGTATGGTGAACAGTCAGTGCTGAGGAAGTTAAGCCAGAAAGTAGTTGTTTT GATGGACTTGGAAGTTATGAAATCTGCTGAAGACGTTGGATTAAAGATTGGGAATCCAGTGCCCTATAAT GAAGGATATGGGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAAGCAGTCCCTTCCCCAGCCTCTG CAGCGACACCACCAGCCAGCAAGCCCCAGCCACAGAATGGGAGTCTGGGAATGGGTTCCACTGCAGCTAA GGCTTATGGTGCCTCAAAGCCATTTGGAAAACCTGCCGGAACTGGCCTGCTGCAGCCTTCAGGTGGGACA CAGTCCAAAGTGGTGCCCATCGCCAGCCTCACCCCGTACCAGTCCAAGTGGACTATTTGTGCCCGTGTCA CCAACAAAAGTCAGATCCGCACCTGGAGTAATTCCCGGGGGGAAGGAAAGCTTTTCTCTCTAGAACTTGT GGATGAAAGCGGTGAAATCAGAGCTACTGCTTTCAATGAGCAAGTGGACAAGTTCTTTCCCCTTATTGAA GTGAACAAGGTGTATTACTTCTCAAAGGGCGCCCTGAAGATCGCTAACAAACAGTTCTCCGCTGTTAAAA ATGACTATGAGATGACCTTCAATAATGAGACTTCTGTCCTTCCCTGTGAAGATGGCCATCACTTACCCAC AGTTCAGTTTGATTTCACAGGGATCGGTGACCTAGAGAGCAAGGCTAAAGACGCACTAGTAGACATCATT GGAATCTGCAAGAGCTATGAAGATTCGATTAAAATCACAGTGAAGTCGAACAACAGAGAAGTTGCTAAGA GAAATATCTATTTGATGGACATGTCAGGGAAGGTGGTGACCACAACTCTGTGGGGAGAAGATGCTGACAA GTTTGACGGCTCTCGGCAGCCCGTGATGGCCATCAAAGGAGCCCGAGTCTCTGACTTCGGTGGACGGAGC CTCTCGGTCCTCTCATCCAGCACTGTCATTGTGAATCCTGACATCCCAGAGGCCTATAAGCTTCGTGGCT GGTTTGACTCAGAAGGACAAGCCTTAGATGGTGTTTCCATCTCTGACCACAGGAGTGGAGGGGCCGGAGG GGGCAACACCAACTGGAAAACTTTGCATGAGGCTAAATCTGAGAACCTGGGCCAGGGAGACAAGGCGGAC TATTTCAGCACTGTGGCAGCGGTGGTGTTTCTCCGCAAAGAGAACTGTATGTACCAGGCCTGCCCAACCC AGGACTGCAATAAGAAAGTGATTGACCAGCAGAATGGGTTGTACCGCTGTGAGAAGTGTGACCGGGAATT TCCCAATTTCAAATACCGCATGATCTTATCGGCAAATATTGCAGATTTTCAAGAGAACCAGTGGGTGACG TGTTTCCAGGAGTCTGCAGAGGCCATCCTTGGACAGAACACTATGTACCTTGGGGAACTCAAAGAGAAGA ACGAGCAGGCGTTTGAAGAGGTTTTCCAGAATGCCAACTTCCGGTCATTTACGTTCAGAATCAGGGTCAA GCTGGAGACCTACAATGATGAATCTCGAATTAAGGCCACTGTGATGGACGTGAAGCCCGTGGACTTCAGA GACTATGGCAGACGGCTGATCGCGAACATCAGGAAGAACATGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001164223 |
| Insert Size | 1935 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001164223.1, NP_001157695.1 |
| RefSeq Size | 3121 bp |
| RefSeq ORF | 1935 bp |
| Locus ID | 68275 |
| UniProt ID | Q8VEE4 |
| Gene Summary | As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG222928 | Rpa1 (tGFP-tagged) - Mouse replication protein A1 (Rpa1) transcript variant 1, (10ug) |
CNY 7,130.00 |
|
| MR222928 | Rpa1 (Myc-DDK-tagged) - Mouse replication protein A1 (Rpa1), transcript variant 1 |
CNY 7,200.00 |
|
| MR222928L3 | Lenti ORF clone of Rpa1 (Myc-DDK-tagged) - Mouse replication protein A1 (Rpa1), transcript variant 1 |
CNY 8,360.00 |
|
| MR222928L4 | Lenti ORF clone of Rpa1 (mGFP-tagged) - Mouse replication protein A1 (Rpa1), transcript variant 1 |
CNY 8,360.00 |
