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Rnasel (NM_011882) Mouse Untagged Clone
CAT＃: MC221163
Rnasel (untagged) - Mouse ribonuclease L (2', 5'-oligoisoadenylate synthetase-dependent) (Rnasel), (10ug)
CNY 9,500.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | E230029I04Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC221163 representing NM_011882
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGACCCCGGATTATAACACACCTCAGGGTGGAACCCCATCAGCGGGAAGTCAGAGGACCGTTGTCG AAGATGATTCTTCGTTGATCAAAGCTGTTCAGAAGGGAGATGTTGTCAGGGTCCAGCAATTGTTAGAAAA AGGGGCTGATGCCAATGCCTGTGAAGACACCTGGGGCTGGACACCTTTGCACAACGCAGTGCAAGCTGGC AGGGTAGACATTGTGAACCTCCTGCTTAGTCATGGTGCTGACCCTCATCGGAGGAAGAAGAATGGGGCCA CCCCCTTCATCATTGCTGGGATCCAGGGAGATGTGAAACTGCTCGAGATTCTCCTCTCTTGTGGTGCAGA CGTCAATGAGTGTGACGAGAACGGATTCACGGCTTTCATGGAAGCTGCTGAGCGTGGTAACGCTGAAGCC TTAAGATTCCTTTTTGCTAAGGGAGCCAATGTGAATTTGCGACGACAGACAACGAAGGACAAAAGGCGAT TGAAGCAAGGAGGCGCCACAGCTCTCATGAGCGCTGCTGAGAAGGGCCACCTGGAAGTCCTGAGAATTCT CCTCAATGACATGAAGGCAGAAGTCGATGCTCGGGACAACATGGGCAGAAATGCCCTGATCCGTACTCTG CTGAACTGGGATTGTGAAAATGTGGAGGAGATTACTTCAATCCTGATTCAGCACGGGGCTGATGTTAACG TGAGAGGAGAAAGAGGGAAAACACCCCTCATCGCAGCAGTGGAGAGGAAGCACACAGGCTTGGTGCAGAT GCTCCTGAGTCGGGAAGGCATAAACATAGATGCCAGGGATAACGAGGGCAAGACAGCTCTGCTAATTGCT GTTGATAAACAACTGAAGGAAATTGTCCAGTTGCTTCTTGAAAAGGGAGCTGATAAGTGTGACGATCTTG TTTGGATAGCCAGGAGGAATCATGACTATCACCTTGTAAAGCTTCTCCTCCCTTATGTAGCTAATCCTGA CACCGACCCTCCTGCTGGAGACTGGTCGCCTCACAGTTCACGTTGGGGGACAGCCTTGAAAAGCCTCCAC AGTATGACTCGACCCATGATTGGCAAACTCAAGATCTTCATTCATGATGACTATAAAATTGCTGGCACTT CCGAAGGGGCTGTCTACCTAGGGATCTATGACAATCGAGAAGTGGCTGTGAAGGTCTTCCGTGAGAATAG CCCACGTGGATGTAAGGAAGTCTCTTGTCTGCGGGACTGCGGTGACCACAGTAACTTAGTGGCTTTCTAT GGAAGAGAGGACGATAAGGGCTGTTTATATGTGTGTGTGTCCCTGTGTGAGTGGACACTGGAAGAGTTCC TGAGGTTGCCCAGAGAGGAACCTGTGGAGAACGGGGAAGATAAGTTTGCCCACAGCATCCTATTATCTAT ATTTGAGGGTGTTCAAAAACTACACTTGCATGGATATTCCCATCAGGACCTGCAACCACAAAACATCTTA ATAGATTCCAAGAAAGCTGTCCGGCTGGCAGATTTTGATCAGAGCATCCGATGGATGGGAGAGTCACAGA TGGTCAGGAGAGACTTGGAGGATCTTGGACGGCTGGTTCTCTACGTGGTAATGAAAGGTGAGATCCCCTT TGAGACACTAAAGACTCAGAATGATGAAGTGCTGCTTACAATGTCTCCAGATGAGGAGACTAAGGACCTC ATTCATTGCCTGTTTTCTCCTGGAGAAAATGTCAAGAACTGCCTGGTAGACCTGCTTGGCCATCCTTTCT TTTGGACTTGGGAGAACCGCTATAGAACACTCCGGAATGTGGGAAATGAATCTGACATCAAAGTACGGAA ATGTAAAAGTGATCTTCTCAGACTACTGCAGCATCAGACACTTGAGCCTCCCAGAAGCTTTGACCAGTGG ACATCTAAGATCGACAAAAATGTTATGGATGAAATGAATCATTTCTACGAAAAGAGAAAAAAAAACCCTT ATCAGGATACTGTAGGTGATCTGCTGAAGTTTATTCGGAATATAGGCGAACACATCAATGAGGAAAAAAA GCGGGGAATGAAGGAGATACTTGGAGATCCTTCTCGTTATTTTCAGGAGACATTTCCAGATCTTGTAATC TATATCTACAAGAAACTAAAGGAAACAGAATACAGAAAACACTTCCCACAGCCTCCACCACGCCTCAGCG TTCCAGAGGCAGTGGGACCAGGGGGCATACAGAGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_011882 |
| Insert Size | 2208 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_011882.2, NP_036012.1 |
| RefSeq Size | 4758 bp |
| RefSeq ORF | 2208 bp |
| Locus ID | 24014 |
| UniProt ID | Q05921 |
| Gene Summary | Endoribonuclease that functions in the interferon (IFN) antiviral response. In INF treated and virus infected cells, RNASEL probably mediates its antiviral effects through a combination of direct cleavage of single-stranded viral RNAs, inhibition of protein synthesis through the degradation of rRNA, induction of apoptosis, and induction of other antiviral genes. RNASEL mediated apoptosis is the result of a JNK-dependent stress-response pathway leading to cytochrome c release from mitochondria and caspase-dependent apoptosis. Therefore, activation of RNASEL could lead to elimination of virus infected cells under some circumstances. In the crosstalk between autophagy and apoptosis proposed to induce autophagy as an early stress response to small double-stranded RNA and at later stages of prolonged stress to activate caspase-dependent proteolytic cleavage of BECN1 to terminate autophagy and promote apoptosis. Might play a central role in the regulation of mRNA turnover (By similarity). Cleaves 3' of UpNp dimers, with preference for UU and UA sequences, to sets of discrete products ranging from between 4 and 22 nucleotides in length (By similarity).[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG219460 | Rnasel (tGFP-tagged) - Mouse ribonuclease L (2' 5'-oligoisoadenylate synthetase-dependent) (Rnasel), (10ug) |
CNY 6,370.00 |
|
| MR219460 | Rnasel (Myc-DDK-tagged) - Mouse ribonuclease L (2', 5'-oligoisoadenylate synthetase-dependent) (Rnasel) |
CNY 5,384.00 |
|
| MR219460L3 | Lenti ORF clone of Rnasel (Myc-DDK-tagged) - Mouse ribonuclease L (2', 5'-oligoisoadenylate synthetase-dependent) (Rnasel) |
CNY 7,700.00 |
|
| MR219460L4 | Lenti ORF clone of Rnasel (mGFP-tagged) - Mouse ribonuclease L (2', 5'-oligoisoadenylate synthetase-dependent) (Rnasel) |
CNY 7,700.00 |
