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Trim71 (NM_001042503) Mouse Untagged Clone
CAT＃: MC222236
Trim71 (untagged) - Mouse tripartite motif-containing 71 (Trim71), (10ug)
CNY 10,930.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 636931; 2610206G21Rik; AL022943; Gm1127; lin-41; Lin41; mlin-41; mLin41 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC222236 representing NM_001042503
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTCGTTCCCGGAGACCGACTTCCAGATCTGCCTGCTGTGCAAGGAGATGTGCGGCTCGCCCGCGC CGCTCTCCTCCAACTCGTCCGCCTCGTCGTCGTCCTCGCAGACTTCCACGTCGTCGGCGGGCGGCGGCGG CCCCGGGGCGGCGGCGCGGCGTCTTCACGTCTTGCCCTGCCTGCACGCCTTCTGCCGGCCGTGCCTCGAG GCGCACCGGCTGCCCGCTCCCGGAGGCGCGGGCCCCGCCGAGGCGCTGAAGCTGCGCTGCCCCGTATGTG ACCAGAAGGTGGTCCTGGCCGAGGCGGCGGGCATGGACGCGCTGCCATCCTCCGCCTTCCTGCTCAGCAA CCTGCTGGACGCCGTGGTGGCCACCGCCGAAGAGCCGCCGCCCAAGAACGGGCGCGCGGGCGGCGGCCCC GGGGGCGCGGGCGGCCACAGCAACCACCGGCACCATGCGCACCACCCGGCCCAGCGCGCCGCCGCTCCAG CGCCGCAGCCGCCGCCCGGCCCGGCCGCCTCCCCCGGGTCGCTGCTGATGCGCCGGCCGCACGGCTGCAG CTCCTGCGACGAGGGCAACGCCGCCTCGTCGCGCTGCCTCGACTGCCAGGAACACCTGTGCGACAACTGT GTCCGCGCGCACCAGCGCGTCCGCCTCACCAAGGACCATTACATCGAGCGCGGCCCTCCGGGGCCTGCGG CCGCCTCCGCGGCACAGCAGCTGGGGCTCGGGCCGCCCTTCGCGGGCGCGCCCTTCTCCATTCTCTCGGT GTTCCCGGAGCGCCTCGGCTTCTGCCAGCACCACGACGATGAGGTCCTGCATCTCTACTGTGACACCTGC TCTGTCCCCATCTGCAGAGAGTGCACGCTGGGCCGCCACGGTGGCCACAGCTTCGCCTACCTCCAGGATG CCCTGCAGGACTCTCGGGCACTCACCATCCAGCTGCTGGCTGATGCCCAGCAGGGCCGCCAGGCTCTCCA GCTGAGCATCGAGCAGGCCCAGACTGTGGCAGAACAGGTGGAGATGAAGGCGAAGGTGGTCCAGTCTGAG GTCAAGGCCGTCACAGCTCGCCACAAGAAGGCCCTGGAGGATCGGGAGTGTGAGCTGTTGTGGAAGGTGG AGAAGATCCGGCAGGTGAAGGCGAAGTCTCTGTTCCTGCAGGTGGAGAAGCTGCGGCAGAGTCTCAGCAA GCTGGAGAGCACCATCAGCGCCGTGCAGCAGGTCCTGGAAGAGGGCCGCGCCCTGGACATCCTGCTGGCC CGAGACCGGATGCTGGCCCAGGTGCAGGAGCTCAAGACCATCCGAGGCCTCCTGCAGCCCCAAGAAGATG ACCGCATTATGTTCACGCCCCCTGACCAGGCCCTGTACCTCGCCCTCAAGTCTATTGGCTTTGTCAGCAG CGGGGCCTTCGCCCCGCTCACGAAGGCTACAGGAGATGGCATCAAGCGTGCCCTTCAGGGGAAGGTGGCC TCTTTCACTGTCATGGGCTACGACCACGATGGTGAGCCCCGCCATTCGGGAGGTGACCTGATGTCAGTTG TGGTCCTCGGCCCCGACGGAAACCTGTTTGGTGCAGAAGTGAGCGACCAGCAGAATGGTACTTACATAGT GAGCTACAGGCCCCAGCTTGAGGGTGAGCACTTGGTGTCGGTGACATTGTACAATCAGCATATAGAGAAC AGCCCCTTCAAGGTCGTGGTCAAGTCGGGCCGCAGCTACGTGGGCATCGGGCTCCCCGGGCTGAGCTTCG GCAGTGAAGGTGACGGGGAGGGCAAGCTCTGCAGGCCCTGGGGCGTGAGTGTTGATAAGGAGGGTTTCAT CATAGTGGCTGACCGAAGCAATAACAGAATCCAGGTGTTTAAGCCCTGTGGTTCCTTCCACCACAAGTTT GGTACCCTTGGGTCTCGTCCTGGGCAGTTTGACCGACCGGCTGGGGTGGCTTGTGACGCCTCCCGCAGGA