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Appl1 (NM_145221) Mouse Untagged Clone
CAT＃: MC220835
Appl1 (untagged) - Mouse adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (Appl1), (10ug)
CNY 9,120.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 2900057D21Rik; 7330406P05Rik; AI585782; AW209077; BB022931; C88264; DIP13 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC220835 representing NM_145221
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGGGATCGACAAGCTGCCCATTGAAGAGACCCTGGAGGACAGTCCGCAGACAAGGTCTTTACTTG GTGTATTTGAAGAAGATGCTACTGCTATTTCAAATTATATGAACCAATTGTATCAAGCTATGCATCGGAT TTATGATGCACAGAATGAATTGAGTGCAGCAACACACCTGACTTCGAAACTTTTGAAAGAATATGAAAAA CAGCGTTTTCCTTTGGGGGGTGATGATGAAGTTATGAGCTCTACTTTGCAGCAGTTTTCCAAGGTCATAG ATGAGCTTAGTTCTTGTCATGCAGTACTTTCAACTCAACTTGCTGATGCCATGATGTTCCCCATTTCCCA GTTTAAAGAAAGAGATCTGAAAGAAATATTGACACTAAAGGAGGTATTTCAAATTGCTAGTAATGATCAT GATGCCGCAATTAATAGGTACAGTCGGTTGTCAAAAAAGAGAGAAAATGACAAGGTAAAATATGAAGTTA CAGAGGATGTATATACTTCCAGGAAAAAACAACACCAGACCATGATGCATTATTTTTGTGCACTAAATAC TCTTCAGTACAAGAAGAAAATAGCTTTGTTAGAACCTCTACTGGGATACATGCAGGCTCAGATAAGTTTC TTTAAGATGGGTTCTGAAAATCTCAATGGACAACTGGAAGAATTCTTAGCTAACATTGGAACAAGTGTAC AGAATGTTCGAAGAGAAATGGATGGTGATGTAGAGACCATGCAGCAGACAATAGAAGACTTGGAGGTAGC CAGTGACCCTTTATATCTGCCTGACCCGGATCCCACCAAGTTTCCCATCAATCGAAATTTAACCCGAAAG GCTGGATACCTAAATGCTAGGAATAAAACAGGCTTGGTGTCATCTACCTGGGATAGACAGTTTTACTTCA CGCAGGGTGGAAACTTAATGAGTCAAGCCCGTGGAGATGTGGCAGGAGGCCTAGCCATGGACATAGACAA CTGTTCAGTGATGGCTGTGGACTGTGAAGACAGACGATACTGTTTTCAGATCACTTCTTTTGATGGAAAA AAATCTTCAATATTGCAAGCAGAGAGTAAAAAAGACCATGAAGAGTGGATCTGTACAATAAACAACATAT CTAAACAAATATATTTGAGTGAAAATCCAGAGGAAACTGCTGCACGAGTGAATCAGTCTGCTCTAGAAGC TGTCACTCCTTCCCCATCTTTCCAGCAGAGACACGAGAGCCTGCGTCCAGGAGGACAATCTCGGCCACCA ACAGCTCGAACCAGCAGTTCAGGATCCTTGGGATCAGAGTCTACAAATTTGGCTGCCCTCTCTCTAGATT CTCTTGTTGCTCCGGATACACCAATACAGTTTGATATAATTTCTCCTGTGTGTGAAGATCAGCCTGGCCA GGCAAAAGCCTTTGGTCAAGGAGGCAGGCGTACAAATCCATTTGGAGAATCTGGAGGAAGCACAAAATCA GAAACAGAAGACTCTATTCTTCATCAGTTATTTATTGTCCGATTCCTTGGTTCTATGGAGGTAAAGTCAG ATGACCATCCAGATGTTGTCTATGAAACAATGCGCCAGATCTTAGCTGCTCGGGCCATCCACAACATCTT TCGTATGACAGAATCACATTTATTAGTCACTTGTGACTGCTTGAAGTTAATTGATCCGCAGACCCAAGTT ACAAGGCTCACATTTCCATTGCCGTGTGTAGTTCTGTATGCTACGCACCAGGAAAATAAACGACTTTTTG GATTTGTTCTCCGGACATCAGGCGGAAGAAGTGAGAGTAATTTGTCATCAGTCTGCTATATATTTGAATC AAACAATGAGGGAGAAAAGATTTGTGATTCTGTTGGACTGGCAAAACAGATAGCTTTGCATGCTGAACTG GATCGTAGGGCATCAGAAAAACAAAAAGAAATAGAGAGAGTAAAAGAGAAACAGCAGAAAGAGCTCAGCA AACAGAAACAGATTGAGAAGGACTTGGAAGAACAGAGTCGGTTGATAGCTGCCTCCAGTAGACCAAACCA GGCTGGTAGTGAGGGCCAGCTTGTTCTTAGCAGTAGCCAATCAGAGGAGAGTGATTTGGGAGAAGAAGGG AAAAAAAGAGAGTCGGAAGCATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_145221 |
| Insert Size | 2124 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_145221.2, NP_660256.1 |
| RefSeq Size | 6961 bp |
| RefSeq ORF | 2124 bp |
| Locus ID | 72993 |
| UniProt ID | Q8K3H0 |
| Gene Summary | Multifunctional adapter protein that binds to various membrane receptors, nuclear factors and signaling proteins to regulate many processes, such as cell proliferation, immune response, endosomal trafficking and cell metabolism (By similarity) (PubMed:25328665, PubMed:25568335, PubMed:27219021). Regulates signaling pathway leading to cell proliferation through interaction with RAB5A and subunits of the NuRD/MeCP1 complex (By similarity). Functions as a positive regulator of innate immune response via activation of AKT1 signaling pathway by forming a complex with APPL1 and PIK3R1 (PubMed:25328665). Inhibits Fc-gamma receptor-mediated phagocytosis through PI3K/Akt signaling in macrophages (PubMed:25568335). Regulates TLR4 signaling in activated macrophages (PubMed:27219021). Involved in trafficking of the TGFBR1 from the endosomes to the nucleus via microtubules in a TRAF6-dependent manner. Plays a role in cell metabolism by regulating adiponecting and insulin signaling pathways (By similarity). Required for fibroblast migration through HGF cell signaling (PubMed:26445298). Positive regulator of beta-catenin/TCF-dependent transcription through direct interaction with RUVBL2/reptin resulting in the relief of RUVBL2-mediated repression of beta-catenin/TCF target genes by modulating the interactions within the beta-catenin-reptin-HDAC complex (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG210175 | Appl1 (tGFP-tagged) - Mouse adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (Appl1) |
CNY 6,370.00 |
|
| MR210175 | Appl1 (Myc-DDK-tagged) - Mouse adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (Appl1) |
CNY 5,176.00 |
|
| MR210175L3 | Lenti ORF clone of Appl1 (Myc-DDK-tagged) - Mouse adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (Appl1) |
CNY 7,700.00 |
|
| MR210175L4 | Lenti ORF clone of Appl1 (mGFP-tagged) - Mouse adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (Appl1) |
CNY 7,700.00 |
