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Mylk3 (NM_175441) Mouse Untagged Clone
CAT＃: MC221728
Mylk3 (untagged) - Mouse myosin light chain kinase 3 (Mylk3), (10ug)
CNY 10,260.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | D830007F02Rik; MLCK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC221728 representing NM_175441
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAGGAGTTTCAGAGGAGGACCCAGAGGGTCTGGCCCCCCAGGGTCTGCCAGCATTGGGCGGAGCCT GCTTAGCCACCATGGACAAAAAACTTAATGTGCTGACTGAGAAGGTCGACAGACTCTTGCATTTCCAAGA AGATGTCACAGAGAAGCTGCAGTGTGTGTGCCAAGGCATGGATCACCTGGAACAAGATCTGCATCGGCTG GAGGCCTCCCGGGAGTTGAGTCTGGCAGGGTCTGGCAGCACTCCCCCAACCACGGCTCAGGCCGCATGGC CTGAGGTCCTGGAGCTGGTGAGGGCTGTGCGGCAGGAGGGTGCCCAGCATGGTGCCAGGCTCGAAGCCCT CTTCAAGATGGTGGTGGCTGTGGACAGGGCTATTACTTTGGTAGGGTCCACATTCCAGAATTCCAAGGTG GCTGATTTCATCATGCAAGGAACCGTGCCCGGGAGAAAAGGCAGTCTGGCCGATGGCCCGGAGGAGAACA AGGAGCAAGCAGAAGTTGCTGGAGTGAAGCCAAACCATGTACTGACTACAGGAGGTGTGCAAGCTGACGC CTCTAGGACGCTGTGGGAAGAGAGCCAAAAGGAGGACATACCTGTGCGAACAGTGGAGGGGCTGCCTCTC ATCATCAATACGTCACTGAAGGGAGCTGACCTAACCCAGGCAGGAGCCTCACTGAGGCAGGGAGTTGAAG TTCTTGGCCCAGGCCAAGTACCCCTACCCACAGAAGCAGAATCCAGGCTTCCTGAGACAGCCAGTGAGAA CACTGGAGCCACCCTGGAATTGTCCGTAGCAATCGACAGAATCAGCGAGGTCCTCACTAGCCTCAAGATG TCACAAGGTGGTGGTCAAGAAACCTCATCCAGCAAGCCTGACTGTTGGCTTTCAGAAGAGGCCATGAGGC TGAGTTCAGGGCCTCTCCCTCAGCCCCTAGGACCACTAACTCCAGACAGTGACATTCACAGTGGTGATGC ACTTCCCAGGATCCCTATCAATATGCAAGAGATGGCTACTCCTGGGGAGTTGCTTGAGACCCAAAGTGGC AGTCCCATTGGCTCTGCAGAAGCTCCAGGCCTTGGGACTGTGTTAGAAGACCAGATCCCTAAAGGAGCCA GACCATTTCCACCCCTGCCAAAGAGGAGCAGCAACAATGGTGGCATGAGTGCAGAGGAGGAGATAGGGTC TGGGGCTGAGCCTATGAGAGGACCAAGCTTGGCTACAAGGGACTGGAGAGATGAGACTGTTGGGACCACA GACCTGCAGCAAGGCATAGACCCAGGAGCAGTGAGCCCTGAGCCTGGGAAGGACCACGCAGCCCAGGGCC CAGGAAGAACTGAAGCTGGAAGGCTATCTTCTGCTGCAGAGGCTGCCATTGTGGTTCTAGATGACAGCGC AGCACCCCCAGCCCCTTTTGAACACCGGGTAGTGAGCATCAAAGATACCCTGATCTCAGCAGGCTACACG GTATCCCAACATGAAGTCTTAGGAGGGGGTCGGTTTGGCCAGGTGCACAGGTGTACAGAGAGGTCTACAG GCCTTGCACTGGCAGCCAAGATCATCAAAGTGAAGAACGTAAAGGACCGGGAGGATGTGAAGAATGAGGT CAACATCATGAACCAGCTCAGCCACGTAAACTTGATCCAACTTTATGATGCGTTTGAGAGCAAGAGCAGC TTCACTCTGATCATGGAGTATGTGGATGGAGGCGAACTCTTTGACCGGATCACGGATGAGAAGTACCACC TCACTGAGTTGGATGTGGTCTTGTTCACGAGGCAGATCTGTGAGGGTGTGCATTACCTGCATCAGCACTA TATCCTGCACCTGGACCTCAAGCCTGAGAACATATTGTGTGTCAGCCAGACAGGGCATCAAATTAAGATC ATTGACTTTGGGCTGGCTAGAAGATACAAGCCTCGGGAGAAGCTAAAGGTGAACTTTGGTACTCCGGAGT TCCTGGCCCCAGAAGTTGTTAACTATGAGTTTGTGTCATTTCCAACAGACATGTGGAGTGTGGGAGTTAT CACCTACATGCTACTCAGTGGTTTGTCCCCATTTCTAGGGGAGACAGATGCAGAGACCATGAATTTTATT GTGAACTGCAGCTGGGATTTCGATGCTGATACCTTCAAAGGGCTGTCGGAGGAAGCCAAGGACTTTGTTT CCCGGTTACTGGTCAAAGAGAAGAGCTGTAGGATGAGCGCCACACAGTGCCTGAAACACGAGTGGTTAAG TCACCTGCCTGCCAAAGCCTCGGGCTCCAACGTTCGCCTCAGATCCCAACAACTGCTGCAGAAATATATG GCTCAGAGTAAATGGAAGAAACATTTCCACGTGGTGACTGCAGTCAACAGGCTACGGAAATTTCCAACGT GTCCCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_175441 |
| Insert Size | 2388 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_175441.5, NP_780650.2 |
| RefSeq Size | 3070 bp |
| RefSeq ORF | 2388 bp |
| Locus ID | 213435 |
| UniProt ID | Q3UIZ8 |
| Gene Summary | Kinase that phosphorylates MYL2 in vitro. Has been proposed to be calmodulin-dependent (PubMed:17885681), although MYL2 phosphorylation has also been observed in the presence or absence of calmodulin (PubMed:18202317). Promotes sarcomere formation in cardiomyocytes and increases cardiomyocyte contractility.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG225821 | Mylk3 (tGFP-tagged) - Mouse myosin light chain kinase 3 (Mylk3), (10ug) |
CNY 6,370.00 |
|
| MR225821 | Mylk3 (Myc-DDK-tagged) - Mouse myosin light chain kinase 3 (Mylk3) |
CNY 5,824.00 |
|
| MR225821L3 | Lenti ORF clone of Mylk3 (Myc-DDK-tagged) - Mouse myosin light chain kinase 3 (Mylk3) |
CNY 7,700.00 |
|
| MR225821L4 | Lenti ORF clone of Mylk3 (mGFP-tagged) - Mouse myosin light chain kinase 3 (Mylk3) |
CNY 7,700.00 |
