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Ccnt2 (NM_028399) Mouse Untagged Clone
CAT＃: MC221106
Ccnt2 (untagged) - Mouse cyclin T2 (Ccnt2), (10ug)
CNY 9,310.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 2900041I18Rik; C81304; CycT2; CycT2a; CycT2b |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC221106 representing NM_028399
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTCGGGCCGTGGAGCTTCTTCTCGCTGGTTCTTTACTCGGGAACAGCTGGAGAACACGCCGAGCC GCCGCTGCGGAGTGGAAGCGGATGAAGAGCTGTCGCATCGCCAGCAGGCGGCCAACCTCATCCAGGACAT GGGGCAACGTCTCAATGTCTCTCAGCTTACAATAAACACTGCGATTGTTTATATGCACAGGTTTTATATG CACCATTCCTTCACCAAATTCAACAGAAATATAATATCACCTACTGCATTGTTTTTGGCTGCAAAAGTGG AAGAGCAGGCTCGAAAGCTTGAACATGTTATCAAAGTAGCACACGCTTGTCTTCATCCCCTAGAGCCACT CCTGGACACAAAATGTGATGCTTATCTTCAGCAGACTCAAGAACTGGTTTTACTTGAAACCATAATGCTA CAAACACTGGGTTTTGAGATCACCATTGAACACCCACACACAGATGTGGTGAAATGCACCCAGTTAGTAA GAGCAAGCAAGGATTTGGCACAGACATCCTATTTCATGGCTACCAACAGTTTGCATCTTACGACCTTCTG TCTTCAGTACAAACCCACAGTGATAGCATGTGTATGCATTCATTTGGCTTGCAAATGGTCCAATTGGGAG ATTCCTGTGTCAACAGATGGAAAGCACTGGTGGGAGTATGTGGACCCCACGGTTACCCTAGAGCTGCTAG ATGAACTAACACATGAGTTTCTACAAATATTGGAGAAAACGCCTAGTAGGTTGAAGAGGATTCGAAACTG GAGGGCTATGGCTAAGAAACCAAAAGTAGATGGACAAGTATCAGAGACACCACTGCTTGGTTCATCTTTG GTCCAGAATTCTATTTTAGTAGATAGTGTCACTGGTGTACCTGCCAACCCAAGCTTTCAGAAACCATCAA CATCAACGTTCCCTGCTCCAATACCTCTAAATTCAGGAAGTACTTCTGTTCAAGATAGCCGTGCATCTGA TAATTTATCAGTGCTAGCAGCAGGAATGCCCAGTACCTCATACAGTTTGTCATCACACCAAGAGTGGCCC CAGCATCCAGATTCAGCCAGGACAGACCCAGTGTACACACAGAAACAGGAGGCCACTCTCTCTGGGAGCC AGTACATAAGCTTCCAGCAGGGACCTTCAATGGCACTGCATTCTGGATTACATCACAGGCCGGACAAAGT TGCTGATCATTCATCAGCTAAGCAAGAATATACTCACAAAGCTGGGAGCAGTAAACACCATGGTCCCATA CCTGCTACTCCTGGAATGCTTCCTCAGAAAATGTCTTTAGATAAGTACAGAGAGAAGCGGAAGCTGGAAA CCCTTGACGTGGACACAAGGGATCACTATCTAGCTGCCCATGCAGAACAGCAGCACAAGCATGGGCCGGC TCAGGCAGTCACGGGCACTTCTGTCACTTCTCCCATCAAGATGAAGCTCCCCCTCACCAACTCGGACAGG CCTGAGAAACACGTGGCAGAGAAAAAGGAGAGGAGTGGATCACTGAAGCTCCGCATTCCCATCCCCCCGC CTGACAAAGGTCCCAGCAAAGAGGAGCTGAAGATGAAAATCAAAGTGGCATCCTCGGAAAGACACAGCTC TTCTGATGAAGGCAGTGGGAAGAGCAAGCACTCAAGCCCACACATCAGCAGAGACCACAAGGAGAAGCAC AAGGAGCACCCTGCCAACCGCCACCACAGCAGCCACAAGTATTTGCACATGCACAGTGGAGGCAGTAAGC ACACAGCGGATGGAATGCCTCCCACGGTGCTCAGGAGTCCTGTGGGCCTGGGCCCTGAAGGCGTCTCCTC CGCCTCCAGTGCAAGGAAGAAGCTGCATAGCAGCGAAGCCTCCCACAACCACCACTCCAAAATGAGCAAA AGTTCCAAAAGTGCAGGTAGTTCATCTAGTTCTTCCTCTGTTAAGCAGTATCTATCCTCTCACAGCTCTG TTTTTAACCATCCCTTACCCCCTCCTCCCCCTGTCACATACCAGGTGGGCTACGGACATCTCAGCACCCT CGTGAAACTGGACAAGAAACCAGTGGAGCCCCACGGTCCTGAGGCCAATCACGAGTACAGTACAAGCAGC CAGCATATGGACTACAAAGACACATTCGACATGCTGGACTCGCTGCTAAGTGCCCAAGGAATGAATATGT AA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_028399 |
| Insert Size | 2172 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_028399.1, NP_082675.1 |
| RefSeq Size | 3514 bp |
| RefSeq ORF | 2172 bp |
| Locus ID | 72949 |
| UniProt ID | Q7TQK0 |
| Gene Summary | Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin T) complex, also called positive transcription elongation factor B (P-TEFB), which is proposed to facilitate the transition from abortive to production elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNAP II). The activity of this complex is regulated by binding with 7SK snRNA (By similarity). Plays a role during muscle differentiation; P-TEFB complex interacts with MYOD1; this tripartite complex promotes the transcriptional activity of MYOD1 through its CDK9-mediated phosphorylation and binds the chromatin of promoters and enhancers of muscle-specific genes; this event correlates with hyperphosphorylation of the CTD domain of RNA pol II (PubMed:16245309, PubMed:23060074, PubMed:12037670). In addition, enhances MYOD1-dependent transcription through interaction with PKN1 (By similarity). Involved in early embryo development (PubMed:19364821).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG210294 | Ccnt2 (tGFP-tagged) - Mouse cyclin T2 (Ccnt2) |
CNY 7,030.00 |
|
| MR210294 | Ccnt2 (Myc-DDK-tagged) - Mouse cyclin T2 (Ccnt2) |
CNY 5,296.00 |
|
| MR210294L3 | Lenti ORF clone of Ccnt2 (Myc-DDK-tagged) - Mouse cyclin T2 (Ccnt2) |
CNY 8,270.00 |
|
| MR210294L4 | Lenti ORF clone of Ccnt2 (mGFP-tagged) - Mouse cyclin T2 (Ccnt2) |
CNY 8,270.00 |
