Cul5 (NM_027807) Mouse Untagged Clone

CAT#: MC222170

Cul5 (untagged) - Mouse cullin 5 (Cul5), transcript variant 1, (10ug)



  "NM_027807" in other vectors (4)

CNY 10,930.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 4921514I20Rik; 8430423K24Rik; AI852817; C030032G03Rik; C330021I08Rik; VACM-1; VACM1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC222170 representing NM_027807
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGCTCTCTCGCGTGGGCAGGCCGGGGTGACCATGTAGCTGGAAAGCCCGAGGAAGCACGGCTGCCCG
GGACGAGCTCGGCGCTGACGGCACGCCGCTCCGGCGTCCCCCGCATCCCCCGCCGCGGCCTGCGGGGTCC
TGCTGGGAACCCCGGCCTCTCGAGGAGGCCTGGCCCCGAGCGCCGCCAAGTCTCGCCCCGTCTCGCGAGA
GTCCAAGTTGAGAACATGGCGACGTCTAATCTGTTAAAGAATAAAGGTTCTCTCCAGTTTGAAGACAAGT
GGGACTTCATGCATCCAATTGTTTTGAAGCTTTTACGCCAGGAATCTGTAACAAAACAGCAGTGGTTTGA
TCTATTTTCGGATGTACATGCTGTCTGTCTCTGGGATGATAAAGGCTCATCAAAAATTCATCAGGCTTTA
AAAGAAGATATTCTTGAGTTTATTAAGCAAGCACAGGCTCGTGTACTGAGCCATCAAGATGACACAGCTT
TGCTGAAGGCATATATTGTTGAATGGCGGAAATTCTTCACACAGTGTGATATTTTACCAAAACCTTTTTG
TCAATTAGAGGTGACTCTATTGGGTAAACAAAGCAGCAATAAAAAATCAAATATGGAAGACAGTATTGTT
CGAAAGCTCATGCTTGATACGTGGAATGAGTCGATTTTTTCAAATATAAAGAACAGACTCCAGGACAGTG
CAATGAAGCTGGTGCATGCTGAGAGATTAGGGGAAGCTTTTGATTCCCAGCTGGTCATCGGGGTGCGAGA
GTCCTATGTTAATCTTTGCTCCAACCCCGAGGACAAGCTTCAGATCTATAGGGATAATTTTGAGAAGGCA
TACTTGGACTCAACAGAGAGGTTTTATAGAACACAGGCACCCTCATATTTACAGCAAAATGGTGTGCAGA
ATTACATGAAATATGCAGATGCTAAATTAAAAGAAGAAGAAAAAAGAGCACTCCGATACTTAGAAACACG
ACGAGAGTGTAACTCTGTGGAAGCACTCATGGAATGCTGTGTAAATGCGCTGGTGACCTCCTTTAAAGAG
ACTATTTTAGCAGAATGCCAAGGCATGATCAAGCGAAATGAAACTGAAAAGTTACATTTGATGTTTTCCT
TGATGGACAAAGTTCCTAATGGGATAGAGCCGATGTTGAAGGACTTGGAGGAGCATATTATAAGTGCGGG
CCTAGCAGACATGGTGGCCGCAGCTGAAACCATCACTACTGACTCTGAGAAGTATGTGGAGCAATTACTT
ACACTGTTTAATAGATTCAGTAAACTGGTCAAAGAAGCTTTTCAGGATGATCCTCGTTTCCTTACTGCAA
GAGATAAGGCATATAAAGCAGTTGTTAATGATGCTACTATATTTAAACTTGAATTGCCTTTGAAGCAAAA
AGGAGTGGGGTTGAAAACTCAGCCTGAGTCAAAATGCCCCGAGTTGCTTGCCAATTACTGTGACATGTTG
TTAAGGAAAACGCCATTAAGCAAAAAACTAACATCTGAGGAGATTGAAGCAAAGCTTAAAGAAGTGCTCT
TGGTACTTAAATATGTACAAAACAAAGATGTTTTTATGAGGTATCACAAAGCTCATCTTACCCGACGTCT
CATATTGGACATCTCTGCTGATAGTGAGATTGAAGAAAACATGGTAGAGTGGCTAAGAGAAGTTGGTATG
CCAGCAGATTATGTGAACAAGCTTGCTAGAATGTTTCAGGACATAAAAGTATCTGAAGACTTGAACCAAG
CTTTTAAGGAAATGCACAAAAATAATAAGTTGGCATTACCAGCTGATTCCGTTAATATAAAGATTTTGAA
TGCTGGTGCTTGGTCTAGAAGCTCCGAGAAAGTCTTTGTCTCACTTCCTACTGAACTGGAGGATTTGATA
CCTGAAGTAGAAGAATTTTACAAAAAAAATCACAGTGGTAGAAAATTACACTGGCACCATCTCATGTCAA
ATGGAATTATAACATTTAAAAATGAAGTAGGTCAGTATGATTTGGAAGTAACCACGTTTCAGTTGGCTGT
GTTGTTTGCATGGAACCAAAGGCCTAGAGAGAAAATCAGCTTTGAAAATCTAAAACTTGCAACGGAACTC
CCAGATGCTGAACTTAGAAGGACTTTATGGTCTTTAGTAGCTTTTCCCAAGCTCAAACGGCAAGTTTTGT
TGTATGACCCTCAAGTCAACTCACCCAAAGATTTTACAGAAGGCACCCTCTTCTCAGTGAACCAGGACTT
CAGTCTCATAAAAAATGCAAAAGTACAGAAAAGGGGGAAAATCAATTTGATTGGACGCTTGCAGCTCACT
ACAGAACGAATGAGAGAAGAAGAAAATGAAGGGATAGTCCAACTAAGAATATTAAGAACCCAGGAAGCCA
TCATACAAATAATGAAAATGAGAAAGAAAATTAGCAATGCCCAGCTGCAGACTGAATTAGTAGAAATTCT
GAAAAACATGTTCCTGCCTCAGAAGAAGATGATAAAGGAGCAGATGGAGTGGCTGATTGAACACAGGTAC
ATCCGGAGGGACGAGGCCGACATCAACACCTTCATCTACATGGCCTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_027807
Insert Size 2568 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_027807.3, NP_082083.2
RefSeq Size 6023 bp
RefSeq ORF 2568 bp
Locus ID 75717
UniProt ID Q9D5V5
Gene Summary Core component of multiple SCF-like ECS (Elongin BC-Cullin 2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. ECS(SOCS1) seems to direct ubiquitination of JAK2. Seems to be involved in proteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. May form a cell surface vasopressin receptor.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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