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Jph2 (NM_021566) Mouse Untagged Clone
CAT＃: MC220586
Jph2 (untagged) - Mouse junctophilin 2 (Jph2), transcript variant 1, (10ug)
CNY 5,104.00
CNY 9,030.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 1110002E14Rik; JP-2; Jp2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC220586 representing NM_021566
Red=Cloning site Blue=ORF TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGGGGGCCGCTTTGACTTTGATGATGGCGGGGCGTATTGTGGGGGCTGGGAAGGGGGAAAGGCAC ACGGGCACGGACTGTGCACCGGCCCCAAGGGCCAGGGTGAATACTCGGGCTCCTGGAATTTTGGCTTTGA AGTGGCAGGCGTCTATACCTGGCCCAGTGGGAATACCTTTGAGGGATATTGGAGCCAGGGCAAACGACAT GGGCTTGGCATAGAGACCAAGGGGCGCTGGCTCTACAAGGGGGAGTGGACGCATGGCTTTAAGGGGCGCT ACGGAATCCGGCAGAGCACAAACAGTGGTGCCAAGTACGAGGGCACTTGGAATAACGGCCTACAGGACGG CTATGGCACGGAGACCTACGCAGACGGAGGAACCTATCAAGGCCAATTCACCAACGGCATGCGCCATGGC TACGGTGTGCGCCAAAGCGTGCCCTACGGGATGGCAGTGGTGGTGCGTTCTCCGCTGCGCACTTCTCTGT CCTCGCTGCGCAGCGAGCACAGCAATGGAACGGTGGCTCCGGACTCACCGGCGGCAGATGGGCCCATGCT GCCTTCGCCCCCAGTGCCGCGCGGTGGTTTCGCGCTCACTCTGCTGGCCACAGCAGAGGCCGCGCGACCC CAAGGGCTGTTCACGCGTGGCACACTGCTGGGTCGGCTGCGACGCTCAGAATCACGCACATCACTGGGCA GCCAGCGGAGCCGCTTGAGCTTTCTCAAGAGCGAGCTGAGTTCCGGAGCCAGCGATGCCGCATCCACTGG CAGCCTGGCCGAGGGCGCTGAGGGCCCCGACGACGCGGCTGCGCCCTTCGATGCCGACATCGACGCCACC ACCACGGAAACCTACATGGGCGAGTGGAAGAACGACAAGCGCTCGGGCTTCGGCGTGAGCGAGCGTTCCA GCGGCCTGCGCTACGAGGGCGAGTGGCTGGACAACCTGCGCCACGGCTACGGCCGCACCACGCTGCCCGA CGGCCACCGCGAGGAGGGCAAGTACCGCCACAATGTGCTGGTCAAGGGCACCAAGCGCCGCGTGCTGCCG CTCAAGAGCAGCAAGGTCCGCCAGAAGGTGGAGCACGGGGTGGAGGGCGCCCAGCGCGCAGCAGCCATCG CGCGCCAGAAGGCCGAGATTGCCGCCTCCAGGACAAGCCATGCCAAAGCCAAGGCAGAGGCAGCAGAACA GGCTGCCCTGGCTGCCAACCAGGAGTCCAACATCGCCCGTACATTGGCCAAGGAGCTGGCTCCAGACTTC TACCAGCCAGGTCCGGAGTATCAGAAGCGTCGGCTGCTCCAGGAGATCCTGGAGAACTCTGAGAGCTTGC TGGAGCCCCCAGAGCGGGGTCTGGGCACCGGCCTCCCGGAGCGGCCCCGGGAAAGCCCGCAGCTGCATGA GCGCGAGACCCCGCAGCCCGAGGGCGGACCCCCGTCTCCGGCCGGGACGCCCCCGCAACCCAAGAGGCCC CGGCCCGGAGCGTCAAAGGACGGCCTGCTGAGTCCAGGCTCCTGGAACGGGGAGCCCGGCGGAGAGGGCA GCCGGCCCGCCACGCCGTCGGATGGCGCCGGTCGTCGCAGCCCCGCGCGCCCCGCCTCGGAGCACATGGC CATCGAGGCGCTGCAGCCGCCGCCCGCGCCCTCGCAGGAGCCCGAGGTAGCAATGTACCGCGGCTACCAT AGCTACGCCGTGCGCACCGGGCCACCCGAGCCTCCGCCCTTGGAGGATGAGCAGGAGCCCGAGCCGGAGC CCGAGCCCGAGGTCCGGCGATCCGACTCGGCGCCCCCGTCCCCCGTCTCCGCCACCGTCCCGGAGGAGGA GCCCCCTGCGCCGCGAAGCCCGGTGCCTGCCAAGCAAGCCACTCTGGAGCCCAAGCCCATCGTCCCCAAA GCAGAGCCCAAGGCCAAGGCGCGCAAGACAGAGGCCCGAGGACTGAGCAAGGCCGGTGCCAAGAAGAAGG GCCGTAAGGAAGTGGCGCAGGCGAAGGAGGCCGAGGTGGAGGTGGAGGAGGTACCCAACACCGTCCTCAT CTGTATGGTGATCTTGCTGAACATCGGCCTGGCTATCCTATTTGTTCACCTCCTGACTTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-RsrII |
| ACCN | NM_021566 |
| Insert Size | 2091 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_021566.1, NP_067541.1 |
| RefSeq Size | 2681 bp |
| RefSeq ORF | 2091 bp |
| Locus ID | 59091 |
| UniProt ID | Q9ET78 |
| Gene Summary | Junctophilin-2: Membrane-binding protein that provides a structural bridge between the plasma membrane and the sarcoplasmic reticulum and is required for normal excitation-contraction coupling in cardiomyocytes (PubMed:10949023, PubMed:19095005, PubMed:21339484). Provides a structural foundation for functional cross-talk between the cell surface and intracellular Ca(2+) release channels by maintaining the 12-15 nm gap between the sarcolemma and the sarcoplasmic reticulum membranes in the cardiac dyads (PubMed:10949023, PubMed:19095005, PubMed:21339484). Necessary for proper intracellular Ca(2+) signaling in cardiac myocytes via its involvement in ryanodine receptor-mediated calcium ion release (PubMed:10949023, PubMed:19095005, PubMed:21339484). Contributes to the construction of skeletal muscle triad junctions (PubMed:10949023).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG220411 | Jph2 (tGFP-tagged) - Mouse junctophilin 2 (Jph2), (10ug) |
CNY 6,696.00 |
|
| MR220411 | Jph2 (Myc-DDK-tagged) - Mouse junctophilin 2 (Jph2), transcript variant 1 |
CNY 5,096.00 |
|
| MR220411L3 | Lenti ORF clone of Jph2 (Myc-DDK-tagged) - Mouse junctophilin 2 (Jph2), transcript variant 1 |
CNY 7,700.00 |
|
| MR220411L4 | Lenti ORF clone of Jph2 (mGFP-tagged) - Mouse junctophilin 2 (Jph2), transcript variant 1 |
CNY 7,700.00 |
