Piwil4 (NM_177905) Mouse Untagged Clone

CAT#: MC222360

Piwil4 (untagged) - Mouse piwi-like homolog 4 (Drosophila) (Piwil4), (10ug)



  "NM_177905" in other vectors (4)

CNY 11,210.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 9230101H05Rik; Miwi2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC222360 representing NM_177905
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGCCTGCGCATTCTGGGGGTCCACCGGGCCCTGCCCACCCACGCTCGGGTTGCTGTTTGTAGTAACT
ATCTTGGGAAGCTTGAATACTCCCAAACTCCGAGTCACAGCCACACCGTCTCTTTTGCCAAAGAGAAAAC
ACTCCTGTTGAGGCTCACCAGCCCTGGAAAGCCGCTGGCACCCAGGAACATGAGTGGACGGGCCCGTGTG
AGAGCGCGAGGCATCACCACTGGCCACAGTGCTAGGGAAGTGGGGCGGTCCTCAAGAGACCTCATGGTTA
CATCTGCCAGTCCTGGGGACAGCGAGGCCGGAGGTGGGACAAGTGTCATTTCACAGCCATATGAACTTGG
TGTCTCTTCTGGTGATGGTGGACGTACCTTCATGGAAAGAAGAGGCAAAGGCAGACAGGACTTCGAAGAG
TTGGGTGTCTGTACCAGGGAAAAGTTGACCCACGTAAAAGATTGTAAAACAGGTTCTAGCGGAATACCTG
TGCGGCTGGTTACAAATCTCTTTAATTTAGATTTGCCCCAGGATTGGCAGCTGTACCAGTACCATGTCAC
GTACAGCCCAGATTTAGCCTCTAGGAGGCTGAGGATTGCTTTGCTTTATAATCACAGCATACTCTCAGAC
AAGGCAAAAGCATTTGACGGTGCCAGCCTTTTCCTCTCAGAAAAGCTGGACCAGAAGGTCACAGAACTGA
CAAGTGAAACACAAAGAGGTGAGACTATAAAGATAACGCTCACCCTAACGAGCAAGCTCTTCCCAAACTC
CCCTGTGTGCATTCAGTTCTTCAATGTCATCTTCAGAAAGATCTTGAAAAACTTGAGTATGTACCAAATT
GGACGGAACTTCTATAAGCCTTCAGAACCAGTGGAGATCCCTCAGTACAACAAGCTCCTATTCAATGCTG
ACGTGAACTACAAAGTCCTCCGCAATGAGACCGTTCTGGACTTCATGACTGATCTCTGCCTCAGAACTGG
CATGTCTTGCTTCACCGAGATGTGCCACAAACAACTAGTGGGGCTCGTTGTCCTTACCAGATACAACAAC
AAAACATATCGTATCGATGACATTGACTGGTCTGTGAAGCCCACACAGGCCTTTCAGAAGCGGGACGGCT
CAGAGGTCACATATGTGGATTACTACAAGCAGCAATATGATATCACCTTATCTGACCTAAATCAGCCAGT
GCTTGTTAGTCTGTTGAAAAGAAAGAGAAATGATAACTCTGAGCCTCAGATGGTCCACCTGATGCCAGAG
CTCTGCTTTCTCACAGGCCTGAGCAGCCAAGCAACCTCAGATTTCCGCCTGATGAAGGCAGTAGCTGAAG
AGACTCGGCTCAGTCCTGTGGGAAGGCAGCAGCAGCTGGCCCGACTCGTGGATGACATCCAGAGAACCTT
ACCTTCTTCTCAAGAAGTTCTCTCTCATACTTCCTTGCCTTTGTGGGCCCCAGAGCCAGGAGGACTTAGC
AGTGCTATACCACTGAGCACTGTGCTTCCCTTTGCCCAGCAGCTGTTAACTGCCCTGAGCCTCTCCCCGG
GAATCCCTCTGCCCCACCTCAAGCCTCCCTCCTTCCTGTTCTTGTGTCAACCTGCATTTGCTGCTGACTG
GTCCAAAGATATGCGATCTTGCAAAGTTTTGAGTTCTCAGCCTTTGAATAGATGGTTGATCGTGTGCTGT
AACAGGGCTGAGCACTTGATTGAAGCCTTTCTGAGCTGTCTGAGGAGAGTTGGAGGTTCCATGGGATTTA
ACGTGGGCTACCCCAAAATCATAAAAGTGGACGAGACCCCAGCAGCGTTCCTTCGAGCCATCCAGGTGCA
CGGCGACCCCGATGTTCAGTTGGTGATGTGCATTCTGCCTTCTAATCAGAAGAACTATTACGACTCCATT
AAAAAGTATTTGAGCTCTGACTGCCCAGTGCCAAGCCAGTGTGTGCTGACCCGGACCTTGAATAAGCAGG
