Gp1ba (NM_010326) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	GPIba; GPIbalpha
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC221152 representing NM_010326 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCTCCTCATCTTGCTCTTCCTGCTGCCAAGTCCCTTGCATTCCCAGCACACTTGTAGTATCTCCA AAGTGACCAGCCTGCTGGAAGTAAACTGTGAGAACAAGAAGCTGACAGCACTGCCTGCAGACCTGCCAGC AGACACAGGCATCCTCCACCTGGGCGAGAACCAACTGGGTACCTTCTCCACAGCATCACTGGTGCATTTC ACTCACCTCACTTATCTGTACCTGGATAGATGTGAGCTGACGAGCCTGCAGACCAATGGTAAACTGATAA AGCTGGAAAACCTGGACTTATCCCATAATAACCTGAAAAGCCTGCCCTCCCTAGGATGGGCATTGCCTGC ACTCACTACTCTAGACGTCTCCTTCAACAAGTTGGGCTCACTGTCTCCTGGTGTCCTGGATGGCCTAAGC CAACTCCAAGAGCTCTACTTGCAGAACAATGATCTTAAGAGTCTGCCCCCCGGGCTCTTGTTGCCAACGA CCAAGCTGAAGAAACTCAATCTGGCCAACAACAAATTGCGTGAGCTGCCCTCTGGGCTCCTAGATGGGCT GGAGGATCTTGATACCCTCTACCTTCAAAGGAACTGGCTTCGCACAATACCAAAGGGCTTCTTTGGGACC CTCCTCTTGCCTTTTGTTTTTCTCCACGCCAACAGCTGGTACTGTGATTGTGAAATCCTCTACTTCCGTC ACTGGCTCCAGGAAAATGCCAACAATGTCTACTTATGGAAGCAGGGTGTGGATGTCAAGGACACAACTCC TAACGTGGCCAGTGTACGATGTGCCAACTTGGACAATGCGCCTGTCTACTCCTACCCAGGGAAGGGCTGC CCTACTAGTAGTGGTGACACAGACTATGATGACTATGATGATATCCCTGACGTCCCTGCCACAAGAACTG AGGTCAAGTTCTCTACTAACACTAAAGTTCATACTACCCACTGGAGCCTACTCGCCGCAGCACCTTCTAC TTCTCAAGACAGCCAAATGATTTCTTTGCCTCCAACTCATAAACCCACTAAGAAACAATCTACATTCATC CACACACAGAGCCCAGGTTTCACCACACTCCCAGAGACCATGGAATCTAACCCAACCTTCTACAGTCTAA AACTCAATACTGTACTCATCCCCTCCCCAACCACCCTAGAGCCCACCTCCACCCAGGCTACCCCAGAGCC CAACATCCAACCAATGCTTACCACCTCCACCCTGACTACCCCAGAGCACAGCACCACCCCAGTGCCCACT ACTACCATCCTGACAACCCCAGAGCACAGCACCATCCCAGTGCCCACTACTGCCATCCTGACTACCCCAA AGCCCAGCACCATCCCAGTGCCCACTACTGCCACCCTGACTACCCTAGAGCCCAGCACCACCCCAGTGCC CACTACTGCCACCTTAACTACCCCAGAGCCCAGCACCACCCTGGTACCCACTACTGCCACCCTGACTACC CCAGAGCACAGCACCACCCCAGTGCCCACTACTGCCACTCTGACTACCCCAGAGCACAGCACCACCCCAG TGCCCACTACTGCCACCCTGACTACCCCAGAGCCCAGCACCACTTTAACTAATCTAGTGTCCACTATCTC CCCAGTGCTCACTACCACCCTGACCACTCCAGAGTCTACCCCAATTGAGACAATTTTAGAACAATTTTTT ACCACAGAGCTCACTTTACTCCCTACCTTAGAATCCACCACCACAATAATCCCAGAGCAGAACAGCTTTC TCAATCTCCCAGAGGTAGCTCTAGTAAGCTCTGATACTTCTGAAAGCAGTCCTTTTCTCAATTCTGACTT TTGCTGCTTCCTCCCCCTGGGCTTCTATGTCCTTGGTCTCCTCTGGCTCCTATTTGCCTCTGTGGTCCTC ATCTTGTTGCTGACCTGGACCTGGCATGTGACACCACATAGTCTGGACATGGAACAATCTGCTGCATTGG CCACAAGCACTCACACCACAAGCCTGGAGGTGCAGAGGGCAAGGCAAGTAACTATGCCCCGGGCCTGGCT GCTCTTCCTTCAAGGATCACTCCCTACTTTCCGGTCTAGCCTTTTCCTGTGGGTAAGGCCTAATGGGCGA GTGGGGCCTCTGGTAGCAGGACGGCGACCCTCAGCTCTGAGTCAGGGTCGTGGTCAGGACCTATTGGGCA CAGTGGGCATTAGGTACTCAGGCCATAGTCTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_010326
Insert Size	2205 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_010326.2, NP_034456.2
RefSeq Size	2732 bp
RefSeq ORF	2205 bp
Locus ID	14723
UniProt ID	O35930
Gene Summary	GP-Ib, a surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of vWF, which is already bound to the subendothelium.[UniProtKB/Swiss-Prot Function]

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