Pomt1 (NM_145145) Mouse Untagged Clone
CAT#: MC221317
Pomt1 (untagged) - Mouse protein-O-mannosyltransferase 1 (Pomt1), (10ug)
CNY 9,690.00
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Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | AI505244 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC221317 representing NM_145145
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAGCCACTCTACGGGACTCGAAGAAACGCTCGGAGTCCTCCCGAGCTGGCTTTTCTGCAAAATGT TAAGATTTTTGAAACGGCCTCTAGTGGTGACTGTTGACATCAATTTGAACTTGGTAGCTCTGACTGGCCT GGGACTACTTACCCGACTATGGCAACTCTCCTACCCTCGGGCTGTGGTTTTCGATGAAGTATATTATGGG CAGTACATTTCCTTCTACATGAAGCGCATCTTCTTTCTGGATGACAGTGGGCCACCATTTGGCCACATGC TACTGGCCTTAGGAGGTTGGTTAGGGGGATTCGATGGTAACTTTCTGTGGAACCGAATTGGAGCAGAGTA CAGTAGCAATGTGCCTATATGGTCCTTACGCCTGCTGCCAGCGCTTGCCGGGGCCCTGTCAGTGCCCATG GCCTACCAGATAGTGCTAGAGCTCCACTTTTCCCACGGTGCTGCCATTGGAGCCGCCCTGCTGATGCTCA TTGAGAACGCCCTGATCACTCAGTCCAGGCTCATGCTGTTGGAGTCCATACTGATATTTTTTAACCTGTT GGCTGTGTTGTCCTATCTGAAGTTCTTCAACTCCCAGACACACAGCCCTTTCTCAGTGCACTGGTGGCTG TGGCTACTGCTGACCGGGGTCTCTTGTTCCTGTGCAGTTGGGATCAAATACATGGGGATTTTCACCTACC TGCTTGTGCTCGGCATTGCAGCTGTCCACGCGTGGAATCTGATCGGAGACCAGACCTTGTCAAATATGCG CGTGCTCAGTCACTTGCTCGCCAGAATCGTGGCTCTGCTGGTCGTCCCAGTCTTCCTGTACTTACTCTTC TTCTATGTCCACCTGATGCTGCTCTACCGCTCTGGGCCCCATGACCAAATCATGTCCAGTGCCTTCCAGG CCAGCTTGGAGGGAGGGCTTGCCCGCATCACCCAAGGCCAGCCCCTGGAGGTGGCCTTTGGTTCCCAGGT CACTCTGAAGAGCGTCTCTGGCAAACCCTTGCCCTGCTGGCTTCATTCGCACAAGAACACCTATCCCATG ATATATGAGAATGGCCGTGGCAGCTCCCACCAGCAACAGGTGACCTGTTATCCCTTCAAAGACATCAATA ACTGGTGGATCGTCAAGGATCCTGGGCGACACCAGCTGGTGGTAAACAACCCTCCCCGGCCTGTAAGACA TGGAGACATTGTACAGCTCGTTCACGGCATGACCACCCGCCTCCTTAACACGCACGATGTCGCAGCCCCA CTGAGCCCCCATTCTCAAGAAGTCTCCTGCTACATTGACTATAACATCTCCATGCCTGCCCAGAACCTCT GGAAACTGGACATTGTGAACAGAGAGTCCAACCGGGATACCTGGAAGACTATCTTGTCGGAAGTGCGCTT TGTACATGTGAACACATCCGCCATCTTGAAGCTGAGCGGGGCTCACCTCCCTGACTGGGGGTTCCGGCAG TTGGAGGTAGTTGGGGAGAAGTTGTCACCGGGCTACCACGAGAGCATGGTGTGGAATGTGGAAGAACACC GGTATGGCAAAAGCCATGAGCAGAAGGAGAGGGAGCTGGAGCTCCACTCGCCCACTCAGCTCGATATCAG CAGGAACCTCAGCTTCATGGCCAGATTCTCAGAGTTACAGTGGAAGATGCTGACGCTGAAGAATGAGGAC TTGGAACACCAGTACAGCTCCACCCCGCTGGAGTGGCTCACGCTGGACACCAACATCGCCTATTGGCTAC ATCCCAGGACCAGTGCTCAGATCCACTTGCTTGGGAACATTGTGATCTGGACTTCAGCCAGCCTTGCCAC AGTGGTCTACACTCTACTCTTCTTCTGGTACCTGCTCCGCCGGCGAAGGAGCATCTGTGACCTCCCTGAG GATGCCTGGTCCCGCTGGGTGCTGGCTGGAGCCCTGTGTACTGGCGGCTGGGCACTCAACTACCTGCCCT TCTTCCTGATGGAGAGGGTGCTCTTCCTCTACCACTACTTGCCGGCACTCACCTTCCAGATCCTGCTGCT CCCGATTGTCCTGCAGCACGCCAGCGACCATCTGTGCAGGTCCCAGCTGCAGAGGAATGTCTTCAGTGCC CTGGTTGTAGCATGGTATTCCTCCGCATGCCATGTGTCCAACATGCTACGCCCACTAACCTATGGGGACA CGTCACTCTCACCAGGCGAGCTCCGGGCCCTTCGCTGGAAAGACAGCTGGGATATTCTGATCCGAAAGTA A AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-RsrII |
ACCN | NM_145145 |
Insert Size | 2241 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_145145.1, NP_660127.1 |
RefSeq Size | 2838 bp |
RefSeq ORF | 2241 bp |
Locus ID | 99011 |
UniProt ID | Q8R2R1 |
Gene Summary | Transfers mannosyl residues to the hydroxyl group of serine or threonine residues. Coexpression of both POMT1 and POMT2 is necessary for enzyme activity, expression of either POMT1 or POMT2 alone is insufficient. Essentially dedicated to O-mannosylation of alpha-DAG1 and few other proteins but not of cadherins and protocaherins.[UniProtKB/Swiss-Prot Function] |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG210437 | Pomt1 (tGFP-tagged) - Mouse protein-O-mannosyltransferase 1 (Pomt1) |
CNY 7,220.00 |
|
MR210437 | Pomt1 (Myc-DDK-tagged) - Mouse protein-O-mannosyltransferase 1 (Pomt1) |
CNY 5,464.00 |
|
MR210437L3 | Lenti ORF clone of Pomt1 (Myc-DDK-tagged) - Mouse protein-O-mannosyltransferase 1 (Pomt1) |
CNY 8,460.00 |
|
MR210437L4 | Lenti ORF clone of Pomt1 (mGFP-tagged) - Mouse protein-O-mannosyltransferase 1 (Pomt1) |
CNY 8,460.00 |