Mapk6 (NM_027418) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2610021I23Rik; D130053K17Rik; Erk3; Mapk4; Mapk63; Prkm4; Prkm6
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC220972 representing NM_027418 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGAGAAATTCGAAAGTCTCATGAACATTCATGGCTTTGATCTGGGCTCTAGGTACATGGACTTAA AACCATTGGGCTGCGGAGGCAATGGCTTGGTCTTTTCTGCTGTAGACAATGACTGTGACAAAAGAGTAGC CATCAAGAAAATTGTCCTCACCGATCCCCAGAGTGTCAAACATGCTCTCCGTGAAATCAAAATTATTCGA AGACTTGACCACGATAACATTGTGAAAGTGTTTGAAATTCTTGGTCCCAGTGGAAGCCAGTTAACAGACG ATGTGGGCTCTCTCACGGAGCTGAATAGCGTCTACATTGTTCAGGAGTATATGGAGACAGACTTGGCGAA TGTGCTGGAGCAGGGCCCTTTACTGGAAGAGCATGCCAGGCTTTTCATGTATCAGCTGCTACGTGGGCTC AAATATATCCATTCTGCAAACGTACTGCACAGAGATCTCAAGCCAGCTAATCTTTTCATTAACACTGAAG ACTTGGTGCTGAAGATAGGTGACTTTGGCCTGGCACGGATCATGGATCCTCATTATTCCCATAAGGGTCA TCTTTCTGAAGGATTGGTTACCAAATGGTACAGATCTCCACGGCTTTTACTTTCTCCTAATAATTACACT AAAGCCATTGACATGTGGGCTGCAGGCTGCATCTTTGCTGAGATGCTGACTGGTAAAACCCTCTTTGCAG GTGCACATGAACTTGAACAGATGCAGCTGATCCTCGACTCCATCCCTGTTGTGCACGAGGAGGATCGGCA GGAGCTTCTCAGCGTGATTCCAGTTTACATTAGAAACGACATGACTGAGCCACACAGACCGCTCACTCAG CTGCTGCCGGGGATCAGCCGAGAAGCACTGGATTTCCTGGAGCAGATTCTGACTTTCAGCCCCATGGACC GGCTGACAGCTGAGGAAGCCCTTTCCCATCCTTACATGAGCATCTACTCCTTCCCGACGGATGAGCCCAT CTCAAGCCACCCTTTCCACATAGAGGACGAAGTGGACGACATTTTGCTTATGGATGAAACACACAGTCAC ATTTATAACTGGGAAAGGTACCACGATTGTCAGTTCTCGGAGCATGACTGGCCTATTCATAACAACTTTG ATATCGATGAGGTGCAGCTTGACCCAAGAGCTCTGTCTGACGTCACCGATGAAGAAGAAGTCCAAGTTGA TCCTCGAAAATACTTGGATGGAGACCGAGAGAAGTATCTAGAGGATCCCGCCTTCGACACCAGCTACTCT GCTGAACCCTGCTGGCAGTACCCAGATCACCACGAGAACAAGTACTGCGATCTGGAGTGTAGCCACACCT GTAACTACAAAACAAGATCATCACCATACTTAGATAACCTGGTGTGGAGGGAGAGCGAGGTTAACCATTA CTATGAGCCCAAGCTTATTATAGATCTTTCCAACTGGAAAGAGCAAAGTAAAGAAAAATCCGATAAGAGA GGCAAGTCCAAGTGTGAGAGGAACGGGTTGGTCAAGGCGCAGATCGCGCTAGAGGAAGCATCCCAGCAGC TGGCTGAGAGGGAGAGGGGCCAAGGCTTCGACTTTGACTCCTTCATCGCGGGGACCATTCAGCTCAGTGC CCAGCATCAGTCTGCTGACGTAGTTGACAAGTTAAACGACTTGAATAGCTCAGTGTCCCAGCTAGAATTG AAAAGCCTGATATCCAAGTCAGTCAGCCGAGAAAAGCAAGAAAAGGGAAGGGCTAACCTGGCCCAGCTGG GAGCCTTGTACCAGTCCTCCTGGGACAGCCAGTTTGTGAGTGGCGGGGAGGAGTGCTTCCTCATCAGTCA GTTTTGTTGTGAGGTCAGGAAGGACGAGCATGCGGAGAAGGAGAACACCTACACCAGCTATTTGGACAAG TTTTTTAGCAGGAAGGAGGATTCCGAAATGCTAGAAACTGAGCCAGTGGAGGAAGGGAAGCGTGGGGAGA GAGGCCGTGAGGCAGGGCTTCTGAGCGGCGGTGGGGAGTTCCTCCTGAGCAAGCAGCTGGAGTCCATAGG CACCCCGCAGTTCCACAGCCCAGTGGGGTCCCCACTCAAGTCCATCCAGGCCACCTTGACACCTTCCGCT ATGAAATCTTCCCCTCAAATCCCTCACAAGACATACAGCAGCATTCTGAAACATCTGAACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_027418
Insert Size	2163 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_027418.2, NP_081694.1
RefSeq Size	4209 bp
RefSeq ORF	2163 bp
Locus ID	50772
UniProt ID	Q61532
Gene Summary	Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK3/MAPK6 is phosphorylated at Ser-189 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6. May promote entry in the cell cycle.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) represents the longer transcript. Variants 1 and 2 encode the same protein.

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