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Ntrk1 (NM_001033124) Mouse Untagged Clone
CAT＃: MC221697
Ntrk1 (untagged) - Mouse neurotrophic tyrosine kinase, receptor, type 1 (Ntrk1), (10ug)
CNY 8,784.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | C80751; Tkr; trk; TrkA |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC221697 representing NM_001033124
Red=Cloning site Blue=ORF TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCGAGGCCAGCGGCTCGGGCAGCTGGGCTGGCATCGCCCGGCCGCGGGGCTAGGCAGTCTGATGA CTTCGTTGATGCTGGCCTGTGCCTCCGCCGCATCCTGTCGCGAGGTCTGCTGTCCCGTGGGCCCCTCGGG GCTGCGCTGCACCAGGGCAGGGTCCCTGGATACCCTCCGCGGCCTGCGGGGCGCCGGGAACCTGACGGAG CTCTACGTGGAAAACCAGCAGCACCTGCAACGCTTGGAGTTTGAGGACCTGCAGGGCCTGGGGGAGTTGA GAAGCCTAACCATCGTGAAGAGTGGCCTCCGCTTTGTGGCCCCAGATGCCTTCCGTTTCACCCCTCGGCT CAGTCACCTGAATCTGTCCTCCAATGCGTTGGAGTCCCTCTCCTGGAAAACTGTGCAGGGCCTCTCCCTA CAGGACCTGACCCTGTCAGGGAACCCACTGCATTGTTCCTGTGCCCTGTTCTGGCTCCAGCGTTGGGAGC AGGAAGGGCTGTGTGGTGTGCATACACAGACGCTTCATGACTCTGGGCCTGGAGACCAGTTCCTCCCACT GGGACACAACACCAGTTGTGGTGTACCCACAGTGAAGATCCAGATGCCCAATGACTCTGTGGAAGTGGGC GATGACGTGTTTCTGCAGTGCCAGGTGGAGGGGCTGGCCCTACAGCAGGCTGACTGGATCCTCACAGAGC TGGAAGGGGCAGCCACCGTGAAGAAATTTGGAGATCTGCCATCCCTGGGGCTGATTCTGGTCAATGTCAC CAGTGATCTCAACAAGAAGAATGTGACGTGCTGGGCAGAGAATGATGTGGGCCGGGCCGAGGTCTCTGTC CAAGTCAGCGTCTCCTTCCCAGCCAGTGTGCACCTGGGCCTAGCGGTGGAGCAGCATCATTGGTGCATCC CCTTCTCGGTGGACGGGCAGCCAGCACCGTCTCTGCGCTGGTTGTTCAACGGCTCTGTGCTCAACGAGAC CAGTTTCATCTTCACTCAGTTCTTGGAGTCTGCGCTGACTAATGAGACCATGCGGCACGGCTGCCTGCGC CTCAATCAGCCCACGCATGTCAACAACGGGAACTACACCCTGCTGGCGGCCAACCCCTACGGCCAGGCTG CCGCCTCCGTCATGGCTGCTTTTATGGACAACCCTTTTGAGTTCAACCCTGAGGACCCCATCCCTGTCTC CTTCTCGCCAGTGGACGGTAACAGCACATCAAGAGACCCAGTGGAGAAGAAAGATGAAACCCCTTTTGGG GTCTCTGTGGCTGTGGGCCTAGCCGTCTCTGCCGCCCTCTTCCTTTCTGCCCTCCTTCTTGTGCTCAACA AATGTGGACAGAGGAGCAAATTTGGGATCAACCGCCCTGCTGTATTAGCTCCAGAGGATGGGCTGGCCAT GTCCCTACACTTCATGACACTGGGTGGCAGTTCTCTTTCCCCTACTGAGGGCAAAGGCTCCGGACTCCAG GGCCACATCATGGAGAACCCACAGTACTTCAGTGATACCTGTGTCCATCACATCAAGCGCCAGGACATCA TTCTCAAGTGGGAGCTAGGGGAGGGAGCCTTTGGAAAGGTCTTTCTCGCTGAGTGCTACAACCTTCTGAA TGATCAGGACAAGATGCTCGTGGCTGTCAAGGCACTGAAGGAGGCATCCGAGAATGCTCGGCAGGACTTT CAGCGTGAGGCCGAGCTGCTCACCATGCTACAGCACCAGCACATCGTGCGCTTCTTTGGAGTCTGCACCG AGGGTGGCCCGTTGCTCATGGTCTTTGAGTACATGCGCCACGGGGACCTCAACCGTTTCCTCCGGTCCCA CGGACCTGATGCAAAGCTGCTGGCTGGTGGTGAGGATGTGGCTCCTGGTCCTCTGGGCCTTGGGCAGCTT CTGGCTGTGGCTAGCCAGGTGGCTGCTGGTATGGTATATCTAGCCAGCCTGCACTTTGTGCACCGGGATC TGGCCACACGCAACTGTCTGGTGGGTCAGGGACTAGTGGTGAAGATTGGAGACTTTGGCATGAGCAGAGA CATCTATAGCACAGACTATTACCGAGTGGGAGGTCGGACCATGCTGCCCATCCGCTGGATGCCACCCGAG AGCATCCTCTACCGCAAGTTCAGCACCGAGAGCGATGTGTGGAGCTTTGGGGTGGTGCTCTGGGAGATCT TCACCTATGGAAAGCAGCCCTGGTACCAGCTCTCCAACACTGAGGCGATCGAGTGTATCACGCAGGGCCG GGAGCTGGAGCGGCCGCGCGCCTGCCCTCCTGATGTCTACGCCATCATGCGAGGCTGCTGGCAGCGAGAA CCGCAGCAACGCCTCAGCATGAAGGATGTGCACGCGCGTCTGCAGGCCCTGGCACAGGCGCCACCCAGTT ACCTGGACGTTCTGGGCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001033124 |
| Insert Size | 2400 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001033124.1, NP_001028296.1 |
| RefSeq Size | 2606 bp |
| RefSeq ORF | 2400 bp |
| Locus ID | 18211 |
| UniProt ID | Q3UFB7 |
| Gene Summary | Receptor tyrosine kinase involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. High affinity receptor for NGF which is its primary ligand, it can also bind and be activated by NTF3/neurotrophin-3. However, NTF3 only supports axonal extension through NTRK1 but has no effect on neuron survival. Upon dimeric NGF ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades driving cell survival and differentiation. Through SHC1 and FRS2 activates a GRB2-Ras-MAPK cascade that regulates cell differentiation and survival. Through PLCG1 controls NF-Kappa-B activation and the transcription of genes involved in cell survival. Through SHC1 and SH2B1 controls a Ras-PI3 kinase-AKT1 signaling cascade that is also regulating survival. In absence of ligand and activation, may promote cell death, making the survival of neurons dependent on trophic factors.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG226042 | Ntrk1 (tGFP-tagged) - Mouse neurotrophic tyrosine kinase receptor type 1 (Ntrk1), (10ug) |
CNY 10,376.00 |
|
| MR226042 | Ntrk1 (Myc-DDK-tagged) - Mouse neurotrophic tyrosine kinase, receptor, type 1 (Ntrk1) |
CNY 8,776.00 |
|
| MR226042L3 | Lenti ORF clone of Ntrk1 (Myc-DDK-tagged) - Mouse neurotrophic tyrosine kinase, receptor, type 1 (Ntrk1) |
CNY 9,880.00 |
|
| MR226042L4 | Lenti ORF clone of Ntrk1 (mGFP-tagged) - Mouse neurotrophic tyrosine kinase, receptor, type 1 (Ntrk1) |
CNY 11,176.00 |
