Ago2 (NM_153178) Mouse Untagged Clone

CAT#: MC222199

Ago2 (untagged) - Mouse eukaryotic translation initiation factor 2C, 2 (Eif2c2), (10ug)



  "NM_153178" in other vectors (4)

CNY 10,930.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 1110029L17Rik; 2310051F07Rik; AI225898; AL022874; AW546247; Eif2c2; ENSMUSG00000072493; Gerp95
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC222199 representing NM_153178
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTACTCGGGAGCCGGCCCCGTTCTTGCTTCTCCTGCTCCGACAACATCACCCATCCCAGGATATGCCT
TCAAACCTCCACCTCGGCCGGACTTCGGCACCACCGGGAGAACAATCAAACTACAGGCCAATTTCTTTGA
AATGGACATCCCCAAAATTGACATCTATCACTATGAATTGGACATCAAACCTGAGAAATGCCCTCGGAGA
GTGAACAGGGAAATTGTGGAGCACATGGTCCAGCACTTTAAAACTCAGATCTTCGGGGACCGGAAGCCAG
TGTTTGATGGAAGGAAGAATCTGTACACAGCAATGCCCCTTCCGATCGGCAGGGACAAGGTGGAGCTGGA
GGTCACGCTCCCGGGAGAAGGCAAAGATCGCATCTTTAAGGTATCCATCAAGTGGGTGTCGTGCGTGAGC
CTGCAGGCGTTACACGATGCACTTTCGGGGCGGCTGCCCAGCGTCCCCTTCGAGACGATCCAGGCCCTGG
ACGTTGTCATGAGGCACTTACCATCCATGAGGTACACCCCTGTTGGCCGTTCCTTCTTCACTGCATCTGA
AGGCTGTTCCAACCCTCTGGGTGGGGGCAGAGAAGTGTGGTTTGGCTTCCATCAGTCCGTCCGACCTTCT
CTTTGGAAAATGATGCTGAATATTGATGTATCGGCAACAGCGTTTTACAAGGCACAGCCAGTGATCGAGT
TTGTTTGTGAAGTTTTGGATTTTAAAAGTATTGAAGAACAACAAAAACCTCTGACAGATTCCCAAAGGGT
AAAGTTTACCAAAGAAATCAAAGGTCTAAAGGTGGAGATAACGCACTGTGGTCAGATGAAGAGGAAGTAC
CGTGTCTGCAATGTGACCCGGCGGCCTGCCAGTCACCAAACGTTCCCACTGCAGCAGGAGAGTGGGCAGA
CAGTGGAGTGTACAGTGGCCCAGTACTTCAAGGACAGGCACAAGCTGGTTCTGCGCTACCCCCACCTCCC
GTGTTTACAAGTCGGACAGGAGCAGAAACACACCTACCTTCCTTTGGAGGTCTGTAACATAGTTGCTGGA
CAGAGATGTATAAAAAAATTAACAGACAATCAGACCTCAACCATGATCAGAGCAACTGCCAGGTCAGCAC
CTGATCGCCAAGAGGAGATCAGCAAACTGATGCGAAGTGCAAGTTTCAATACAGACCCATATGTTCGTGA
ATTTGGAATCATGGTGAAAGATGAGATGACAGATGTGACTGGGCGGGTTCTGCAGCCGCCCTCCATCCTC
TACGGGGGCAGGAACAAAGCAATTGCCACCCCTGTCCAGGGTGTGTGGGACATGCGAAACAAGCAGTTCC
ACACGGGCATCGAGATCAAGGTGTGGGCCATCGCCTGCTTCGCTCCCCAGCGCCAGTGTACAGAAGTCCA
TCTCAAGTCCTTCACAGAGCAACTGAGGAAGATCTCGAGAGATGCTGGGATGCCGATCCAGGGTCAGCCC
TGCTTCTGTAAATACGCACAGGGTGCAGACAGTGTGGAGCCCATGTTCCGACACCTGAAGAACACATACG
CTGGCCTCCAGCTGGTGGTGGTCATCCTGCCTGGCAAAACTCCTGTGTATGCGGAAGTCAAGCGTGTGGG
AGACACAGTGCTGGGGATGGCCACACAGTGCGTCCAGATGAAGAACGTGCAGAGGACCACGCCACAGACC
CTATCCAATCTCTGCTTAAAGATCAATGTCAAACTGGGAGGCGTCAACAACATCCTGCTGCCACAGGGCA
GGCCTCCAGTGTTCCAGCAACCTGTCATCTTCCTGGGAGCCGATGTCACCCACCCACCAGCTGGGGATGG
GAAGAAACCTTCTATTGCTGCCGTCGTGGGCAGCATGGACGCACACCCCAACCGCTACTGTGCCACCGTG
CGCGTGCAGCAGCACCGACAGGAGATCATCCAGGACCTGGCTGCCATGGTACGAGAGTTGCTCATTCAGT
TCTACAAGTCCACCCGCTTCAAGCCCACCCGCATCATCTTCTACCGCGATGGCGTCTCCGAGGGCCAGTT
CCAGCAGGTTCTCCACCATGAGCTCCTGGCCATCAGAGAGGCCTGCATCAAGCTGGAGAAGGACTATCAG
CCAGGAATCACGTTCATCGTGGTGCAGAAGCGGCACCACACACGCCTCTTCTGCACAGACAAAAATGAGC
GGGTTGGGAAGAGTGGGAACATTCCCGCAGGCACAACCGTGGACACGAAGATCACCCACCCCACTGAGTT
TGACTTCTACCTGTGCAGTCACGCCGGCATCCAGGGGACAAGCCGTCCTTCCCACTACCACGTGCTTTGG
GATGACAATCGTTTTTCTTCTGATGAGCTGCAGATTCTGACCTACCAGCTGTGTCACACCTACGTGCGCT
GCACACGCTCTGTGTCAATACCCGCGCCAGCTTACTATGCTCACCTGGTGGCCTTCCGAGCCAGGTACCA
CCTGGTGGATAAAGAACATGACAGCGCTGAAGGAAGCCATACCTCTGGGCAGAGCAACGGGCGAGATCAC
CAAGCGCTGGCCAAGGCGGTCCAGGTCCACCAGGACACACTGCGCACCATGTACTTTGCTTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_153178
Insert Size 2583 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_153178.4, NP_694818.3
RefSeq Size 8031 bp
RefSeq ORF 2583 bp
Locus ID 239528
UniProt ID Q8CJG0
Gene Summary Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3' UTR). Can also up-regulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3' UTR of the TNF (TNF-alpha) mRNA and up-regulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions. Regulates lymphoid and erythroid development and function, and this is independent of endonuclease activity.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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