Pygb (NM_153781) Mouse Untagged Clone

CAT#: MC222017

Pygb (untagged) - Mouse brain glycogen phosphorylase (Pygb), (10ug)



  "NM_153781" in other vectors (4)

CNY 10,740.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms MGC36329
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC222017 representing NM_153781
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGAAGCCGTTGACGGACAGTGAGAGGCAGAAGCAGATCAGCGTGCGTGGCATCGCGGGGCTGGGCG
ACGTGGCCGAGGTGCGGAAGAGCTTCAACAGACACCTGCATTTCACGCTGGTCAAGGACCGCAATGTGGC
CACGCCCCGCGACTACTTCTTCGCTCTGGCACACACAGTTCGCGACCACCTAGTGGGCCGCTGGATCCGC
ACGCAGCAGCATTACTATGAGCGGGACCCCAAGCGCATCTATTACCTTTCCCTGGAATTCTACATGGGCC
GCACGCTGCAGAATACCATGGTGAACCTGGGCCTTCAGACTGCATGCGACGAAGCCACTTATCAGTTGGG
GTTGGACTTGGAAGAGCTAGAGGAGATAGAGGAGGATGCTGGTCTTGGGAATGGCGGCCTGGGGCGCCTG
GCAGCCTGCTTCCTTGATTCAATGGCCACCCTGGGTCTGGCTGCATACGGCTATGGAATCCGATATGAAT
TTGGGATTTTCAACCAAAAGATTGTCAATGGCTGGCAGGTTGAAGAAGCTGATGACTGGCTACGCTATGG
AAACCCCTGGGAGAAGGCACGGCCGGAGTACATGCTGCCGGTGCATTTCTACGGGAGGGTGGAGCACACC
CCTGACGGGGTCCTCTGGCTGGACACGCAGGTGGTACTCGCTATGCCCTACGACACCCCAGTGCCTGGGT
ATAAGAACAACACAGTCAACACCATGAGGCTCTGGTCTGCCAAGGCACCCAATGACTTCAAGCTGAAGGA
CTTCAACGTTGGGGACTACATCGAAGCTGTCCTGGATAGGAACTTGGCTGAGAACATCTCCAGGGTCCTG
TATCCCAATGACAATTTCTTCGAGGGGAAGGAGCTGCGGCTGAAGCAGGAGTACTTTGTGGTGGCTGCCA
CCCTCCAGGACATCATTCGAAGGTTCAAGTCTTCCAGGTTTGGCTGCCGGGACCCAGTGAGAACCTGTTT
CGAGACATTCCCAGACAAGGTAGCCATCCAGCTGAATGACACCCACCCCGCGCTCTCCATCCCTGAGCTC
ATGCGGATCCTGGTGGACGTGGAGAAGGTGGACTGGGATAAGGCCTGGGAGATCACCAAGAAGACCTGTG
CCTACACCAACCACACGGTGCTGCCCGAGGCCCTGGAGCGCTGGCCAGTGTCTATGTTTGAGAAGCTGCT
TCCGCGGCACCTGGAAATAATCTATGCCATCAACCAGAGGCACTTGGACCACGTGGCTGCCCTGTTTCCT
GGGGACGTGGACCGACTGAGGAGGATGTCCGTGATTGAGGAAGGGGACTGCAAGAGAATCAACATGGCAC
ACCTGTGTGTGATTGGGTCCCACGCTGTGAACGGGGTGGCAAGGATTCACTCTGAGATCGTGAAGCAGTC
TGTCTTTAAGGACTTTTACGAACTGGAGCCAGAGAAGTTCCAGAACAAGACAAATGGCATCACGCCCCGA
CGGTGGCTGCTGTTGTGTAACCCGGGGCTGGCAGAAATCATTGTAGAGAGAATCGGGGAAGGTTTCCTGA
CCGATCTGAGCCAACTGAAGAAGCTGCTGTCACTGGTTGATGATGAGGCCTTCATCAGAGACGTAGCCAA
GGTTAAGCAGGAAAACAAGCTGAAGTTCTCTGCCCAGCTAGAGAAGGAGTACAAGGTGAAGATCAACCCT
GCATCCATGTTTGATGTGCACGTGAAGAGGATCCACGAATATAAGCGACAGCTGCTCAACTGCCTACACA
TTATCACCCTGTACAACCGAATAAAGAAGGATCCAGCCAAGGCCTTTGTGCCCAGGACTGTTATGATTGG
GGGCAAGGCAGCTCCTGGTTACCACATGGCTAAGATGATCATCAAGCTGGTCACATCCATTGGAGATGTT
GTCAATCATGATCCAGTTGTGGGCGACAGGCTCAGAGTGATCTTCCTGGAGAACTACCGTGTGTCTTTAG
CTGAGAAAGTGATCCCAGCGGCTGACCTGTCCCAGCAGATCTCCACTGCGGGCACTGAAGCCTCGGGCAC
AGGCAACATGAAGTTCATGCTCAATGGAGCGCTCACCATCGGCACCATGGATGGTGCCAACGTGGAGATG
GCCGAGGAGGCCGGGGAGGAGAACCTCTTCATCTTTGGTATGCGGGTGGAAGATGTCGAGGCCCTGGACC
AGAAAGGGTACAATGCCAGAGAATTCTATGAGCGCCTGCCTGAGCTGAGGCAGGCTGTGGATCAGATCAG
CAGTGGCTTCTTTTCTCCCAAAGATCCAGACTGCTTCAAGGACGTGGTTAACATGCTGATGTACCACGAC
AGATTCAAGGTATTTGCAGACTATGAAGCCTACATCCAATGCCAGGCCCAGGTGGACCGTCTATACCGGA
ATTCCAAGGAGTGGACTAAAAAGGTGATCAGGAATATAGCCTGCTCTGGCAAGTTCTCCAGTGACCGGAC
CATCACTGAATACGCCCGGGAGATCTGGGGTGTGGAGCCCTCTGACCTGCAGATCCCACCCCCCAACCTC
CCCAAGGACTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_153781
Insert Size 2532 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_153781.1, NP_722476.1
RefSeq Size 3860 bp
RefSeq ORF 2532 bp
Locus ID 110078
UniProt ID Q8CI94
Gene Summary Glycogen phosphorylase that regulates glycogen mobilization. Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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