Alox15 (NM_009660) Mouse Untagged Clone

CAT#: MC220233

Alox15 (untagged) - Mouse arachidonate 15-lipoxygenase (Alox15), (10ug)



  "NM_009660" in other vectors (4)

CNY 7,416.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 12-LO; 12/15-LO; 15-LOX; Alox12l; L-12LO
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC220233 representing NM_009660
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGTGTCTACCGCATCCGCGTCTCCACCGGGGACTCCGTGTACGCGGGCTCCAACAACGAGGTCTACC
TGTGGTTGATCGGACAGCATGGGGAGGCATCTCTCGGGAAGCTGTTCCGACCCTGTCGGAACTCGGAGGC
AGAATTCAAGGTGGATGTGTCAGAATACCTTGGGCCACTGCTGTTTGTGAGAGTGCAGAAATGGCATTAT
CTCAAAGAGGACGCCTGGTTCTGCAACTGGATTTCTGTGAAGGGCCCCGGAGACCAGGGATCGGAGTACA
CGTTCCCCTGTTACCGATGGGTTCAGGGCACCAGCATCCTGAACCTCCCTGAGGGCACTGGCTGCACCGT
GGTTGAAGACTCTCAAGGCCTGTTCAGGAACCACAGGGAGGAGGAACTGGAAGAAAGGAGGAGTCTGTAC
AGGTGGGGCAACTGGAAGGATGGCACAATCCTGAACGTGGCGGCGACCAGTATCTCTGACCTCCCTGTAG
ACCAGCGATTTCGAGAGGACAAAAGACTTGAATTTGAAGCTTCACAGGTTCTGGGGACAATGGACACCGT
TATTAACTTCCCTAAAAACACTGTGACCTGCTGGAAAAGCCTAGATGACTTCAACTATGTTTTCAAGAGT
GGCCACACCAAGATGGCTGAGCGGGTTCGAAACTCCTGGAAAGAGGATGCTTTCTTTGGGTACCAGTTTC
TCAATGGTGCTAACCCCATGGTGCTGAAGCGGTCTACTTGTCTCCCTGCCCGCCTGGTATTCCCTCCGGG
GATGGAGAAGCTACAGGCCCAGCTGGATGAGGAGCTCAAGAAAGGCACTCTGTTTGAAGCGGATTTCTTC
CTTCTGGATGGGATCAAGGCCAATGTCATCCTTTGTAGTCAGCAGTACCTGGCTGCCCCCCTCGTCATGC
TGAAGCTGCAGCCCGATGGGCAACTCTTGCCTATAGCCATCCAGCTCGAACTGCCCAAAACTGGGTCTAC
CCCACCGCCGATTTTCACGCCCTTGGATCCCCCAATGGACTGGCTCCTGGCCAAATGCTGGGTCCGAAGC
TCAGACTTACAGCTTCATGAGCTACAGGCTCATCTTCTGAGGGGACACTTGGTGGCTGAGGTCTTTGCTG
TGGCCACCATGAGGTGCCTGCCTTCCGTGCACCCTGTTTTTAAGCTTCTAGTTCCTCACCTACTCTACAC
CATGGAAATCAATGTCCGGGCCAGGAGCGACCTGATCTCAGAGAGAGGCTTTTTTGACAAGGTGATGAGC
ACAGGTGGAGGAGGCCACCTGGATCTTCTCAAGCAAGCTGGAGCCTTCCTGACCTACAGCTCATTGTGTC
CCCCTGATGACTTGGCTGAGCGAGGACTCCTGGATATTGACACTTGCTTCTATGCTAAAGATGCCCTGCA
GCTCTGGCAAGTCATGAATCGGTACGTGGTGGGAATGTTCGATCTCTACTACAAGACCGACCAAGCTGTT
CAGGATGACTATGAACTGCAGAGCTGGTGTCAAGAGATCACTGAGATTGGGTTGCAAGGTGCCCAGGACA
GAGGCTTCCCCACCTCCCTCCAGTCCCGGGCTCAGGCTTGCCACTTCATCACCATGTGCATCTTCACATG
CACTGCGCAGCACTCTTCCATCCATCTTGGCCAGCTGGATTGGTTCTACTGGGTTCCTAATGCACCCTGC
ACCATGCGGCTGCCACCACCTAAAACCAAGGACGCGACGATGGAGAAGCTGATGGCGACGCTGCCCAATC
CTAATCAGTCTACTCTCCAGATAAATGTCGTTTGGCTCCTGGGCAGACGCCAGGCTGTTATGGTGCCCCT
GGGCCAGCATTCAGAGGAACACTTCCCAAACCCTGAGGCCAAAGCTGTGCTGAAGAAGTTCAGAGAGGAG
CTGGCTGCCTTGGATAAGGAAATTGAGATTCGTAACAAGAGCTTGGACATACCTTATGAGTACCTGCGGC
CCAGCCTGGTAGAAAACAGCGTGGCCATATGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_009660
Insert Size 1992 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_009660.3, NP_033790.3
RefSeq Size 2414 bp
RefSeq ORF 1992 bp
Locus ID 11687
UniProt ID P39654
Gene Summary Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Converts arachidonic acid into 12-hydroperoxyeicosatetraenoic acid/12-HPETE and 15-hydroperoxyeicosatetraenoic acid/15-HPETE. Also converts linoleic acid to 13-hydroperoxyoctadecadienoic acid. May also act on (12S)-hydroperoxyeicosatetraenoic acid/(12S)-HPETE to produce hepoxilin A3. Probably plays an important role in the immune and inflammatory responses. Through the oxygenation of membrane-bound phosphatidylethanolamine in macrophages may favor clearance of apoptotic cells during inflammation by resident macrophages and prevent an autoimmune response associated with the clearance of apoptotic cells by inflammatory monocytes. In parallel, may regulate actin polymerization which is crucial for several biological processes, including macrophage function. May also regulate macrophage function through regulation of the peroxisome proliferator activated receptor signaling pathway. Finally, it is also involved in the cellular response to IL13/interleukin-13. In addition to its role in the immune and inflammatory responses, may play a role in epithelial wound healing in the cornea maybe through production of lipoxin A4. May also play a role in endoplasmic reticulum stress response and the regulation of bone mass.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...