Prdm8 (NM_029947) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4930565F05Rik; PFM5
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC220521 representing NM_029947 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGATTCAGGCATCCAGAGAGGCATCTGGGATGGAGATGCCAAGGCTGTCCAACAGTGTCTGACAG ACATTTTTACCAGTGTATATACAACCTGCGACATCCCAGAGAACGCCATATTCGGTCCCTGTGTACTGAG CCATACTTCCCTGTATGACAGCATAGCCTTCGTAGCCCTCAAGTCCACGGACAAGAGAACAGTACCTTAC ATCTTCCGGGTAGACACTTCGGCGGCAAATGGTTCCTCAGAAGGTCTCATGTGGCTGCGGCTGGTCCAAT CAGCCCGAGATAAGGAAGAACAGAATCTCGAAGCTTATATAAAAAATGGACAGCTGTTCTACCGCTCTCT CCGCAGGATTGCCAAAGATGAAGAGTTACTAGTTTGGTACGGGAAAGAACTGACTGAATTGCTCTTGCTC TGCCCGTCTAGAGCTCACAAAATGAACGGGTCGTCTCCTTACACATGCCTGGAATGCAGCCAACGTTTCC AGTTTGAGTTCCCCTATGTGGCACATCTGCGATTCCGCTGCCCCAAGAGACTTCACAGCACTGATGCGAA TCCCCAAGACGAGCAAGGGGGCGGCTTGGGCACTAAGGACCACGGCGGCGGCGGCGGTGGTAAAGAGCAA CAGCAGCAGCAACAGCAGCAGCAACAGGAGGCGCCGTTGATCCCGGGCCCCAAGTTCTGCAAAGCCGGCC CCATACACCACTACCCCGCGTCATCCCCGGAGGCGAGCAACCCGCCGGGCTCCGCGGGTGCCGGCAGCGC CAAGCCGTCCACGGACTTCCACAACTTGGCTCGGGAACTGGAAAACTCCCGGGGAAGCAGCAGTTGCGTA GCGGCCCCGGGCGTCGGCAGTGGCGGCAGCGGCCACCAGGAGGCGGAGCTGAGTCCCGACGGCGTCGCCA CCGGCGGCTGCAAAGGCAAGAGGCGGTTCCCAGAGGAGGCGGCGGCGGAGGGCGGCGGCGCGGGGCTGGC GGGCGGCCGTGCGCGCTTCTCCGAGAGGCCGCTGGCGACCTCCAAGGAGGAGTTGGTGTGCACGCCGCAG CAGTACCGCGCCGCCGGCAGCTACTTCGGCCTGGAGGAGAACGGCCGGCTCTTCGCGCCGCCCAGCCCGG AGACTGGCGAGGCGAAGCGCAGCGCCTTCGTGGAGGTGAAGAAGGCGGGCCGCGCGGTGGGCTTGCAGGA GGAGGCGGCGGCGACAGACGGCGCAGGGGGCACGGCCGAGGACCCCGACGCGGGAGGCGGTGTCGCCGGC GGCGGCAGCAACGGCTCGTCCACCCCCGCGGCGGGGTCGCCTGGGGCCCCCGAGAAGTTGCTGGCTCCGC GTCCCGGAGGCTCTCTGCCCGGACGGCTGGAGGGCGGGAGCCCCGCGCGCGGCAGCGCCTTCACCTCGGT GTCGCAGCTGGGCGGCGGTGGCGGCGCGGGGACCGCGGGGACTGCGGGGGGCTCGGGGGGCGGCCAAACG GCCGCATCGGACGAGCGGAAGAGCGCCTTCTCGCAGCCCGCGCGCTCCTTCTCGCAGCTGTCCCCGTTGG TCCTCGGCCAGAAGCTGGGCGCTCTCGAGCCGTGTCACCCGGGAGACGGCGTGGGTCCCACCAGACTCTA CCCGGCCGCCGCCGATCCGCTGGCTGTGAAGCTGCAGGGGGCAGCGGACCTGAACGGAGCCTGCGGGCCC CTGGCGAGCGGCGGTGGCGGTGGCTTGCCCAAGCAGAGCCCCTTCCTCTACGCCACCGCCTTCTGGCCCA AGAGCTCGGCTGCGGCGGCAGCGGCGGCGGCGGCCGCCGCCGGGCCCCTGCAGCTGCAACTGCCCTCCGC GCTCACCCTGCTGCCGCCCTCCTTCACTTCGCTGTGTCTGCCCGCGCAGAACTGGTGCGCCAAATGCAAC GCCTCCTTCCGCATGACCTCCGACCTGGTGTACCATATGCGGTCTCACCACAAAAAGGAGTATGCCATGG AGCCCCTGGTGAAGCGGCGGCGGGAGGAGAAACTCAAGTGCCCCATTTGCAACGAGTCCTTCAGGGAGCG TCACCACCTGTCCCGGCACATGACCTCGCATAATTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_029947
Insert Size	2067 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_029947.2, NP_084223.2
RefSeq Size	3119 bp
RefSeq ORF	2067 bp
Locus ID	77630
UniProt ID	Q8BZ97
Gene Summary	Probable histone methyltransferase, preferentially acting on 'Lys-9' of histone H3 (PubMed:19646955). Histone methyltransferase activity has not been confirmed in other species. Involved in the control of steroidogenesis through transcriptional repression of steroidogenesis marker genes such as CYP17A1 and LHCGR (PubMed:19646955). Forms with BHLHE22 a transcriptional repressor complex controlling genes involved in neural development and neuronal differentiation (PubMed:22284184). In the retina, it is required for rod bipolar and type 2 OFF-cone bipolar cell survival (PubMed:26023183).[UniProtKB/Swiss-Prot Function]

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