Nefm (NM_008691) Mouse Untagged Clone

CAT#: MC222041

Nefm (untagged) - Mouse neurofilament, medium polypeptide (Nefm), (10ug)



  "NM_008691" in other vectors (4)

CNY 10,830.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Nef3; NF-M; NF160; NF165; Nfm
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC222041 representing NM_008691
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCTACACGCTGGACTCGCTGGGCAACCCGTCCGCCTACCGGCGGGTCACCGAGACCCGCTCCAGCT
TCAGCCGCGTGAGCGGTTCCCCGTCCAGCGGCTTCCGCTCGCAGTCCTGGTCCCGCGGCTCGCCCAGCAC
CGTGTCCTCCTCCTACAAGCGCAGCGCGCTCGCCCCGCGTCTCGCCTACAGCTCGGCTATGCTCAGCTCG
GCCGAGAGCAGCCTCGACTTCAGCCAGTCCTCGTCGCTGCTCAACGGCGGCTCCGGCGGCGACTACAAAC
TGTCCCGCTCTAACGAGAAAGAGCAGCTGCAGGGGCTGAACGACCGCTTCGCCGGCTACATCGAGAAAGT
GCACTACTTGGAACAACAGAACAAGGAGATCGAAGCAGAGATCCAGGCACTGCGGCAGAAGCAGGCCTCG
CACGCCCAGCTGGGTGATGCTTACGACCAGGAGATCCGAGAGCTGCGCGCCACCCTCGAGATGGTGAACC
ACGAGAAGGCTCAAGTGCAGCTGGACTCCGATCACTTGGAGGAAGACATCCACCGGCTCAAGGAGCGCTT
CGAGGAGGAGGCGCGGCTGCGGGACGACACCGAGGCTGCCATTCGCGCGCTGCGCAAAGACATCGAAGAG
TCGTCGATGGTTAAGGTGGAGCTGGACAAGAAGGTGCAGTCGCTGCAGGATGAGGTGGCTTTCCTGCGGA
GCAATCACGAAGAGGAGGTGGCCGACCTGCTGGCTCAGATCCAGGCGTCGCACATCACGGTAGAGCGCAA
AGATTACCTGAAGACAGACATCTCCACGGCGCTGAAGGAGATCCGCTCCCAGCTCGAGTGTCACTCAGAC
CAGAACATGCACCAGGCCGAAGAGTGGTTCAAATGCCGCTACGCCAAGCTCACCGAGGCGGCCGAGCAGA
ACAAGGAGGCCATTCGCTCTGCCAAGGAAGAGATCGCCGAGTACCGGCGCCAGCTGCAGTCCAAGAGCAT
CGAGCTCGAGTCGGTGCGAGGCACTAAGGAGTCCCTGGAGCGGCAGCTCAGCGACATCGAGGAGCGCCAC
AACCACGACCTCAGCAGCTACCAGGACACCATCCAGCAGTTGGAAAATGAACTTCGGGGAACCAAGTGGG
AAATGGCTCGTCATTTGCGAGAATACCAGGATCTCCTTAACGTCAAGATGGCCCTGGACATCGAGATCGC
CGCGTACAGGAAACTCCTAGAGGGGGAAGAGACCAGATTTAGCACATTTTCAGGAAGCATCACCGGGCCT
CTGTACACACACCGACAGCCCTCAGTCACAATATCCAGTAAGATTCAGAAGACCAAAGTCGAGGCCCCCA
AGCTCAAGGTCCAACACAAATTTGTGGAGGAGATCATCGAAGAAACTAAAGTGGAAGATGAGAAGTCAGA
AATGGAAGAAACCCTCACAGCCATCGCAGAGGAGTTGGCAGCCTCCGCCAAAGAGGAGAAGGAAGAGGCC
GAAGAAAAGGAGGAGGAACCAGAAGCCGAAAAGTCTCCCGTGAAGTCTCCTGAGGCTAAGGAAGAGGAGG
AGGAAGGGGAAAAGGAGGAAGAAGAGGAAGGCCAGGAGGAAGAAGAGGAGGAAGATGAAGGTGTCAAGTC
AGACCAGGCAGAAGAGGGGGGATCTGAGAAGGAAGGCTCCAGTGAAAAAGATGAAGGTGAGCAGGAAGAA
GAAGAAGGAGAAACCGAGGCAGAAGGTGAAGGAGAGGAAGCAGAAGCTAAGGAGGAAAAGAAAATTGAGG
GAAAGGTTGAGGAAGTGGCTGTCAAGGAGGAAATCAAGGTCGAGAAGCCTGAGAAAGCCAAATCCCCTAT
GCCCAAATCACCCGTGGAAGAAGTAAAGCCAAAACCAGAGGCCAAGGCCGGGAAGGGTGAGCACAAGGAG
GAAGAGAAAGTTGAGGAAGAGAAGAAGGAAGTCACCAAAGAATCACCCAAGGAAGAGAAGGTGGAGAAAA
AGGAGGAGAAGCCAAAAGATGTTGCAGATAAAAAGAAGGCCGAGTCCCCGGTGAAAGAGAAGGCTGTGGA
GGAGGTGATCACCATCAGCAAGTCGGTAAAGGTGAGCCTAGAGAAAGACACCAAAGAGGAGAAGCTGCAG
CCGCAGGAGAAGGTGAAGGAGAAGGCAGAGGAGGAGGGGGGCAGTGAGGAGGAAGGGAGTGACCGTAGCC
CGCAGGAGTCCAAGAAGGAAGACATAGCTATCAATGGGGAGGTGGAAGGAAAAGAGGAGGAGGAGCAGGA
AACTCAGGAGAAGGGCAGTGGGCGGGAGGAGGAGAAAGGGGTGGTCACTAATGGCTTAGATGTGAGCCCT
GCAGAGGAGAAGAAAGGAGAGGATAGCAGTGATGATAAAGTGGTGGTCACCAAGAAGGTAGAAAAGATCA
CCAGCGAGGGGGGCGATGGTGCTACCAAATACATCACCAAATCTGTAACCGTCACTCAAAAGGTTGAAGA
GCATGAGGAGACCTTTGAGGAGAAGCTGGTCTCAACTAAAAAGGTAGAAAAGGTCACTTCACACGCCATA
GTCAAGGAAGTCACCCAGGGTGACTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_008691
Insert Size 2547 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_008691.2, NP_032717.2
RefSeq Size 3244 bp
RefSeq ORF 2547 bp
Locus ID 18040
UniProt ID P08553
Gene Summary Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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