GAACGATGCTGAGTGTGGCCACCAAGATCGCCATGCAGATGACCTGCAAACTTGGCGGAGAGCTGTGGTC
TGTGGAGATCCCATTGAAGTCCCTGATGGTCGTGGGTATTGATATCTGCAGAGATGCCCTCAACAAGAAT
GTGGTGGTCGTCGGGTTTGTAGCCAGCATTAATTCCAGGATCACCAGGTGGTTTTCCCGCTGTGTCCTTC
AGAGAACAGCGGCTGATATTGCAGATTGCCTCAAAGTCTGCATGACTGGTGCTCTCAACCGGTGGTACAG
ACACAACCATGACTTGCCGGCACGGATAGTCGTGTACCGGGACGGTGTAGGCAATGGCCAGCTAAAGGCA
GTTTTGGAATATGAAGTCCCACAGCTACTGAAAAGTGTAACAGAGTGCGGCTCGGATGCCAGGTATGACT
TCTACCTGATCAGCCAGACTGCTAACCGGGGGACTGTTAGTCCCACCCACTACAACGTTATCTATGATGA
CAATGCCTTGAAGCCTGACCACATGCAGCGACTGACCTTCAAACTGTGCCATCTCTACTACAACTGGCAG
GGCTTAATCAGTGTCCCCGCACCATGCCAATATGCACACAAGCTGACCTTCCTGGTGGCACAAAGTGTCC
ACAAGGAACCAAGTCTGGAATTAGCCAATAATCTTTTCTACCTCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_177905
Insert Size 2637 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_177905.3, NP_808573.2
RefSeq Size 2637 bp
RefSeq ORF 2637 bp
Locus ID 330890
UniProt ID Q8CGT6
Gene Summary Plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity (PubMed:17395546, PubMed:18381894, PubMed:18922463, PubMed:26669262, PubMed:22020280). Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons (PubMed:17395546, PubMed:18381894, PubMed:18922463, PubMed:26669262, PubMed:22020280). Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with secondary piRNAs antisense and PIWIL2/MILI is required for such association (PubMed:17395546, PubMed:18381894, PubMed:18922463, PubMed:26669262, PubMed:22020280). The piRNA process acts upstream of known mediators of DNA methylation (PubMed:17395546, PubMed:18381894, PubMed:18922463, PubMed:26669262, PubMed:22020280). Does not show endonuclease activity (PubMed:22020280). Plays a key role in the piRNA amplification loop, also named ping-pong amplification cycle, by acting as a 'slicer-incompetent' component that loads cleaved piRNAs from the 'slicer-competent' component PIWIL2 and target them on genomic transposon loci in the nucleus (PubMed:22020280). In addition to its role in germline, PIWIL4 also plays a role in the regulation of somatic cells activities. Plays a role in pancreatic beta cell function and insulin secretion (By similarity). Involved in maintaining cell morphology and functional integrity of retinal epithelial through Akt/GSK3alpha/beta signaling pathway (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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