Trpv4 (NM_022017) Mouse Untagged Clone

CAT#: MC222327

Trpv4 (untagged) - Mouse transient receptor potential cation channel, subfamily V, member 4 (Trpv4), (10ug)



  "NM_022017" in other vectors (6)

CNY 9,576.00


货期*
现货

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 0610033B08Rik; OTRPC4; Trp12; VR-OAC; VRL-2; VROAC
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>NCBI ORF sequence for NM_022017, the custom clone sequence may differ by one or more nucleotides


ATGGCAGATCCTGGTGATGGTCCCCGTGCAGCGCCTGGGGAGGTGGCTGAGCCCCCTGGAGATGAGAGTG
GTACCTCTGGTGGGGAGGCCTTCCCCCTCTCTTCCCTGGCCAATCTGTTTGAGGGGGAGGAAGGCTCCTC
TTCTCTTTCCCCGGTGGATGCTAGCCGCCCTGCTGGCCCTGGCGATGGACGTCCAAACCTGCGTATGAAG
TTCCAGGGCGCTTTCCGCAAGGGGGTACCCAACCCCATTGACCTGTTGGAGTCCACCCTGTACGAGTCCT
CAGTAGTGCCTGGGCCCAAGAAAGCGCCCATGGATTCCTTGTTCGACTACGGCACTTACCGTCACCACCC
CAGTGACAACAAGAGATGGAGGAGAAAGGTCGTGGAGAAGCAGCCACAGAGCCCCAAAGCTCCTGCACCC
CAGCCACCCCCCATCCTCAAAGTCTTCAATCGGCCCATCCTCTTTGACATTGTGTCCCGGGGCTCCACTG
CGGACCTAGATGGACTGCTCTCCTTCTTGTTGACCCACAAGAAGCGCCTGACTGATGAGGAGTTCCGGGA
GCCGTCCACGGGGAAGACCTGCCTGCCCAAGGCGCTGCTGAACCTAAGCAACGGGCGCAACGACACCATC
CCGGTGTTGCTGGACATTGCGGAGCGCACCGGCAACATGCGTGAATTCATCAACTCGCCCTTCAGAGACA
TCTACTACCGAGGCCAGACATCCCTGCACATTGCCATCGAACGGCGCTGCAAGCACTACGTGGAGCTGCT
GGTGGCCCAGGGAGCCGACGTGCACGCCCAGGCCCGCGGCCGCTTCTTCCAGCCCAAGGATGAGGGAGGC
TACTTCTACTTTGGGGAGCTGCCCTTGTCCCTGGCAGCCTGCACCAACCAGCCGCACATCGTCAACTACC
TGACAGAGAACCCTCACAAGAAAGCTGACATGAGGCGACAGGACTCGAGGGGGAACACGGTGCTGCACGC
GCTGGTGGCCATCGCCGACAACACCCGAGAGAACACCAAGTTTGTCACCAAGATGTACGACCTGCTGCTT
CTCAAGTGTTCACGCCTCTTCCCCGACAGCAACCTGGAGACAGTTCTCAACAATGATGGCCTTTCGCCTC
TCATGATGGCTGCCAAGACAGGCAAGATCGGGGTCTTTCAGCACATCATCCGACGTGAGGTGACAGATGA
GGACACCCGGCATCTGTCTCGCAAGTTCAAGGACTGGGCCTATGGGCCTGTGTATTCTTCTCTCTACGAC
CTCTCCTCCCTGGACACATGCGGGGAGGAGGTGTCCGTGCTGGAGATCCTGGTGTACAACAGCAAGATCG
AGAACCGCCATGAGATGCTGGCTGTAGAGCCCATTAACGAACTGTTGAGAGACAAGTGGCGTAAGTTTGG
GGCTGTGTCCTTCTACATCAACGTGGTCTCCTATCTGTGTGCCATGGTCATCTTCACCCTCACCGCCTAC
TATCAGCCACTGGAGGGCACGCCACCCTACCCTTACCGGACCACAGTGGACTACCTGAGGCTGGCTGGCG
AGGTCATCACGCTCTTCACAGGAGTCCTGTTCTTCTTTACCAGTATCAAAGACTTGTTCACGAAGAAATG
CCCTGGAGTGAATTCTCTCTTCGTCGATGGCTCCTTCCAGTTACTCTACTTCATCTACTCTGTGCTGGTG
GTTGTCTCTGCGGCGCTCTACCTGGCTGGGATCGAGGCCTACCTGGCTGTGATGGTCTTTGCCCTGGTCC
TGGGCTGGATGAATGCGCTGTACTTCACGCGCGGGTTGAAGCTGACGGGGACCTACAGCATCATGATTCA
