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Gphn (NM_172952) Mouse Untagged Clone
CAT＃: MC221466
Gphn (untagged) - Mouse gephyrin (Gphn), transcript variant 2, (10ug)
CNY 9,880.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 5730552E08Rik; AI662856; BC027112; C230040D23; geph; GPH; GPHRYN |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC221466 representing NM_172952
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACCGAGGGAATGATCCTCACCAACCACGACCATCAAATCCGTGTCGGAGTCCTCACAGTGAGTG ATAGCTGCTTCAGGAATCTTGCAGAAGACCGCAGTGGGATAAATCTAAAAGATCTTGTCCAAGATCCTTC TTTGTTGGGTGGGACTATATCGGCATACAAGATAGTACCAGATGAAATAGAAGAAATCAAGGAAACACTC ATAGACTGGTGTGATGAAAAGGAACTTAATTTAATATTAACAACTGGAGGAACGGGGTTTGCACCACGAG ATGTCACTCCAGAGGCCACAAAAGAAGTAATAGAGCGGGAAGCGCCAGGGATGGCCCTGGCAATGCTGAT GGGATCGCTCAATGTCACACCTCTGGGCATGCTCTCTAGACCAGTGTGTGGAATAAGGGGGAAAACTCTG ATAATTAACCTACCTGGTAGCAAGAAAGGATCTCAGGAATGCTTTCAGTTCATACTGCCAGCTCTACCTC ATGCCATTGACCTTTTACGTGATGCCATTGTAAAAGTAAAGGAGGTGCATGATGAACTTGAAGATTTACC TTCCCCACCACCTCCTCTCTCTCCACCTCCTACAACTAGCCCACATAAACAGACAGAAGACAAAGGAGTT CAGTGTGAAGAAGAGGAAGAAGAAAAGAAAGACAGTGGTGTAGCTTCAACAGAAGATAGTTCCTCATCAC ATATAACTGCAGCAGCTCTTGCTGCAAAGATTCCAGACTCCATCATTTCTCGTGGTGTTCAGGTGCTCCC ACGAGACACAGCCTCCCTTAGCACTACTCCTTCAGAATCGCCCCGTGCTCAGGCTACATCTCGCCTCTCT ACAGCTTCTTGTCCAACACCAAAACAAATTAGACGGCCGGATGAAAGCAAAGGAGTTGCTAGTAGAGTTG GATCCCTCAAAGCTCGGCTTCCGTCGTGCTCATCTACCTATAGTGTATCTGAGGTCCAGTCCAGGTGCAG CAGCAAGGAGAACATTCTAAGAGCCAGTCACAGTGCTGTAGATATCACCAAGGTGGCTAGAAGACATCGC ATGTCTCCTTTTCCCCTGACGTCTATGGACAAAGCCTTCATTACAGTCCTGGAGATGACTCCGGTGCTTG GTACAGAAATCATCAATTACCGAGATGGAATGGGGCGAGTCCTTGCTCAAGATGTATATGCAAAAGACAA CCTACCCCCATTCCCTGCATCAGTAAAAGATGGCTATGCTGTTCGAGCTGCTGATGGTCCAGGGGATCGT TTCATCATTGGGGAATCCCAAGCTGGTGAGCAGCCAACTCAGACAGTAATGCCAGGACAAGTGATGCGGG TTACAACAGGTGCTCCAATCCCCTGCGGTGCTGATGCAGTAGTGCAAGTTGAAGATACTGAACTCATCAG GGAATCAGATGATGGTACTGAAGAACTTGAAGTACGAATTCTGGTGCAAGCTCGGCCAGGCCAAGATATC CGACCCATCGGCCATGACATTAAGAGAGGGGAGTGTGTTTTGGCCAAAGGAACCCATATGGGCCCTTCAG AGATTGGTCTCCTTGCAACTGTGGGTGTCACAGAAGTGGAAGTTAATAAGTTTCCAGTGGTTGCCGTTAT GTCAACAGGGAATGAGCTGCTAAATCCTGAAGATGATCTCTTACCAGGAAAGATTCGGGACAGCAATCGA TCAACACTTCTAGCAACAATTCAGGAACATGGTTACCCAACAATCAACCTGGGAATTGTAGGAGACAACC CAGATGACTTACTCAATGCCTTGAATGAGGGTATCAGCCGTGCTGATGTCATCATCACGTCAGGAGGTGT GTCCATGGGGGAAAAGGACTATCTCAAGCAGGTGCTGGACATTGATCTTCATGCTCAGATCCATTTTGGC AGAGTTTTTATGAAGCCAGGCTTGCCAACAACATTTGCAACTTTGGATATTGATGGTGTAAGAAAAATTA TTTTTGCACTACCAGGGAATCCTGTATCAGCTGTGGTCACCTGCAACCTCTTTGTTGTACCTGCACTGAG AAAGATGCAGGGTATCTTGGATCCTCGGCCAACCATCATCAAAGCCAGGTTATCGTGTGATGTAAAACTG GACCCTCGCCCAGAATACCACCGGTGTATACTGACTTGGCATCACCAAGAACCACTGCCCTGGGCCCAGA GTACAGGTAATCAGATGAGCAGCCGTCTGATGAGCATGCGCAGTGCCAATGGATTATTGATGCTACCTCC AAAGACAGAGCAGTACGTGGAACTTCACAAAGGCGAGGTGGTAGATGTCATGGTCATTGGACGGCTATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_172952 |
| Insert Size | 2310 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_172952.3, NP_766540.2 |
| RefSeq Size | 3307 bp |
| RefSeq ORF | 2310 bp |
| Locus ID | 268566 |
| UniProt ID | Q8BUV3 |
| Gene Summary | Microtubule-associated protein involved in membrane protein-cytoskeleton interactions. It is thought to anchor the inhibitory glycine receptor (GLYR) to subsynaptic microtubules. Catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated. Subsequently, molybdate is inserted into adenylated molybdopterin and AMP is released.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks one coding exon and contains two additional coding exons compared to variant 1. The resulting protein (isoform 2) is shorter but has the same N- and C-termini compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG222215 | Gphn (tGFP-tagged) - Mouse gephyrin (Gphn) transcript variant 2, (10ug) |
CNY 8,460.00 |
|
| MR222215 | Gphn (Myc-DDK-tagged) - Mouse gephyrin (Gphn), transcript variant 2 |
CNY 7,700.00 |
|
| MR222215L3 | Lenti ORF clone of Gphn (Myc-DDK-tagged) - Mouse gephyrin (Gphn), transcript variant 2 |
CNY 9,600.00 |
|
| MR222215L4 | Lenti ORF clone of Gphn (mGFP-tagged) - Mouse gephyrin (Gphn), transcript variant 2 |
CNY 9,600.00 |