TCATAGTGGCCGACAAAGACAATCATCGCATTCAGATCTTCACCTTCGAGGGCCAGTTCCTTCTTAAGTT TGGTGAGAAAGGCACCAAGAACGGGCAGTTTAACTACCCGTGGGATGTAGCAGTGAATTCTGAGGGCAAG ATCCTCGTTTCAGACACCCGGAATCATCGCATCCAGTTGTTCGGACCGGACGGGGTCTTCCTGAACAAGT ACGGCTTCGAGGGATCTCTCTGGAAGCACTTCGACTCTCCACGGGGCGTGGCTTTCAACAATGAAGGTCA CCTGGTAGTCACCGACTTCAACAACCACCGGCTTCTGGTTATTCACCCTGATTGCCAATCTGCCCGCTTC TTGGGTTCGGAGGGCTCGGGCAATGGGCAGTTCCTGCGACCACAGGGTGTGGCTGTGGACCAGGAAGGGA GAATCATCGTAGCCGATTCCAGAAATCACCGCGTGCAGATGTTTGAGGCCAATGGCAGCTTCCTGTGCAA GTTTGGTGCTCAAGGGAGTGGCTTCGGGCAGATGGACCGCCCCTCAGGTATCGCGGTCACCCCGGATGGA TTGATCGTCGTGGTGGACTTTGGCAACAATCGAATCCTCATCTTCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001042503 |
| Insert Size | 2568 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001042503.2, NP_001035968.1 |
| RefSeq Size | 5197 bp |
| RefSeq ORF | 2568 bp |
| Locus ID | 636931 |
| UniProt ID | Q1PSW8 |
| Gene Summary | E3 ubiquitin-protein ligase that cooperates with the microRNAs (miRNAs) machinery and promotes embryonic stem cells proliferation and maintenance (PubMed:19898466). Binds to miRNAs and associates with AGO2, participating in post-transcriptional repression of transcripts such as CDKN1A. Facilitates the G1-S transition to promote rapid embryonic stem cell self-renewal by repressing CDKN1A expression (PubMed:22735451). In addition, participates in post-transcriptional mRNA repression in a miRNA independent mechanism (PubMed:23125361). Required to maintain proliferation and prevent premature differentiation of neural progenitor cells during early neural development: positively regulates FGF signaling by controlling the stability of SHCBP1 (PubMed:22735451). Specific regulator of miRNA biogenesis. miRNA Binds MIR29A hairpin and postranscriptionally modulates MIR29A levels, which indirectly regulates TET proteins expression (By similarity).[UniProtKB/Swiss-Prot Function] |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG224725 | Trim71 (tGFP-tagged) - Mouse tripartite motif-containing 71 (Trim71), (10ug) |
CNY 8,270.00 |
|
| MR224725 | Trim71 (Myc-DDK-tagged) - Mouse tripartite motif-containing 71 (Trim71) |
CNY 6,264.00 |
|
| MR224725L3 | Lenti ORF clone of Trim71 (Myc-DDK-tagged) - Mouse tripartite motif-containing 71 (Trim71) |
CNY 9,410.00 |
|
| MR224725L4 | Lenti ORF clone of Trim71 (mGFP-tagged) - Mouse tripartite motif-containing 71 (Trim71) |
CNY 9,410.00 |
