Cdh1 (NM_009864) Mouse Untagged Clone

CAT#: MC222365

Cdh1 (untagged) - Mouse cadherin 1 (Cdh1), (10ug)



  "NM_009864" in other vectors (4)

CNY 11,310.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
Cdh1 Antibody - C-terminal region
    • 50 ug

CNY 4,628.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AA960649; ARC-1; E-ca; E-cad; E-cadh; Eca; Ecad; L-C; L-CAM; Um; UVO
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC222365 representing NM_009864
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGAGCCCGGTGCCGCAGCTTTTCCGCGCTCCTGCTCCTGCTGCAGGTCTCCTCATGGCTTTGCCAGG
AGCTGGAGCCTGAGTCCTGCAGTCCCGGCTTCAGTTCCGAGGTCTACACCTTCCCGGTGCCGGAGAGGCA
CCTGGAGAGAGGCCATGTCCTGGGCAGAGTGAGATTTGAAGGATGCACTGGCCGGCCAAGGACAGCCTTC
TTTTCGGAAGACTCCCGATTCAAAGTGGCGACAGACGGCACCATCACAGTGAAGCGGCATCTAAAGCTCC
ACAAGCTGGAGACCAGTTTCCTCGTCCGCGCCCGGGACTCCAGTCATAGGGAGCTGTCTACCAAAGTGAC
GCTGAAGTCCATGGGGCACCACCATCACCGGCACCACCACCGCGACCCTGCCTCTGAATCCAACCCAGAG
CTGCTCATGTTTCCCAGCGTGTACCCAGGTCTCAGAAGACAGAAACGAGACTGGGTCATCCCTCCCATCA
GCTGCCCCGAAAATGAAAAGGGCGAATTTCCAAAGAACCTGGTTCAGATCAAATCCAACAGGGACAAAGA
AACAAAGGTTTTCTACAGCATCACCGGCCAAGGAGCTGACAAACCCCCCGTTGGCGTTTTCATCATTGAG
AGGGAGACAGGCTGGCTGAAAGTGACACAGCCTCTGGATAGAGAAGCCATTGCCAAGTACATCCTCTATT
CTCATGCCGTGTCATCAAATGGGGAAGCGGTGGAGGATCCCATGGAGATAGTGATCACAGTGACAGATCA
GAATGACAACAGGCCAGAGTTTACCCAGCCGGTCTTTGAGGGATTCGTTGCAGAAGGCGCTGTTCCAGGA
ACCTCCGTGATGAAGGTCTCAGCCACCGATGCAGACGATGACGTCAACACCTACAACGCTGCCATCGCCT
ACACCATCGTCAGCCAGGATCCTGAGCTGCCTCACAAAAACATGTTCACTGTCAATAGGGACACCGGGGT
CATCAGTGTGCTCACCTCTGGGCTGGACCGAGAGAGTTACCCTACATACACTCTGGTGGTTCAGGCTGCT
GACCTTCAAGGTGAAGGCTTGAGCACAACAGCCAAGGCTGTGATCACTGTCAAGGATATTAATGACAACG
CTCCTGTCTTCAACCCAAGCACGTATCAGGGTCAAGTGCCTGAGAATGAGGTCAATGCCCGGATCGCCAC
ACTCAAAGTGACCGATGATGATGCCCCCAACACTCCGGCGTGGAAAGCTGTGTACACCGTAGTCAACGAT
CCTGACCAGCAGTTCGTTGTTGTCACAGACCCCACGACCAATGATGGCATTTTGAAAACAGCCAAGGGCT
TGGATTTTGAGGCCAAGCAGCAATACATCCTTCATGTGAGAGTGGAGAACGAGGAACCCTTTGAGGGGTC
TCTTGTCCCTTCCACAGCCACTGTCACTGTGGACGTGGTAGACGTGAATGAAGCCCCCATCTTTATGCCT
GCGGAGAGGAGAGTCGAAGTGCCCGAAGACTTTGGTGTGGGTCAGGAAATCACATCTTATACCGCTCGAG
AGCCGGACACGTTCATGGATCAGAAGATCACGTATCGGATTTGGAGGGACACTGCCAACTGGCTGGAGAT
TAACCCAGAGACTGGTGCCATTTTCACGCGCGCTGAGATGGACAGAGAAGACGCTGAGCATGTGAAGAAC
AGCACATATGTAGCTCTCATCATCGCCACAGATGATGGTTCACCCATTGCCACTGGCACGGGCACTCTTC
TCCTGGTCCTGTTAGACGTCAATGACAACGCTCCCATCCCAGAACCTCGAAACATGCAGTTCTGCCAGAG
GAACCCACAGCCTCATATCATCACCATCTTGGATCCAGACCTTCCCCCCAACACGTCCCCCTTTACTGCT
GAGCTAACCCATGGGGCCAGCGTCAACTGGACCATTGAGTATAATGACGCAGCTCAAGAATCTCTCATTT
TGCAACCAAGAAAGGACTTAGAGATTGGCGAATACAAAATCCATCTCAAGCTCGCGGATAACCAGAACAA
AGACCAGGTGACCACGTTGGACGTCCATGTGTGTGACTGTGAAGGGACGGTCAACAACTGCATGAAGGCG
GGAATCGTGGCAGCAGGATTGCAAGTTCCTGCCATCCTCGGAATCCTTGGAGGGATCCTCGCCCTGCTGA
TTCTGATCCTGCTGCTCCTACTGTTTCTACGGAGGAGAACGGTGGTCAAAGAGCCCCTGCTGCCACCAGA
TGATGATACCCGGGACAATGTGTATTACTATGATGAAGAAGGAGGTGGAGAAGAAGACCAGGACTTTGAT
TTGAGCCAGCTGCACAGGGGCCTGGATGCCCGACCGGAAGTGACTCGAAATGATGTGGCTCCCACCCTCA
TGAGCGTGCCCCAGTATCGTCCCCGTCCTGCCAATCCTGATGAAATTGGAAACTTCATCGATGAAAACCT
GAAGGCAGCCGACAGCGACCCCACGGCACCCCCTTACGACTCTCTGTTGGTGTTCGATTACGAGGGCAGT
GGTTCTGAAGCCGCTAGCCTGAGCTCACTGAACTCCTCTGAGTCGGATCAGGACCAGGACTACGATTATC
TGAACGAGTGGGGCAACCGATTCAAGAAGCTGGCGGACATGTACGGCGGTGGTGAGGACGACTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_009864
Insert Size 2655 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_009864.3, NP_033994.1
RefSeq Size 4447 bp
RefSeq ORF 2655 bp
Locus ID 12550
UniProt ID P09803
Gene Summary This gene encodes E-cadherin, a calcium-dependent cell adhesion molecule that functions in the establishment and maintenance of epithelial cell morphology during embryongenesis and adulthood. The encoded preproprotein undergoes proteolytic processing to generate a mature protein. Targeted mutations disrupting binding of calcium to the encoded protein in mice cause death in utero due to failed blastocyst and trophectoderm formation. This gene is located adjacent to a related cadherin gene on chromosome 8. [provided by RefSeq, Oct 2015]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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