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Fzd8 (NM_008058) Mouse Untagged Clone
CAT＃: MC220409
Fzd8 (untagged) - Mouse frizzled homolog 8 (Drosophila) (Fzd8), (10ug)
CNY 7,536.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | Fz8 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC220409 representing NM_008058
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTGGGGTTACCTGTTGGAAGTGACCTCGCTCCTAGCCGCCTTGGCGGTGCTACAGCGCTCTAGCG GCGCTGCCGCGGCTTCGGCCAAGGAGCTGGCGTGCCAAGAGATCACGGTGCCGTTGTGCAAAGGCATCGG TTACAACTACACTTACATGCCCAACCAGTTCAACCACGACACGCAAGATGAGGCGGGCCTAGAGGTGCAC CAGTTTTGGCCGCTGGTGGAGATACAGTGCTCCCCGGACCTCAAGTTCTTTCTGTGTAGCATGTACACGC CCATCTGCCTGGAGGACTACAAGAAGCCTCTGCCGCCTTGTCGCTCTGTGTGTGAACGCGCCAAGGCCGG CTGCGCGCCGCTCATGCGCCAGTACGGCTTTGCTTGGCCTGACCGCATGCGCTGCGATCGGTTGCCGGAG CAGGGCAACCCGGACACTCTGTGCATGGACTACAACCGCACCGACCTCACCACGGCCGCGCCCAGCCCAC CGCGCCGCCTGCCTCCGCCGCCTCCTCCCGGCGAGCAGCCGCCCTCTGGCAGCGGCCACAGCCGCCCGCC AGGGGCCAGGCCCCCACATCGTGGCGGCAGCAGTAGGGGCAGCGGGGACGCGGCGGCTGCGCCCCCTTCG CGCGGCGGGAAGGCGAGGCCCCCTGGTGGCGGCGCTGCTCCCTGCGAGCCGGGGTGCCAGTGCCGCGCGC CCATGGTGAGCGTGTCCAGCGAACGCCACCCGCTCTACAACCGCGTCAAGACCGGCCAGATCGCCAACTG TGCGCTGCCCTGCCACAACCCCTTCTTTAGCCAGGATGAGCGCGCCTTCACCGTCTTCTGGATCGGCCTG TGGTCGGTGCTCTGCTTCGTCTCCACCTTCGCCACTGTCTCTACCTTCCTCATCGATATGGAGCGCTTTA AGTACCCGGAACGGCCCATCATATTCCTCTCCGCCTGTTACCTCTTCGTGTCTGTCGGGTACCTGGTGCG CCTGGTGGCAGGACATGAGAAAGTGGCCTGCAGCGGCGGCGCTCCGGGTGCTGGCGGAGCTGGGGGTGCG GGCGGCGCGGCGGCGGCTGGCGCAGGGGCAGCGGGAGCGGGGGCGAGCAGCCCGGGCGCGCGCGGCGAGT ACGAGGAGCTGGGTGCAGTCGAGCAGCATGTTCGCTATGAAACCACTGGCCCCGCGCTGTGCACGGTGGT CTTTCTCCTTGTCTACTTTTTTGGCATGGCCAGCTCCATCTGGTGGGTAATCCTGTCGCTCACGTGGTTC TTGGCAGCTGGCATGAAGTGGGGTAATGAGGCCATAGCAGGCTACTCGCAGTACTTCCACCTGGCCGCGT GGCTTGTGCCCAGCGTCAAGTCCATCGCGGTGCTGGCGCTCAGCTCCGTAGACGGCGACCCGGTGGCGGG CATCTGCTACGTGGGCAACCAGAGCCTTGACAACCTACGCGGCTTTGTGCTGGCGCCACTGGTTATCTAC CTCTTCATTGGGACTATGTTTCTGTTAGCTGGCTTCGTGTCGCTGTTCCGAATCCGTTCAGTCATCAAGC AGCAAGGAGGCCCAACTAAGACACACAAGCTAGAAAAACTCATGATCCGCTTGGGCCTCTTCACCGTGCT CTACACGGTGCCCGCTGCCGTCGTTGTCGCCTGCCTTTTCTATGAGCAGCACAACCGACCGCGCTGGGAG GCCACGCACAACTGCCCATGCCTTCGGGACCTGCAACCGGACCAGGCTCGCAGGCCCGATTACGCGGTCT TCATGCTCAAGTACTTCATGTGCCTAGTAGTGGGCATCACATCGGGCGTGTGGGTCTGGTCCGGCAAGAC TCTGGAGTCCTGGCGCGCGTTGTGCACTAGGTGCTGCTGGGCCAGCAAGGGCGCTGCAGTAGGCGCGGGC GCTGGAGGCAGCGGCCCTGGGGGCAGTGGACCCGGGCCCGGCGGAGGTGGGGGACACGGCGGAGGCGGGG GATCCCTCTACAGCGACGTCAGTACCGGCCTGACGTGGCGGTCTGGCACGGCCAGCTCTGTATCTTACCC TAAGCAAATGCCATTGTCCCAGGTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_008058 |
| Insert Size | 2058 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_008058.2, NP_032084.2 |
| RefSeq Size | 3346 bp |
| RefSeq ORF | 2058 bp |
| Locus ID | 14370 |
| UniProt ID | Q61091 |
| Gene Summary | Receptor for Wnt proteins. Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalsomes (By similarity). The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Coreceptor along with RYK of Wnt proteins, such as WNT1.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG223304 | Fzd8 (tGFP-tagged) - Mouse frizzled homolog 8 (Drosophila) (Fzd8), (10ug) |
CNY 9,128.00 |
|
| MR223304 | Fzd8 (Myc-DDK-tagged) - Mouse frizzled homolog 8 (Drosophila) (Fzd8) |
CNY 7,528.00 |
|
| MR223304L3 | Lenti ORF clone of Fzd8 (Myc-DDK-tagged) - Mouse frizzled homolog 8 (Drosophila) (Fzd8) |
CNY 8,740.00 |
|
| MR223304L4 | Lenti ORF clone of Fzd8 (mGFP-tagged) - Mouse frizzled homolog 8 (Drosophila) (Fzd8) |
CNY 8,740.00 |
