Foxk2 (NM_001080932) Mouse Untagged Clone

CAT#: MC220094

Foxk2 (untagged) - Mouse forkhead box K2 (Foxk2), (10ug)



  "NM_001080932" in other vectors (4)

CNY 8,360.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 1110054H05Rik; 5730434B08Rik; 6230415M23Rik; ILF; Ilf1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC220094 representing NM_001080932
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCCGCGGCGGCGCTCTCGGGCGCGGGCGCCCCTCCCGCGGGCGGCGGGGCCGGGGGCGGCGGAT
CCCCGCCGGGCGGCTGGGCCGTGGCGCGGCTCGAAGGCCGCGAGTTCGAGTACCTCATGAAGAAGCGGTC
GGTGACCATCGGCCGCAACTCATCGCAGGGCTCGGTAGATGTGAGCATGGGCCACTCGAGCTTCATCTCG
CGGCGCCACCTGGAGATCTTCACGCCCCCGGGCGGCGGCCATAGCGCGGCCGCCCCGGAACCCGCGCAGC
CCCGGCCGGACGCGGGCGGCGACTTCTACCTGCGCTGTCTCGGCAAGAACGGCGTGTTCGTGGACGGGGT
GTTCCAGCGGCGGGGGGCGCCGCCGCTGCAGCTGCCGCGCGTGTGCACTTTCAGGTTTCCGAGCACAAAT
ATCAAGATAACATTCACAGCCCTATCCAGCGAGAAGAGAGAGAAGCAGGAAGCACCTGAGTCGCCAGTGA
AGCCTGTGCAGCCACACATCTCACCCCTGACCATCAACATTCCAGACACGATGGCCCACCTCATCAGCCC
CCTTCCTTCCCCCACGGGAACTATCAGTGCTGCCAACTCCTGCCCATCCAGTCCTCGCGGAGCAGGGTCT
TCAGGGTACAAAGTGGGCCGTGTGATGCCATCGGACCTCAGTTTAATGGCTGACAACTCCCAGCCAGAAA
ATGAAAAGGAAGCGTCAGGTGGAGACAGCCCAAAGGATGACTCAAAGCCACCTTACTCCTATGCACAGTT
GATAGTACAAGCAATTACAATGGCTCCTGATAAACAACTTACCCTAAATGGGATTTATACACACATCACT
AAAAACTATCCATACTACAGGACTGCGGACAAGGGCTGGCAGAATTCAATTCGCCACAATCTCTCTCTGA
ACCGTTATTTCATCAAAGTGCCACGTTCCCAGGAAGAACCAGGCAAAGGCTCCTTCTGGAGGATAGACCC
TGCCTCCGAGAGCAAGCTGGTAGAGCAGGCTTTTAGGAAAAGACGGCCTAGGGGCGTGCCTTGCTTTAGA
ACCCCCCTGGGACCTCTCTCCTCTAGGAGTGCCCCAGCTTCTCCCAACCATGCCGGAGTGCTGTCTGCTC
ACTCCAGTGGCGCCCAGACCCCCGAGAGCCTGTCGAGGGAGGGCTCCCCAGCCCCCTTGGAGCCTGAGCC
TGGCGCCTCACAGCCCAAGCTTGCCGTCATCCAGGAGGCCAGGTTTGCCCAGAGTGCACCAGGGTCACCT
CTCTCTAGTCAGCCCGTCTTAATTACTGTCCAGCGGCAGCTACCCCCGGCCATTAAGCCTGTCACCTACA
CTGTTGCAACCCCAGTGACCACACCCACCTCTCAGCCACCTGTTGTGCAGACGGTCCATGTTGTTCATCA
GATACCAGCAGTGTCAGTCACCAGTGTGGCTGGACTGGCCCCAGCAAACACATACACAGTTGCTGGACAA
GCTGTGGTCACTCAGGCTGCGGTGCTGGCTCCTCCTAACCCTGAACCACAAGAGAATGGCGACCACAGAG
AAGTCAGAGTGAAAGTAGAACCTGTCCCTGCAATTAGCCCAGCTACACTAGGCGCTGCTAGCCGAATCAT
CCAGACATCACAGGGCACCCCTGTTCAGACAGTGACCATAGTGCAGCAGGCGCCTTTAGGTCAACACCAG
CTTCCAATAAAAACTGTAACGCAAAACGGAGCTCATGTGGTACCCATGCCTACAGCAGTGCACAGCCAGG
TGAACAATGCAGCAGCAAGTCCTCTCCATATGTTGGCCACCCATGCATCAGCATCGGCATCCCTGCCCAC
AAAGCGCCAGAACGGTGACCAGGCAGAGCAGCCAGAGCTCAAGCGAGTTAAAGCAGAAGATGGCGAGAGC
ATTGTCATTGCCCTGAGTGTGGATGCACCACCAGCAGCTGTGAGAGAAAAGGCAATCCAGAACTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001080932
Insert Size 1956 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001080932.2, NP_001074401.2
RefSeq Size 5032 bp
RefSeq ORF 1956 bp
Locus ID 68837
UniProt ID Q3UCQ1
Gene Summary Transcriptional regulator involved in different processes such as glucose metabolism, aerobic glycolysis and autophagy (PubMed:25402684, PubMed:29861159, PubMed:30700909). Recognizes and binds the forkhead DNA sequence motif (5'-GTAAACA-3') and can both act as a transcription activator or repressor, depending on the context (PubMed:25402684, PubMed:29861159, PubMed:30700909). Together with FOXK1, acts as a key regulator of metabolic reprogramming towards aerobic glycolysis, a process in which glucose is converted to lactate in the presence of oxygen (PubMed:30700909). Acts by promoting expression of enzymes for glycolysis (such as hexokinase-2 (HK2), phosphofructokinase, pyruvate kinase (PKLR) and lactate dehydrogenase), while suppressing further oxidation of pyruvate in the mitochondria by up-regulating pyruvate dehydrogenase kinases PDK1 and PDK4 (PubMed:30700909). Probably plays a role in gluconeogenesis during overnight fasting, when lactate from white adipose tissue and muscle is the main substrate (PubMed:30700909). Together with FOXK1, acts as a negative regulator of autophagy in skeletal muscle: in response to starvation, enters the nucleus, binds the promoters of autophagy genes and represses their expression, preventing proteolysis of skeletal muscle proteins (PubMed:25402684). In addition to the 5'-GTAAACA-3' DNA motif, also binds the 5'-TGANTCA-3' palindromic DNA motif, and co-associates with JUN/AP-1 to activate transcription (By similarity). Also able to bind to a minimal DNA heteroduplex containing a G/T-mismatch with 5'-TRT[G/T]NB-3' sequence (By similarity). Binds to NFAT-like motifs (purine-rich) in the IL2 promoter (By similarity). Positively regulates WNT/beta-catenin signaling by translocating DVL proteins into the nucleus (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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