GAAGATCCTCTTCAAAGACCTCTTCCGCTTCCTGCTTGTGTACCTGCTCTTCATGATCGGCTATGCCTCA
GCCCTGGTCACCCTCCTGAATCCGTGCACCAACATGAAGGTCTGTGACGAGGACCAGAGCAACTGCACGG
TGCCCACGTATCCTGCGTGCCGCGACAGCGAGACCTTCAGCGCCTTCCTCCTGGACCTCTTCAAGCTCAC
CATCGGCATGGGAGACCTGGAGATGCTGAGCAGCGCCAAGTACCCCGTGGTCTTCATCCTCCTGCTGGTC
ACCTACATCATCCTCACCTTCGTGCTCCTGTTGAACATGCTTATCGCCCTCATGGGTGAGACCGTGGGCC
AGGTGTCCAAGGAGAGCAAGCACATCTGGAAGTTGCAGTGGGCCACCACCATCCTGGACATCGAGCGTTC
CTTCCCTGTGTTCCTGAGGAAGGCCTTCCGCTCCGGAGAGATGGTGACTGTGGGCAAGAGCTCAGATGGC
ACTCCGGACCGCAGGTGGTGCTTCAGGGTGGACGAGGTGAACTGGTCTCACTGGAACCAGAACTTGGGCA
TCATTAACGAGGACCCTGGCAAGAGTGAAATCTACCAGTACTATGGCTTCTCCCACACCGTGGGGCGCCT
TCGTAGGGATCGTTGGTCCTCGGTGGTGCCCCGCGTAGTGGAGCTGAACAAGAACTCAAGCGCAGATGAA
GTGGTGGTACCCCTGGATAACCTAGGGAACCCCAACTGTGACGGCCACCAGCAGGGCTACGCTCCCAAGT
GGAGGACGGACGATGCCCCACTGTAG


Restriction Sites SgfI-MluI     
ACCN NM_022017
Insert Size 2616 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC127052, AAI27053
RefSeq Size 2777 bp
RefSeq ORF 2616 bp
Locus ID 63873
UniProt ID Q9EPK8
Gene Summary Non-selective calcium permeant cation channel involved in osmotic sensitivity and mechanosensitivity (PubMed:11094154). Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification (PubMed:12093812, PubMed:14691263, PubMed:16368742, PubMed:16571723). Also activated by heat, low pH, citrate and phorbol esters (PubMed:14691263). Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism (By similarity). Acts as a regulator of intracellular Ca(2+) in synoviocytes (By similarity). Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8 (By similarity). Together with PKD2, forms mechano- and thermosensitive channels in cilium (PubMed:18695040). Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers (PubMed:20413591). Negatively regulates expression of PPARGC1A, UCP1, oxidative metabolism and respiration in adipocytes (PubMed:23021218). Regulates expression of chemokines and cytokines related to proinflammatory pathway in adipocytes (PubMed:23021218). Together with AQP5, controls regulatory volume decrease in salivary epithelial cells (PubMed:16571723). Required for normal development and maintenance of bone and cartilage (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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