United States
Germany
Japan
United Kingdom
China
Pot1a (NM_133931) Mouse Untagged Clone
CAT＃: MC220025
Pot1a (untagged) - Mouse protection of telomeres 1A (Pot1a), (10ug)
CNY 7,160.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 1500031H18Rik; AI851169; Pot1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC220025 representing NM_133931
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTTTGGTTTCAACAGCTCCCTATACATATACACCCCTGAATCTACTCAAGGAAGGTACAATTGCCA ATGTCTATGGTGTTGTGAAATTCTTTAAGCCTCCGTATGTTAGCAAAGGAACTGATTATTGCTCAGTTGT AACAATTGTGGACCAGACAAATGTGAAGTTAACCTGTATGCTCTTTAGTGGAAACTATGAAGCCCTCCCC ATCATTTATAAAGTTGGAGACATTGTTCGCTTCCACAGGCTGAAGATCCAAGTGTATAAAAATGAGCTTC AGGGAATCAACTGCTCTGGCTTCGCCTCTTTGACATTTGAAGGGACTGTAGGGATGCCTGTCACAGCACG CACTTCCAGCAAAGTTTTTAGCTTCACTCCTCAGGACCAAAAAATGGTAGAAGCTTTGCGGGTTTGGGCA TCCAAGCACATCTCGGCGTCTTCAACTTTAGTGCAGCTGTGCGATGCGCAGCCCATGCAGTATTACGACC TGACCTGTCAGCTCCTGGGTAAAGCACAAGTGGATTCCACAGCATTTCTTCTAAAGGTATGGGATGGAAC CCAGACTGTGCTTCCCTCCTGGAGAGTCTCCACACAAGATCTAACTTTTGAAGGTGACTTGAGTCACATC GAACGGCTACAGAGTCTGGTGGTAGACATTCTAGTCTACGATAACCACGTTCAAGTGGCGAGATCCATCG AGGTTGGATGCTTTCTAAGACTCTATAGCCTCCATACAAAACTTCAGCCTGGAAATTCAGAAACCAGCAG TTCTGAATCATTAAGGCTGGAGTTCCATCTCCACGGAGGGACCAGTTATGGTCGGGGCATTAGGGTTTTG CCAGATACAAGCCCTTGTGTGGATCAGCTGAAAAAAGCTTTAGAAGGTGCAAATTTGCCAGTCACTGAGA CCTCAACTGGTATCTGTCAATCAGAAAATGGGGACAGCTCTGCACTATCAAACTCAGGATCAGGTGCAGT ATCACCTTATGAAGAAGAACGATGTCAACAAGTCTCTGCTACAATACTTACCAATCACCAACATTTAGAG AAAACACCACTGTGTGCCATCTTGACGCAAAAAGCTCCTCAACAATATCGAGTCCGCGCCAAACTGAGAT CCTATTTGCCCAGGAGACTTTCCCAGTCTGTTAAACTTCTCTGTCCTAAATGTCATTCAGTACAAGAAGT TCCACATGGGGACAGTTTGGATAAAATTTTGCAAGATGCTGCAACTGAAGCTCCAGACATCAAGCTGAAA GCGACATCATTGTATTATTCAAAAGTCTGGACCACTGAAGACCAGGGCGGACGGCAGGTCGCTGTTCATT TTGTGAAAAATAATGGCATTCTCCCTGCTTCAAGTGAATGTCTAATTTTGATAGAAGGAGGAAGGCTCTG CGAAGTCTCTAAACTGTCCAGCAAGTTCCACAGTGTGATGCCTGTGCGGTCCGGCCCTGAGAGCCTGGAG CTCCTTACCCTTTCAGCCCCGTTCCTCATACAGGGAAAGGTGCATCACTATGGATGTAAACAGTGCTCAA GCTTGAAACCTATACAAAATCTAAACTCCCGTTTTCATAAAGGACCGTGGACTCCTTCCTCTGTGGCAGA AGCCCTGGGCGTTGTGCCGCTGCAGTATGTGTTTGTTATGGTTTTCACTCTGGATGACGGGACAGGGGTG TTGGAAGCTTACCTCAAGGATTCTGAACACTTTTTCAAGATCCCAGCATCTGAAGTCCTCACTGATGACG ACCTTCAGAGAAGTCTAGAAACGATCATGGATATGATATGTCCCCCAGGAATAAAAGTTGATGCATACCC ATGGCTAGAGTGCCTCCTCAAGTCCTACAATGTCACCATCGGAACAGAGCGACGAATTTGCTACCAGATT TTTGATACTACAGTTGCAGAAAATGTTGTCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_133931 |
| Insert Size | 1923 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_133931.4, NP_598692.1 |
| RefSeq Size | 3153 bp |
| RefSeq ORF | 1923 bp |
| Locus ID | 101185 |
| UniProt ID | Q91WC1 |
| Gene Summary | Component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. Is a component of the double-stranded telomeric DNA-binding TRF1 complex which is involved in the regulation of telomere length by cis-inhibition of telomerase. Also acts as a single-stranded telomeric DNA-binding protein and thus may act as a downstream effector of the TRF1 complex and may transduce information about telomere maintenance and/or length to the telomere terminus. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Binds to two or more telomeric single-stranded 5'-TTAGGG-3' repeats (G-strand) and with high specificity to a minimal telomeric single-stranded 5'-TAGGGTTAG-3' sequence. Binds telomeric single-stranded sequences internally or at proximity of a 3'-end. Its activity is TERT dependent but it does not increase TERT activity (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The telomere binding protein Pot1 maintains haematopoietic stem cell activity with age
,Hosokawa, K;MacArthur, BD;Ikushima, YM;Toyama, H;Masuhiro, Y;Hanazawa, S;Suda, T;Arai, F;,
Nat Commun
,PubMed ID 28986560
[POT1A]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG209691 | Pot1a (tGFP-tagged) - Mouse protection of telomeres 1A (Pot1a) |
CNY 8,744.00 |
|
| MR209691 | Pot1a (Myc-DDK-tagged) - Mouse protection of telomeres 1A (Pot1a) |
CNY 7,144.00 |
|
| MR209691L3 | Lenti ORF clone of Pot1a (Myc-DDK-tagged) - Mouse protection of telomeres 1A (Pot1a) |
CNY 8,270.00 |
|
| MR209691L4 | Lenti ORF clone of Pot1a (mGFP-tagged) - Mouse protection of telomeres 1A (Pot1a) |
CNY 8,270.00 |
