Pomt1 (NM_145145) Mouse Untagged Clone

CAT#: MC221317

Pomt1 (untagged) - Mouse protein-O-mannosyltransferase 1 (Pomt1), (10ug)



  "NM_145145" in other vectors (4)

CNY 9,690.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AI505244
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC221317 representing NM_145145
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGAGCCACTCTACGGGACTCGAAGAAACGCTCGGAGTCCTCCCGAGCTGGCTTTTCTGCAAAATGT
TAAGATTTTTGAAACGGCCTCTAGTGGTGACTGTTGACATCAATTTGAACTTGGTAGCTCTGACTGGCCT
GGGACTACTTACCCGACTATGGCAACTCTCCTACCCTCGGGCTGTGGTTTTCGATGAAGTATATTATGGG
CAGTACATTTCCTTCTACATGAAGCGCATCTTCTTTCTGGATGACAGTGGGCCACCATTTGGCCACATGC
TACTGGCCTTAGGAGGTTGGTTAGGGGGATTCGATGGTAACTTTCTGTGGAACCGAATTGGAGCAGAGTA
CAGTAGCAATGTGCCTATATGGTCCTTACGCCTGCTGCCAGCGCTTGCCGGGGCCCTGTCAGTGCCCATG
GCCTACCAGATAGTGCTAGAGCTCCACTTTTCCCACGGTGCTGCCATTGGAGCCGCCCTGCTGATGCTCA
TTGAGAACGCCCTGATCACTCAGTCCAGGCTCATGCTGTTGGAGTCCATACTGATATTTTTTAACCTGTT
GGCTGTGTTGTCCTATCTGAAGTTCTTCAACTCCCAGACACACAGCCCTTTCTCAGTGCACTGGTGGCTG
TGGCTACTGCTGACCGGGGTCTCTTGTTCCTGTGCAGTTGGGATCAAATACATGGGGATTTTCACCTACC
TGCTTGTGCTCGGCATTGCAGCTGTCCACGCGTGGAATCTGATCGGAGACCAGACCTTGTCAAATATGCG
CGTGCTCAGTCACTTGCTCGCCAGAATCGTGGCTCTGCTGGTCGTCCCAGTCTTCCTGTACTTACTCTTC
TTCTATGTCCACCTGATGCTGCTCTACCGCTCTGGGCCCCATGACCAAATCATGTCCAGTGCCTTCCAGG
CCAGCTTGGAGGGAGGGCTTGCCCGCATCACCCAAGGCCAGCCCCTGGAGGTGGCCTTTGGTTCCCAGGT
CACTCTGAAGAGCGTCTCTGGCAAACCCTTGCCCTGCTGGCTTCATTCGCACAAGAACACCTATCCCATG
ATATATGAGAATGGCCGTGGCAGCTCCCACCAGCAACAGGTGACCTGTTATCCCTTCAAAGACATCAATA
ACTGGTGGATCGTCAAGGATCCTGGGCGACACCAGCTGGTGGTAAACAACCCTCCCCGGCCTGTAAGACA
TGGAGACATTGTACAGCTCGTTCACGGCATGACCACCCGCCTCCTTAACACGCACGATGTCGCAGCCCCA
CTGAGCCCCCATTCTCAAGAAGTCTCCTGCTACATTGACTATAACATCTCCATGCCTGCCCAGAACCTCT
GGAAACTGGACATTGTGAACAGAGAGTCCAACCGGGATACCTGGAAGACTATCTTGTCGGAAGTGCGCTT
TGTACATGTGAACACATCCGCCATCTTGAAGCTGAGCGGGGCTCACCTCCCTGACTGGGGGTTCCGGCAG
TTGGAGGTAGTTGGGGAGAAGTTGTCACCGGGCTACCACGAGAGCATGGTGTGGAATGTGGAAGAACACC
GGTATGGCAAAAGCCATGAGCAGAAGGAGAGGGAGCTGGAGCTCCACTCGCCCACTCAGCTCGATATCAG
CAGGAACCTCAGCTTCATGGCCAGATTCTCAGAGTTACAGTGGAAGATGCTGACGCTGAAGAATGAGGAC
TTGGAACACCAGTACAGCTCCACCCCGCTGGAGTGGCTCACGCTGGACACCAACATCGCCTATTGGCTAC
ATCCCAGGACCAGTGCTCAGATCCACTTGCTTGGGAACATTGTGATCTGGACTTCAGCCAGCCTTGCCAC
AGTGGTCTACACTCTACTCTTCTTCTGGTACCTGCTCCGCCGGCGAAGGAGCATCTGTGACCTCCCTGAG
GATGCCTGGTCCCGCTGGGTGCTGGCTGGAGCCCTGTGTACTGGCGGCTGGGCACTCAACTACCTGCCCT
TCTTCCTGATGGAGAGGGTGCTCTTCCTCTACCACTACTTGCCGGCACTCACCTTCCAGATCCTGCTGCT
CCCGATTGTCCTGCAGCACGCCAGCGACCATCTGTGCAGGTCCCAGCTGCAGAGGAATGTCTTCAGTGCC
CTGGTTGTAGCATGGTATTCCTCCGCATGCCATGTGTCCAACATGCTACGCCCACTAACCTATGGGGACA
CGTCACTCTCACCAGGCGAGCTCCGGGCCCTTCGCTGGAAAGACAGCTGGGATATTCTGATCCGAAAGTA
A


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-RsrII     
ACCN NM_145145
Insert Size 2241 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_145145.1, NP_660127.1
RefSeq Size 2838 bp
RefSeq ORF 2241 bp
Locus ID 99011
UniProt ID Q8R2R1
Gene Summary Transfers mannosyl residues to the hydroxyl group of serine or threonine residues. Coexpression of both POMT1 and POMT2 is necessary for enzyme activity, expression of either POMT1 or POMT2 alone is insufficient. Essentially dedicated to O-mannosylation of alpha-DAG1 and few other proteins but not of cadherins and protocaherins.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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