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Katnb1 (NM_028805) Mouse Untagged Clone
CAT＃: MC220189
Katnb1 (untagged) - Mouse katanin p80 (WD40-containing) subunit B 1 (Katnb1), (10ug)
CNY 8,460.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 2410003J24Rik; KAT |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC220189 representing NM_028805
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACCCCTGTGGTCACCAAGACAGCCTGGAAGTTGCAAGAGATCGTCGCCCATGCCAGCAATGTGT CCTCCTTGGTGCTGGGCAAGGCCTCAGGGCGGCTGCTGGCCACTGGTGGGGATGACTGCCGAGTCAACCT GTGGTCCATAAACAAGCCTAACTGCATCATGAGCCTGACAGGTCACACGTCCCCTGTGGAGAGTGTCCGT CTCAACACTCCTGAGGAGCTCATCGTAGCTGGTTCCCAGTCGGGCTCCATCCGCGTCTGGGACCTGGAAG CTGCTAAAATTCTTCGAACACTCATGGGCCACAAAGCCAACATCTGCAGCCTGGATTTCCACCCCTATGG TGAATTTGTGGCCTCAGGCTCCCAGGACACAAACATTAAGCTCTGGGACATCAGGAGGAAAGGCTGTGTC TTCCGATATAGGGGGCACAGCCAGGCTGTGCGGTGTCTCCGGTTCAGCCCTGATGGGAAGTGGCTGGCGT CGGCAGCAGATGACCACACAGTGAAGCTTTGGGATCTGACGGCTGGCAAGATGATGTCCGAGTTCCCGGG TCATACAGGGCCTGTCAATGTGGTGGAGTTTCACCCCAATGAATATCTCCTAGCTTCTGGCAGCTCCGAC AGGACAATCCGCTTCTGGGACCTGGAGAAGTTCCAGGTGGTGAGCTGCATTGAAGGGGAGCCCGGGCCCG TCAGGAGTGTCCTCTTCAACCCCGACGGCTGCTGCTTGTACAGCGGCTGCCAGGACTCGCTGCGTGTCTA CGGCTGGGAACCTGAGCGCTGCTTTGATGTGGTCCTTGTCAACTGGGGCAAGGTGGCTGACCTGGCCATC TGCAATGACCAGCTGATAGGTGTGGCTTTCTCCCAGAGTAATGTCTCCTCTTATGTGGTGGACCTGACGA GAGTCACCAGGACGGGAACTGTGACCCAGGACCCTGTGCAGGCTAACCAGCCTCTGACACAACAGACCCC TAACCCTGGTGTCTCCCTCCGCCGCATCTATGAGCGGCCCAGCACGACATGCAGCAAGCCTCAGAGGGTA AAGCACAACTCAGAGAGCGAGCGGCGCAGCCCCAGCAGCGAAGATGACAGGGACGAGCGAGAGTCCCGGG CGGAGATCCAGAACGCGGAGGACTACAATGAGATTTTCCAGCCCAAGAACAGCATCAGTCGGACACCACC CCGGAGAAGTGAGCCCTTCCCAGCACCCCCAGAAGATGATGCAGCCACAGTGAAGGAGGTCTCAAAACCC AGCCCAGCCATGGATGTGCAGTTGCCGCAGTTGCCGGTGCCAAATCTCGAGGTCCCAGCACGACCGTCAG TCATGACTTCCACTCCTGCACCCAAGGGTGAACCTGACATCATCCCTGCCACACGGAATGAGCCCATTGG CTTAAAGGCCTCGGACTTCTTGCCCGCCGTGAAGGTCCCCCAGCAGGCAGAGCTGGTGGACGAGGATGCC ATGTCGCAGATACGCAAAGGCCACGACACCATGTTTGTGGTGCTCACCAGCCGCCACAAGAACCTGGACA CTGTCCGAGCTGTATGGACTACAGGAGATATCAAGACATCAGTGGACTCTGCAGTGGCCATCAATGACCT GTCTGTGGTAGTGGACCTCCTGAACATTGTCAATCAGAAAGCCTCCCTGTGGAAGCTGGACCTGTGCACC ACAGTGCTGCCCCAGATCGAGAAACTGCTGCAGAGCAAGTATGAGAGCTATGTCCAGACCGGGTGCACCT CCCTGAAGCTGATCCTGCAGCGGTTCCTACCCCTCATCACTGACATTTTGGCAGCCCCGCCCTCTGTGGG TGTGGACATCAGCCGAGAGGAGAGGTTGCACAAGTGTCGACTCTGCTTCAAACAACTCAAGAGCATCAGT GGCCTCGTCAAGAGCAAGTCGGGTTTGAGTGGCCGCCATGGCAGTGCCTTCCGTGAGCTGCACCTACTCA TGGCCAGTTTGGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_028805 |
| Insert Size | 1977 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_028805.2, NP_083081.2 |
| RefSeq Size | 3425 bp |
| RefSeq ORF | 1977 bp |
| Locus ID | 74187 |
| UniProt ID | Q8BG40 |
| Gene Summary | Participates in a complex which severs microtubules in an ATP-dependent manner. May act to target the enzymatic subunit of this complex to sites of action such as the centrosome. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome. The function in regulating microtubule dynamics at spindle poles seems to depend on the association of the katanin KATNA1:KATNB1 complex with ASPM which recruits it to microtubules. Reversely KATNA1:KATNB1 can enhance ASPM blocking activity on microtubule minus-end growth. Microtubule release within the cell body of neurons may be required for their transport into neuronal processes by microtubule-dependent motor proteins. This transport is required for axonal growth.[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG223741 | Katnb1 (tGFP-tagged) - Mouse katanin p80 (WD40-containing) subunit B 1 (Katnb1), (10ug) |
CNY 5,040.00 |
|
| MR223741 | Katnb1 (Myc-DDK-tagged) - Mouse katanin p80 (WD40-containing) subunit B 1 (Katnb1) |
CNY 4,896.00 |
|
| MR223741L3 | Lenti ORF clone of Katnb1 (Myc-DDK-tagged) - Mouse katanin p80 (WD40-containing) subunit B 1 (Katnb1) |
CNY 6,460.00 |
|
| MR223741L4 | Lenti ORF clone of Katnb1 (mGFP-tagged) - Mouse katanin p80 (WD40-containing) subunit B 1 (Katnb1) |
CNY 6,460.00 |
