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Epas1 (NM_010137) Mouse Untagged Clone
CAT＃: MC222307
Epas1 (untagged) - Mouse endothelial PAS domain protein 1 (Epas1), (10ug)
CNY 11,210.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | bHLHe73; HIF-2alpha; HIF2A; HLF; HRF; MOP2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC222307 representing NM_010137
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAGCTGACAAGGAGAAAAAAAGGAGCAGCTCAGAGCTGAGGAAGGAGAAATCCCGTGATGCCGCGA GGTGCCGGCGCAGCAAGGAGACGGAGGTCTTCTATGAGTTGGCTCATGAGTTGCCCCTGCCTCACAGTGT GAGCTCCCACCTGGACAAAGCCTCCATCATGCGCCTGGCCATCAGCTTCCTTCGGACACATAAGCTCCTG TCCTCAGTCTGCTCTGAAAATGAATCTGAAGCTGAGGCCGACCAGCAAATGGATAACTTGTACCTGAAAG CCTTGGAGGGTTTCATTGCTGTGGTGACCCAAGACGGTGACATGATCTTTCTGTCGGAAAACATCAGCAA GTTCATGGGACTTACTCAGGTAGAACTAACAGGACACAGCATCTTTGACTTCACTCATCCTTGCGACCAT GAGGAGATCCGTGAGAACCTGACTCTCAAAAACGGCTCTGGTTTTGGGAAGAAGAGCAAAGACGTGTCCA CCGAGCGTGACTTCTTCATGAGGATGAAGTGCACGGTCACCAACAGAGGCCGGACTGTCAACCTCAAGTC GGCCACCTGGAAGGTCCTGCACTGCACCGGGCAAGTGAGAGTCTACAACAACTGCCCCCCTCACAGTAGC CTCTGTGGCTCCAAGGAGCCCCTGCTGTCCTGCCTTATCATCATGTGTGAGCCAATCCAGCACCCATCCC ACATGGACATCCCCCTGGACAGCAAGACTTTCCTGAGCCGCCACAGCATGGACATGAAGTTCACCTACTG TGACGACAGAATCTTGGAACTGATTGGTTACCACCCCGAGGAGCTACTTGGACGCTCTGCCTATGAGTTC TACCATGCCCTGGATTCGGAGAACATGACCAAAAGTCACCAGAACTTGTGCACCAAGGGGCAGGTGGTAT CTGGCCAGTACCGGATGCTAGCCAAACACGGAGGATATGTGTGGCTGGAGACCCAGGGGACGGTCATCTA CAACCCCCGCAACCTGCAGCCTCAGTGTATCATGTGTGTCAACTATGTGCTGAGTGAGATCGAGAAGAAC GACGTGGTGTTCTCCATGGACCAGACCGAATCCCTGTTCAAGCCACACCTGATGGCCATGAACAGCATCT TTGACAGCAGTGACGATGTGGCTGTAACTGAGAAGAGCAACTACCTGTTCACCAAACTGAAGGAGGAGCC CGAGGAACTGGCCCAGTTGGCCCCCACCCCAGGAGATGCCATTATTTCTCTCGATTTCGGAAGCCAGAAC TTCGATGAACCCTCAGCCTATGGCAAGGCCATCCTTCCCCCGGGCCAGCCATGGGTCTCGGGGCTGAGGA GCCACAGTGCCCAGAGCGAGTCCGGGAGCCTGCCAGCCTTCACTGTGCCCCAGGCAGACACCCCAGGGAA CACTACACCCAGTGCTTCAAGCAGCAGTAGCTGCTCCACGCCCAGCAGCCCTGAGGACTACTATTCATCC TTGGAGAATCCCTTGAAGATCGAAGTGATTGAGAAGCTTTTCGCCATGGACACGGAGCCGAGGGACCCGG GCAGTACCCAGACGGACTTCAGTGAACTGGATTTGGAGACCTTGGCACCCTACATCCCTATGGACGGCGA GGACTTCCAGCTGAGCCCCATCTGCCCAGAGGAGCCGCTCATGCCAGAGAGCCCCCAGCCCACCCCCCAG CACTGCTTCAGTACCATGACCAGCATCTTCCAGCCGCTCACCCCGGGGGCCACCCACGGCCCCTTCTTCC TCGATAAGTACCCGCAGCAGTTGGAAAGCAGGAAGACAGAGTCTGAGCACTGGCCCATGTCTTCCATCTT CTTTGATGCTGGGAGCAAAGGGTCCCTGTCTCCATGCTGTGGCCAGGCCAGCACCCCTCTCTCTTCTATG GGAGGCAGATCCAACACGCAGTGGCCCCCGGATCCACCATTACATTTCGGCCCTACTAAGTGGCCTGTGG GTGATCAGAGTGCTGAATCCCTGGGAGCCCTGCCGGTGGGGTCATCGCAGTTGGAACCTCCGAGCGCCCC GCCTCATGTCTCCATGTTCAAGATGAGGTCTGCAAAGGACTTCGGGGCCCGAGGTCCATACATGATGAGC CCAGCCATGATCGCCCTGTCCAACAAGCTGAAGCTAAAGCGGCAGCTGGAGTATGAGGAGCAAGCCTTCC AAGACACAAGCGGGGGGGACCCTCCAGGCACCAGCAGTTCACACTTGATGTGGAAACGTATGAAGAGCCT CATGGGCGGGACCTGTCCTTTGATGCCTGACAAGACCATCAGTGCGAACATGGCCCCCGATGAATTCACC CAAAAATCTATGAGAGGCCTGGGCCAGCCACTGAGACACCTGCCACCTCCCCAGCCACCATCTACCAGGA GCTCAGGGGAGAACGCCAAGACTGGGTTCCCGCCACAGTGCTATGCCTCCCAGTTCCAGGACTACGGTCC TCCAGGAGCTCAAAAGGTGTCAGGCGTGGCCAGTCGACTGCTGGGGCCATCGTTCGAGCCTTACCTGTTG CCGGAACTGACCAGATATGACTGTGAGGTGAACGTGCCCGTGCCTGGAAGCTCCACACTCCTGCAGGGGA GAGACCTTCTCAGAGCTCTGGACCAGGCCACCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_010137 |
| Insert Size | 2625 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_010137.3, NP_034267.3 |
| RefSeq Size | 5352 bp |
| RefSeq ORF | 2625 bp |
| Locus ID | 13819 |
| UniProt ID | P97481 |
| Gene Summary | Transcription factor involved in the induction of oxygen regulated genes. Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (PubMed:26245371). Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBBP and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD (By similarity).[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR211029 | Epas1 (Myc-DDK-tagged) - Mouse endothelial PAS domain protein 1 (Epas1) |
CNY 6,400.00 |
|
| MR211029L3 | Lenti ORF clone of Epas1 (Myc-DDK-tagged) - Mouse endothelial PAS domain protein 1 (Epas1) |
CNY 8,800.00 |
|
| MR211029L4 | Lenti ORF clone of Epas1 (mGFP-tagged) - Mouse endothelial PAS domain protein 1 (Epas1) |
CNY 8,800.00 |
